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31.
Richard S. Dodd Franois Fromard Zara A. Rafii Franois Blasco 《Biochemical Systematics and Ecology》1995,23(7-8):859-868
Foliage from 21 red mangroves (Rhizophora mangle, R. racemosa and R. x harrisonii) from different ecogeographic conditions in Gabon, West Africa, was analyzed for epicuticular wax composition using CC, GC and GC-MS. Aliphatic hydrocarbons ranging from C23 to C34 and some triterpenoids were identified. Alkanes were the major constituents (46.7–99.9%), with triterpenoids also accounting for up to 53.3% of the wax extract. High contents of octacosane (27.2%) and lower amounts of nonacosane (14.9%) and hentriacontane (9.8%) distinguished R. mangle from the other two species. R. hizophora x harrisonii was exceptionally rich in nonacosane (45.3%) with moderately high concentrations of hentriacontane (25.4%), whereas R. racemosa was intermediate between the two former species in its content of these three alkanes. Inclusion of the less abundant constituents in principal components analysis provided a good separation of R. x harrisonii, whereas the two other species showed some degree of overlap. Although major alkane patterns can be used to discriminate among the Rhizophora species examined, we do find substantial intra-specific variation that may be attributable to population genetic variation; this was least for R. x harrisonii. The hybrid status of this latter species could not be confirmed from the biochemical analyses carried out. 相似文献
32.
Studies on the subcellular distribution of immunoreactive cholecystokinin (CCK) in homogenates of rat cerebral cortex showed that approximately 95% was associated with particulate fractions, including presynaptic terminals (synaptosomes). Chromatography of extracts of tissue and medium from incubated synaptosomes revealed that this material was almost exclusively in the form of COOH-terminal octapeptide (CCK-8), very little CCK-33 being present. There was a wide range of CCK-8 concentrations in synaptosomes from different brain regions (cortex > striatum ? hypothalamus > brain stem). Cerebral cortex synaptosomes were incubated in vitro and showed a complex pattern of CCK-8 release with varying concentrations of tissue: amounts in the medium rose rapidly with increasing synaptosome concentrations, then fell to a plateau at higher tissue values. A mechanism for the rapid disposal of extracellular CCK-8 was associated with synaptosomal fractions. Depolarization-induced (high K+) release of CCK-8 was observed with cortex and corpus striatum synaptosomes. A rapid and reversible enhancement of CCK-8 release from cortex slices was observed in response to elevated K+. Veratrine also released CCK-8 from cortex slices, although this was not reversible. Stimulus-induced release of CCK-8 from synaptosomes and slices required extracellular Ca2+. The storage, release and degradation of CCK-8 by nerve-endings suggest a synaptic function for this peptide. 相似文献
33.
Nitrogen budget of a shortgrass prairie ecosystem 总被引:3,自引:0,他引:3
Summary A N budget is presented for a shortgrass prairie ecosystem. The grassland was ungrazed by domestic herbivores. The quantities of N in various plant, animal, microorganism, and soil components of the ecosystem are estimated for the date when aboveground living biomass was at its maximum for the growing season of 1973. Annual transfers of N between the various compartments were also estimated.Of the total N, 99.5% was in organic forms. The relatively inert heteropolycondensate fraction of the organic matter in the soils contained 88.8% of the N. Living organisms contained 4.2% and dead but recognizable organisms or part thereof contained 6.5% of the total N. Belowground animals contained more than 10 times as much N as abovegroud animals, but combined, animals contained less than 0.1% of the total in the system. Living plant material contained 2.5% of the total N. Seventy-two percent of the living plant N was below ground. Microorganisms contained 1.4% of the total N.Total N uptake by plants from soil solution was 2.9 g·m-2·yr-1. Aerial portions of plants were allocated 1.9 N·m-2·yr-1 although apparently 26% of this amount came from internally recycled sources. The heteropolycondensate fraction of the soil contributed 0.7 g N·m-2·yr-1 to mineral forms, but these components of the system were assumed to be in steady state; thus an equal amount of mineral N was allocated back to the source. Mineralization of N from plant residues was sufficient to account for all of the N taken up by plants from soil solution. Soil animals immobilized about 0.4 g N·m-2·yr-1 while the amount shunted to aboveground animals was trivial. 相似文献
34.
Increased recovery of salmonellae from environmental samples enriched with buffered peptone water. 总被引:14,自引:10,他引:4 下载免费PDF全文
The incidence and persistence of salmonellae in weather pools on the top of Stone Mountain were investigated with lactose and buffered peptone water used as pre-enrichment broths. A total of 162 samples were collected from 16 weather pools over a 3-month period. The use of buffered peptone water increased the recovery of salmonellae by approximately 25%. The combined use of direct enrichment in tetrathionate broth containing brilliant green dye and pre-enrichment in buffered peptone water followed by enrichment in tetrathionate broth made it possible to detect all 37 of the contaminated samples. All of the isolates were Salmonella bareilly, the only serotype recovered in a previous study. All but one of the isolations were made from moist or wet samples. S. bareilly was isolated from rabbit dung and litter collected near the weather pools, but attempts to trap rabbits for study were unsuccessful. Random samples taken along a side of the mountain yielded S. bareilly in weather pools within the upper third of the mountain; below this level, S. weslaco and S. memphis were recovered, but not S. bareilly. 相似文献
35.
