Distinction of strains of bean common mosaic virus and blackeye cowpea mosaic virus using antibodies to N- and C- or N-terminal peptide domains of coat proteins

Earlier attempts to discriminate serologically strains NL1, NL3 and NY15 of bean common mosaic virus (BCMV) and strain W of blackeye cowpea mosaic virus (B1CMV) had been unsuccessful. Antibodies directed towards N- and C-, or N-terminal peptide regions of the coat proteins of the above strains enabled the distinction between B1CMV-W, BCMV-NY15 and BCMV-NL3 in electroblot immunoassay and in ELISA. The distinction was better with antibodies directed towards N-termini than with those to N- and C-termini. Strain NL1 of BCMV cross-reacted with both B1CMV-W and BCMV-NY15, but not with BCMV-NL3. Taxonomic implications of these findings are discussed.     

A SALT-TOLERANT DENITRIFYING BACILLUS STRAIN WHICH 'BLOWS' CANNED BACON

SUMMARY: A Bacillus strain, isolated from a 'blown' can of bacon, is described: it tolerates 15% NaCl and reduces nitrate and nitrite to N₂O and N₂. The pH-salt relationship, the tolerance to nitrite under aerobic and anaerobic conditions, the heat resistance of the spores, and the composition of the gas produced, have been investigated. The characters of the organism are such that, should its spores survive the processing of a cured meat, their subsequent development and the spoilage of the product seem almost certain.