排序方式: 共有189条查询结果,搜索用时 265 毫秒
71.
Burhanettin İmrak Ali Küden Veyis Yurtkulu Ebru Kafkas Sezai Ercişli Salih Kafkas 《Biochemical genetics》2017,55(3):234-243
In this study, promising candidates of six apricot genotypes were compared with our local and major cultivar (Hac?halilo?lu) based on their phenological, pomological, and biochemical characteristics. Fruit weight, stone weight, flesh firmness, flesh fruit/stone ratio, TSS (Total soluble solids), glucose, fructose, sucrose, total phenols, total antioxidant activity (FRAP and ABTS assays) and bud break, first bloom, full bloom, and harvesting date of apricot cultivar candidates were compared with cultivar Hac?halilo?lu ‘HH.’ According to the obtained results, the higher content of total phenolic compounds and total antioxidant activity was determined in the N95 genotype (96.87 µmol TE/g) fruit, whereas the ‘HH’ cultivar was characterized by the lower value (94.6 µmol TE/g) especially determined by FRAP method. Individual sugars and the soluble solids content of fruit differed between selected genotypes and ‘HH’ cultivar. Glucose, fructose, and sucrose contents were higher in all selected genotypes than in Hac?halilo?lu cultivar. 相似文献
72.
Mortaza Khodaeiaminjan Ebru Kafkas Murat Güney Salih Kafkas 《Molecular breeding : new strategies in plant improvement》2017,37(8):98
The dioecious character of Pistacia vera L (the pistachio tree) limits its breeding capacity. Thus, early stage selection of males can save time, labor, and land. This study aimed to develop sex-linked single nucleotide polymorphism (SNP) markers, together with expressed sequence tag-derived simple sequence repeats (EST-SSRs), to determine position of the sex locus in pistachio by constructing a linkage map of its sex chromosome for the first time. Nine novel sex-linked SNP markers were successfully identified by SNaPshot minisequencing analysis of 25 SNP loci from 17 restriction site-associated DNA (RAD) reads in 309 individuals. All nine markers were heterozygous in females and homozygous in males supporting a ZW/ZZ sex determination system in pistachio. A total of 105 segregating SSRs and sex-linked markers were used to identify the sex chromosome and the position of the sex locus through analysis of a Siirt × Ba?yolu F1 population with 122 progenies. Of these 105 markers, four common and four paternal SSRs were mapped onto the sex chromosome, along with the phenotypic sex locus and sex-linked markers. The resulting consensus map had a total length of 65.19 cM. The sex locus and sex-linked SNP markers were located in the center of the chromosome at a distance of 31.86 and 31.92 cM, respectively. This study presents valuable information about the sex chromosome and sex locus position as well as novel polymorphic EST-SSRs and nine sex-linked SNP markers in pistachio. 相似文献
73.
Nitric oxide production in newborns under phototherapy. 总被引:2,自引:0,他引:2
Ebru Ergenekon Kivilcim Gücüyener Hasan Dursun Deniz Erba? Güler Oztürk Esin Ko? Yildiz Atalay 《Nitric oxide》2002,6(1):69-72
Nitrogen monoxide (NO) is a potent endogenous vasodilator and is involved in cytotoxicity, neurotransmission, and immunological defense mechanisms. Phototherapy has long been known to change the distribution of blood flow throughout the body in newborn infants. The objective of this study was to investigate the effect of phototherapy on NO production in otherwise healthy newborns. Urinary NO levels were measured before and 6 h after phototherapy by a chemiluminescence method using Sievers NOA. Ten newborns (gestational age, 36.4 +/- 3.9 weeks; birth weight, 2863 +/- 677.44 g; postnatal age, 5.1 +/- 2.72 days) were started on phototherapy according to AAP guidelines and urine for NO measurement was collected prior to therapy and 6 h after the commencement of treatment. Urinary NO levels measured during phototherapy were significantly higher (108.8+/-50.69 micromol/mmol creatinine) than the levels measured before phototherapy (73.13+/-34.15 micromol/mmol creatinine; P < 0.05). These results suggest that newborns receiving phototherapy might have increased NO production, which might result in hemodynamic changes. However, further studies on the effects of phototherapy on NO and photorelaxation are needed before reaching firm conclusions. 相似文献
74.
75.
76.