Jill E. Maddison Wendy E. J. Watson Peter R. Dodd Graham A. R. Johnston 《Journal of neurochemistry》1991,56(6):1881-1888
Excitatory amino acid receptor binding parameters were investigated in a spontaneous dog model of chronic hepatic encephalopathy. L-[3H]Glutamate, (+)-[3H]-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-im ine maleate ([3H]MK-801), [3H]kainate, and alpha-[3H]-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) binding experiments were performed using crude cerebrocortical synaptosomal membrane preparations from dogs with congenital portosystemic encephalopathy (PSE) and control dogs. There was no change in the affinity or density of L-[3H]-glutamate or [3H]MK-801 binding sites in dogs with congenital PSE compared with control dogs. However, in the PSE dogs there was a significant reduction in the density of [3H]kainate binding sites compared with control dogs and abolition of the low-affinity [3H]AMPA binding site. The relative binding capacity of PSE synaptosomal membranes for [3H]kainate and [3H]AMPA was expressed as the ratio Bmax/KD. There was a significant inverse correlation between the Bmax/KD ratio for [3H]AMPA binding and the worst grade of encephalopathy experienced by each dog. These results suggest that there is a significant perturbation of cerebrocortical non-N-methyl-D-aspartate receptor binding in dogs with congenital PSE which may have relevance to the pathogenesis of hepatic encephalopathy. 相似文献
36.
Peter R. Dodd Gregory J. Thomas Clive G. Harper Jillian J. Kril 《Journal of neurochemistry》1992,59(4):1506-1515
Gamma-aminobutyric acidA/benzodiazepine receptor binding sites and the N-methyl-D-aspartate subclass of glutamate receptor sites were assessed in synaptic plasma membrane homogenates of cerebral cortex tissue obtained at autopsy from cirrhotic and noncirrhotic alcoholic patients and matched control subjects. The alcoholic patients consumed an average of greater than 80 g of ethanol/day, the control subjects less than 20 g/day. Postmortem delays up to approximately 100 h caused no significant loss of any of the binding sites; the patient and subject groups were closely matched for age. The affinities (KD) of the receptor sites did not differ between the patient and subject groups, nor between cortical regions. Using three different radioligands ([3H]muscimol, [3H]flunitrazepam, and [3H]diazepam), the gamma-aminobutyric acidA/benzodiazepine receptor complex was found to have greater density (Bmax) in superior frontal gyrus in alcoholic patients (which selectively shows morphological change in alcoholic patients), but was unchanged in motor cortex. Alcoholic patients with cirrhosis had much less pronounced changes. The density of the N-methyl-D-aspartate subclass of glutamate receptors, assessed with [3H]MK-801, did not vary across patient and subject groups. 相似文献
37.
Hensen's node induces neural tissue in Xenopus ectoderm. Implications for the action of the organizer in neural induction. 总被引:3,自引:0,他引:3
The development of the vertebrate nervous system is initiated in amphibia by inductive interactions between ectoderm and a region of the embryo called the organizer. The organizer tissue in the dorsal lip of the blastopore of Xenopus and Hensen's node in chick embryos have similar neural inducing properties when transplanted into ectopic sites in their respective embryos. To begin to determine the nature of the inducing signals of the organizer and whether they are conserved across species we have examined the ability of Hensen's node to induce neural tissue in Xenopus ectoderm. We show that Hensen's node induces large amounts of neural tissue in Xenopus ectoderm. Neural induction proceeds in the absence of mesodermal differentiation and is accompanied by tissue movements which may reflect notoplate induction. The competence of the ectoderm to respond to Hensen's node extends much later in development than that to activin-A or to induction by vegetal cells, and parallels the extended competence to neural induction by axial mesoderm. The actions of activin-A and Hensen's node are further distinguished by their effects on lithium-treated ectoderm. These results suggest that neural induction can occur efficiently in response to inducing signals from organizer tissue arrested at a stage prior to gastrulation, and that such early interactions in the blastula may be an important component of neural induction in vertebrate embryos. 相似文献
38.
E M Orozco K E Rushlow J R Dodd R B Hallick 《The Journal of biological chemistry》1980,255(22):10997-11003
39.
E J Fogt L M Dodd A R Eddy E Jenning A H Clemens 《Hormone and metabolic research. Supplement series》1979,(8):18-24
The BIOSTATOR Systems have an on-line glucose analyzer for use with whole blood. This analyzer utilizes a novel enzyme (glucose oxidase) membrane configuration and an electrochemical cell to measure the H2O2 generated. The analyzer response is fast, accurate, precise, stable, and linearly related to the blood glucose concentration over the full range of clinical interest. Extensive correlation studies have been completed to show the agreement between this analyzer and the U.S. Food and Drug Administration's recommended hexokinase-glucose-6-phosphate dehydrogenase procedure. In addition, studies on potentially interfering substance and the differences in whole blood and plasma glucose levels have been completed. 相似文献
40.
J Z Heckmatt A B Houston D J Clow J B Strephenson K L Dodd G T Lealman R W Logan 《BMJ (Clinical research ed.)》1976,1(6009):559-561
One-hundred-sixty-five children without known neurological disorder who presented with their first febrile convulsion between the ages of six months and three years were assigned to daily phenobarbitone treatment or to a control group and followed up at a special clinic for six months. One-hundred-and-sixty-one-one children completed the trial, and of the 88 children assigned to phenobarbitone treatment 10 had further convulsions during this period compared with 14 of the 73 control children. Only 49 of those assigned to phenobarbitone took the drug regularly throughout the trial, and four of these had further febrile convulsions, a proportion not significantly different from that in the controls. All four had mean plasma phenobarbitone concentrations over 69 mumol/l (16 mug/ml) during the trial and in three the plasma concentration was at or over this figure within eight hours over 69 mumol/l (16 mug/ml) during the trial and in three the plasma concentration was at or over this figure within eight hours of the repeat convulsion. Regular phenobarbitone does not seem to prevent febrile convulsions. Attention should instead be directed to organising emergency services to allow early termination of fevrile convulsions, whether first or subsequent, to prevent irreversible brain damage. 相似文献