全文获取类型
收费全文 | 9064篇 |
免费 | 939篇 |
国内免费 | 3篇 |
出版年
2023年 | 33篇 |
2022年 | 30篇 |
2021年 | 137篇 |
2020年 | 98篇 |
2019年 | 98篇 |
2018年 | 128篇 |
2017年 | 142篇 |
2016年 | 198篇 |
2015年 | 340篇 |
2014年 | 411篇 |
2013年 | 523篇 |
2012年 | 598篇 |
2011年 | 618篇 |
2010年 | 405篇 |
2009年 | 374篇 |
2008年 | 531篇 |
2007年 | 530篇 |
2006年 | 495篇 |
2005年 | 453篇 |
2004年 | 432篇 |
2003年 | 391篇 |
2002年 | 375篇 |
2001年 | 168篇 |
2000年 | 153篇 |
1999年 | 150篇 |
1998年 | 128篇 |
1997年 | 82篇 |
1996年 | 104篇 |
1995年 | 92篇 |
1994年 | 85篇 |
1993年 | 78篇 |
1992年 | 95篇 |
1991年 | 92篇 |
1990年 | 93篇 |
1989年 | 80篇 |
1988年 | 64篇 |
1987年 | 94篇 |
1986年 | 59篇 |
1985年 | 70篇 |
1984年 | 70篇 |
1983年 | 78篇 |
1982年 | 56篇 |
1981年 | 54篇 |
1980年 | 36篇 |
1979年 | 58篇 |
1978年 | 52篇 |
1977年 | 58篇 |
1976年 | 49篇 |
1974年 | 51篇 |
1972年 | 37篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Fate of corticotrophins in an isolated adrenal-cell bioassay and decrease of peptide breakdown by cell purification 总被引:5,自引:3,他引:2 下载免费PDF全文
Hugh P. J. Bennett Gillian Bullock P. J. Lowry Colin McMartin Judith Peters 《The Biochemical journal》1974,138(2):185-194
1. The fate of corticotrophins in a trypsin-dispersed rat adrenal-cell assay system was investigated with a view to establishing whether differences in the rate of inactivation might contribute to potency differences observed between analogues. 2. Corticotrophin-(1-24)-tetracosapeptide and to a lesser extent synthetic 1-39 corticotrophins were found to be inactivated during incubation with cell suspension. 3. Peptide fragments were isolated by using [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide as a marker. The fragments indicate a peptidase with a predominantly tryptic specificity. 4. The peptidase is present in the extracellular fluid and is released from cells when they are damaged. 5. Cells were fractionated on an albumin gradient. Cells from the zona fasciculata and the zona intermedia or reticularis were present in fractions which produced fluorogenic steroids in response to corticotrophin. 6. Purification of the cells by centrifugation through albumin decreased degradation by peptidases, so that if the assay is carried out with a dilute suspension of purified cells peptide breakdown should not affect the observed potencies of adrenocorticotrophin analogues. 7. No binding of [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide to cells could be detected at low concentrations of the peptide. This indicated that less than 120 receptors/cell are occupied during stimulation by a dose that would elicit approx. 80% of the maximal response. 相似文献
92.
93.
94.
95.
96.
97.
W. P. Peters 《Genetica》1964,34(1):211-227
Krixousacorixa femorata (Guérin), a Mexican corixid, has symmetrical females and asymmetrical dextral or sinistral males. In studying the genetics of this species a sequence of experimental matings is described and the segregation ratios of the progenies analyzed. On the basis of these experiments a hypothesis, adequately explaining the data, is proposed.Exhypothesi the inheritance of the polymorphic characteristic of asymmetry depends upon the allelomorphic series of three genes,D, D
8, andd, of whichD (dextrality) is dominant toD
s
(sinistrality) which in turn is dominant tod (dextrality). The intermediate member of the series is lethal as a homozygote.A portion of the dissertation submitted in partial fulfillment of the requirements for the doctorate at Cambridge University. I wish to thank Professors SirRonald Fisher of Cambridge University andG. E. Hutchinson of Yale University for their kind help. 相似文献
98.
99.
1. Chondromucoprotein from bovine nasal cartilage was purified by cetylpyridinium chloride or by bismuth nitrate in acetone. 2. Amino acid compositions of crude and purified preparations were compared and few differences were found, in spite of the decrease in protein content on purification. 3. Amino acid analysis of bismuth-purified material revealed the existence of four groups of amino acids. Within each group, the amino acids were present in approximately equimolar concentrations. 4. Amino end-group assay on the same material showed six alpha-DNP derivatives. 5. A molecular weight of 6.3x10(5) for the protein-polysaccharide complex was calculated from the latter analysis. 相似文献
100.
1. The metabolism of fluoride in seedlings and small plants of Acacia georginae has been studied with the idea of finding the conditions under which the plant makes fluoroacetate in the laboratory. 2. Individual seedlings vary in the extent to which they take up fluoride and convert it into a form other than inorganic which is here called `organic' fluoride, F(org.). The differences between the toxicity of A. georginae Gidyea trees may therefore be genetic in origin. 3. The uptake of fluoride from solutions 0·525–1·05mm (10–20p.p.m.) was not large. In 1–4 days it reached 8 p.p.m. in the aerial parts and 16 p.p.m. in the roots. Unlike the distribution of the halogen in grass, total fluoride was greater than inorganic fluoride. It was almost a rule that more `organic' fluoride was present in the roots than in the aerial parts. 4. With higher concentrations of fluoride 10·5–15·75mm (200–300p.p.m.) much larger amounts of fluoride were taken up, especially by the roots, and much more apparent organic fluoride was formed. 5. pH had a large influence upon the intake, this being lowest at an initial pH8·4 and highest at pH4·0. The pH outside this range was not investigated. 相似文献