Annarita Poli Hande Kazak Bahar Gürleyendağ Giuseppina Tommonaro Giuseppina Pieretti Ebru Toksoy Öner Barbara Nicolaus 《Carbohydrate polymers》2009,78(4):651-657
A novel halophilic isolate from soil samples taken from Çamalt? Saltern area in Turkey, Halomonas sp. AAD6 (JCM 15723) strain, was found to produce high levels of exopolysaccharides (EPS), in the presence of sucrose in defined media, by flasks and bioreactor condition yielded 1.073 g L?1 and 1.844 g L?1, respectively. Sugar analysis, methylation studies and NMR analysis of EPS indicated the repeating unit of this polysaccharide was composed of β-(2,6)-d-fructofuranosyl residues. Hence with this work, Halomonas sp. has been described as a levan producer microorganism for the first time. Biocompatibility studies showed this EPS did not affect cellular viability and proliferation of osteoblasts and murine macrophages. The protective effect of the polymer against the toxic activity of avarol implied its additional use as an anti-cytotoxic agent. Halomonas sp. AAD6 could represent an alternative cheap source of levan polymer when grown on defined media hypothesizing its larger employment in industrial application being non pathogenic microorganism. 相似文献
77.
Fikret Karatas Ebru Coteli Suleyman Aydin Suleyman Servi Haki Kara 《Biological trace element research》2010,136(1):79-86
The influence of injection periods of 3-(1H-pyrrol-2-yl)-1H-indazole regarding vitamins A, E, C, selenium (Se), malondialdehyde (MDA) levels, and glutathione peroxidase (GSH-Px) activity
in rats has been investigated. The substance was given by subcutaneous injection at 20 mg/kg every other day for a total of
15 injections. At the end of the treatment, Se levels in serum were determined by fluorimetry, and those of vitamins A, E,
C, and malondialdehyde in serum, liver, and kidney were determined by high-performance liquid chromatography. GSH-Px activities
in erythrocytes were determined spectrophotometrically. Vitamins A, E, C, and Se levels were generally lower than in the controls,
while GSH-Px activity at the third injection period was maximally increased, with the activities after the other injection
periods being higher than in the control group. In addition, vitamins A, E, and C levels were generally lower than the control
groups, while serum, liver, and kidney MDA levels gradually increased depending on injection periods. On the other hand, GSH-Px
activity was higher than in the control group. Thus, the results show that while vitamins A, E, C, and Se levels decreased,
MDA levels and GSH-Px activities increased after administration of 3-(1H-pyrrol-2-yl)-1H-indazole to the rats. These findings might be related to the increased amount of free radicals caused by 3-(1H-pyrrol-2-yl)-1H-indazole injection. 相似文献
78.
Eukaryotic cells can sense a wide variety of environmental stresses, including changes in temperature, pH, osmolarity and
nutrient availability. They respond to these changes through a variety of signal-transduction mechanisms, including activation
of Ca2+-dependent signaling pathways. This research has discovered important implications in the function(s) of polycystic kidney
disease (PKD) channels and the mechanisms through which they act in the control of cell growth and cell polarity in Schizosaccharomyces pombe by ion channel-mediated Ca2+ signaling. Pkd2 was expressed maximally during the exponential growth phase. At the cell surface pkd2 was localized at the cell tip
during the G2 phase of the cell cycle, although following cell wall damage, the cell surface-expressed protein relocalized to the whole
plasma membrane. Pkd2 depletion affected Golgi trafficking, resulting in a buildup of vesicles at the cell poles, and strongly
affected plasma membrane protein delivery. Surface-localized pkd2 was present in the plasma membrane for a very short time
and was rapidly internalized. Internalization was dependent on Ca2+, enhanced by amphipaths and inhibited by gadolinium. The pkd2 protein was in a complex with a yeast synaptotagmin homologue
and myosin V. Depletion of pkd2 severely affected the localization of glucan synthase. A role for pkd2 in a cell polarity
and cell wall synthesis signaling complex with a synaptotagmin homologue, myosin V and glucan synthase is proposed. 相似文献
79.
Mete E Gul HI Canturk P Topcu Z Pandit B Gul M Li PK 《Zeitschrift für Naturforschung. C, Journal of biosciences》2010,65(11-12):647-652
A number of studies reported Mannich bases to manifest antimicrobial, cytotoxic, anticancer, anti-inflammatory, and anticonvulsant activities. A considerable number of therapeutically important cytotoxic compounds are active on DNA topoisomerases that regulate the DNA topology. In the present study we evaluated the biological activity of mono-Mannich bases, 1-aryl-3-phenethylamino-1-propanone hydrochlorides (1a-10a), and semicyclic mono-Mannich bases, 3-aroyl-4-aryl-1-phenethyl-4-piperidinols (1b-9b), synthesized in our laboratory. We employed androgen-independent human prostate cancer cells (PC-3) to assess the cytotoxicity of the compounds and extended the biological activity evaluation to cover supercoil relaxation assays of mammalian type I topoisomerases. Our results showed that the compounds had cytotoxicity within the 8.2-32.1 microM range, while two compounds gave rise to a comparable average value in topo I interference of 42% and 40% for 10a (with a hydroxy substituent on the phenyl ring from mono-Mannich bases) and 5b (with a fluoro substituent on the phenyl ring from the semicyclic mono-Mannich base series, piperidinols), respectively. 相似文献
80.