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31.
Over 450 transfer RNA (tRNA) genes have been annotated in the human genome. Reliable quantitation of tRNA levels in human samples using microarray methods presents a technical challenge. We have developed a microarray method to quantify tRNAs based on a fluorescent dye-labeling technique. The first-generation tRNA microarray consists of 42 probes for nuclear encoded tRNAs and 21 probes for mitochondrial encoded tRNAs. These probes cover tRNAs for all 20 amino acids and 11 isoacceptor families. Using this array, we report that the amounts of tRNA within the total cellular RNA vary widely among eight different human tissues. The brain expresses higher overall levels of nuclear encoded tRNAs than every tissue examined but one and higher levels of mitochondrial encoded tRNAs than every tissue examined. We found tissue-specific differences in the expression of individual tRNA species, and tRNAs decoding amino acids with similar chemical properties exhibited coordinated expression in distinct tissue types. Relative tRNA abundance exhibits a statistically significant correlation to the codon usage of a collection of highly expressed, tissue-specific genes in a subset of tissues or tRNA isoacceptors. Our findings demonstrate the existence of tissue-specific expression of tRNA species that strongly implicates a role for tRNA heterogeneity in regulating translation and possibly additional processes in vertebrate organisms. 相似文献
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Microbial and chemical characterization of underwater fresh water springs in the Dead Sea 总被引:1,自引:0,他引:1
Ionescu D Siebert C Polerecky L Munwes YY Lott C Häusler S Bižić-Ionescu M Quast C Peplies J Glöckner FO Ramette A Rödiger T Dittmar T Oren A Geyer S Stärk HJ Sauter M Licha T Laronne JB de Beer D 《PloS one》2012,7(6):e38319
Due to its extreme salinity and high Mg concentration the Dead Sea is characterized by a very low density of cells most of which are Archaea. We discovered several underwater fresh to brackish water springs in the Dead Sea harboring dense microbial communities. We provide the first characterization of these communities, discuss their possible origin, hydrochemical environment, energetic resources and the putative biogeochemical pathways they are mediating. Pyrosequencing of the 16S rRNA gene and community fingerprinting methods showed that the spring community originates from the Dead Sea sediments and not from the aquifer. Furthermore, it suggested that there is a dense Archaeal community in the shoreline pore water of the lake. Sequences of bacterial sulfate reducers, nitrifiers iron oxidizers and iron reducers were identified as well. Analysis of white and green biofilms suggested that sulfide oxidation through chemolitotrophy and phototrophy is highly significant. Hyperspectral analysis showed a tight association between abundant green sulfur bacteria and cyanobacteria in the green biofilms. Together, our findings show that the Dead Sea floor harbors diverse microbial communities, part of which is not known from other hypersaline environments. Analysis of the water's chemistry shows evidence of microbial activity along the path and suggests that the springs supply nitrogen, phosphorus and organic matter to the microbial communities in the Dead Sea. The underwater springs are a newly recognized water source for the Dead Sea. Their input of microorganisms and nutrients needs to be considered in the assessment of possible impact of dilution events of the lake surface waters, such as those that will occur in the future due to the intended establishment of the Red Sea-Dead Sea water conduit. 相似文献
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Alaoui-Ismaili O.; Vernet-Maury E.; Dittmar A.; Delhomme G.; Chanel J. 《Chemical senses》1997,22(3):237-248
The aim of this paper is to analyse the relationship betweenself-report hedonic evaluations and the physiological expressionof emotion in response to odorants. We try to solve the followingquestions: (1) Is it possible to find any experimental evidencethat the sense of smell is linked with emotion? (2) What kindof odorants can be distinguished by autonomic analysis? (3)Is there a link between hedonics and autonomic information?The effects of odorants on the emotional process were estimated,in terms of autonomic nervous system (ANS) activity. Fifteensubjects inhaled five odorants as olfactory stimuli: lavender(LAV), ethyl acetoacetate (EAA), camphor (CAM), acetic acid(AA) and butyric acid (BA). After inhaling the odorant, subjectswere requested to fill out an 11-point hedonic scale to rateits pleasantness versus unpleasantness. ANS parameters wereas follows: two electrodermal responses, skin potential (SP)and resistance (SR); two thermovascular parameters, skin bloodflow (SBF) and skin temperature (ST); and two cardiorespiratoryparameters: instantaneous respiratory frequency (IRF) and instantaneousheart rate (IHR). Simultaneous recording of six parameters showedthat specific autonomic patterns were associated with each odorant.An analysis of variance made it possible to differentiate amongthe five odorants. Two-by-two odorant comparisons for autonomicresponses using Tukey's HSD multiple comparison test only permitteddifferentiation between pleasant odorants (LAV and EAA) andunpleasant (AA and BA) ones, but camphor was differentiatedfrom both pleasant and unpleasant odorants. Each odorant elicitedresponses in the different parameters, yet subjects respondedthrough their preferential channels; an average of two channelswas used by each subject. These results when compared with thoseobtained with other senses (visual and auditory). did not evidencethe postulated preferential link between olfaction and emotion.A'strong link between hedonics and ANS response could be demonstratedwhen considering each subject and mainly through his/her preferentialchannel(s); conversely a weak correlation (SR duration excepted)was obtained between inter-subjects' hedonic evaluation. Itseems that for a given population the autonomic response reflectthe odor valence only through some parameters related to themain preferential channel(s) and thus the global autonomic patternhas to be considered. Chem. Senses 22: 237248, 1997. 相似文献
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Dittmar Chmelař Adéla Kašíková Petrana Martineková Michal Hájek Miroslav Rozložník Marek Brabec Jana Janečková Jana Vobejdová Ivan Čižnár 《Folia microbiologica》2018,63(3):283-290
The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1–6) and strains isolated from colorectal carcinoma diagnoses (strains 7–8; Wilcoxon non-parametric test p = 0.0132; t test = 0.1110; t test with log-transformed data, p value = 0.0294). 相似文献
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The U3 Promoter and the nef Gene of Simian Immunodeficiency Virus (SIV) smmPBj1.9 Do Not Confer Acute Pathogenicity upon SIVagm 下载免费PDF全文
Stefan Wagener Matthias T. Dittmar Brigitte Beer Renate Knig Roland Plesker Stephen Norley Reinhard Kurth Klaus Cichutek 《Journal of virology》1998,72(4):3446-3450
Two chimeric proviruses comprising the U3 promoter and the nef gene of simian immunodeficiency virus (SIV) smmPBj1.9 in addition to other genomic regions of SIVagm3mc from African green monkeys (Cercopithecus aethiops) were constructed. The derived chimeric viruses (SIVagm3mc/SIVsmmPBj1.9) were both able to replicate in nonstimulated peripheral blood leukocytes from pig-tailed macaques (Macaca nemestrina), a biological property often correlated with acute pathogenicity. However, only one of the chimeric viruses was acutely pathogenic, inducing a rapid depletion of the peripheral CD4+ T cells in two infected pig-tailed macaques within 10 days after infection in a manner similar to infection with SIVsmmPBj1.9 itself. The other chimeric virus actively replicated during the first 8 weeks after experimental infection of two pig-tailed macaques but induced neither acute disease nor CD4+ T-cell depletion for 113 weeks after infection. Thus, the U3 promoter and the nef gene of SIVsmmPBj1.9 alone appear to be insufficient to confer acute pathogenicity to SIVagm3mc. 相似文献