Das endoplasmatische Retikulum subletal bestrahlter Hühnerherzmyoblasten im Dienste des Stofftransportes

Zusammenfassung Durch eine kombinierte elektronenmikroskopisch-histochemische Methode nach Komnick wurden Natrium- und Chloridionen im proximalen Tubulusepithel der Rattenniere dargestellt. Während die als Natriumanimonat gefällten Natriumionen intrazellulär — d. h. intracytoplasmatisch, intramitochondrial, intranukleär — und extracellulär in den zwischenzelligen Faltenräumen vorkommen, beschränkt sich die Lokalisation der als AgCl gefällten Chloridionen weitgehend auf die Räume zwischen den einzelnen Tubulusepithelien.Die Möglichkeit einer passiven interzellulären Diffusion des Chloridions und eines aktiven transzellulären Transports des Natriumions wird diskutiert.

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Summary Sodium and chloride ions are localized in the proximal tubule cells of the rat kidney by a combined electron-microscopic-histochemical method after Komnick. Sodium ions, precipitated as sodium-antimonate, are found in the cytoplasm, the mitochondrial matrix, and the nucleoplasm as well as extracellularly.Chloride ions, precipitated as AgCl, are mainly localized between adjacent tubule cells.The possibility of a passive intercellular diffusion of chloride ions and an active transcellular transport of sodium ions is discussed.

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Auszugsweise vorgetragen auf dem 3. Symposium der Gesellschaft für Nephrologie, Berlin 1964.     

Das piezoelektrische Verhalten der menschlichen Zahnhartgewebe

Zusammenfassung Bei menschlichen Zähnen wurde piezoelektrisches Verhalten festgestellt; dieses ist gebunden an den Kollagenfaseranteil des Dentins und des Zahnzementes. Schmelz ist nicht piezoelektrisch; entmineralisierte Zähne zeigen ein verstärktes piezoelektrisches Verhalten.Es besteht eine piezoelektrische Achse in der physiologischen Zahnlängsrichtung, deren Richtungssinn bei allen Zähnen des Ober- und Unterkiefers gleich ist und offenbar der morphogenetischen Entwicklungsrichtung des Zahnes entspricht. Außerdem besteht rund um die piezoelektrische Längsachse herum in allen radialen Richtungen zwischen Pulpahöhle und Zahnaußenseite piezoelektrisches Verhalten, das möglicherweise in Beziehung zur Struktur der Dentinlamellen und damit zur morphogenetischen Zahnentwicklung in radialer Richtung steht.Die piezoelektrischen Effekte zeigen von Zahn zu Zahn eine erhebliche biologische Streubreite. Bei unserem Untersuchungsmaterial lagen die Effekte zwischen 3,5·10^–10 und 1,9·10^–9 est E Ldg./dyn. Diese Werte entsprechen etwa 0,5 bis 2,8% des piezoelektrischen Koeffizienten d₁₁ von -Quarz.

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Summary Human teeth are piezoelectric. This is due to the collagen-fibril content of the dentine and tooth cement. Enamel is not piezoelectric; decalcified teeth show increased piezoelectric behaviour.There is a piezoelectrical axis corresponding to the physiological longitudinal tooth axis, which is in the same direction in all teeth of both upper and lower jaws, and which seems to correspond to the morphogenetical development axis of teeth. Piezoelectric behaviour also exists between the pulp cavity and outer surface of the tooth in all directions radial to the piezoelectric longitudinal axis. The latter is possibly due to the dentine lamellae, and therefore may be connected with the radial direction of morphogenetical tooth development.The piezoelectric effects show large biological variations in different teeth. The effects from our research materials vary between 3.5·10^–10 and 1.9·10^–9 e.s.u./dynes. These results correspond to about 0.5 to 2.8% of the piezoelectrical coefficient d₁₁ of -quartz.

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Unterstützt durch Sachbeihilfen der Deutschen Forschungsgemeinschaft.

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cand. med. dent. und derzeit Doktorand an der Klinik und Poliklinik für Zahn-, Mund- und Kieferkrankheiten, Universität Kiel.     

Bellevalia ciliata (Cyr.) Nees (Hyacinthaceae) neu für Bulgarien

(Bellevalia ciliata was recorded in north-east Bulgaria south of the Dobrudsha, within field and steppe vegetation. Vegetation records and a distribution map are presented. Based on taxonomic studies it is proposed to combineB. ciliata, B. sarmatica (Pall.) Wor. andB. speciosa Wor. under the oldest nameB. ciliata (Cyr.) Nees.     

Microbial mats and physicochemistry in a saltern in the Bretagne (France) and in a laboratory scale saltern model

Abstract A saltern near La Baule (Bretagne, France) was remodeled in a programmable temperature and humidity controlled walk-in environmental chamber resembling the characteristics of the original saltern. The saltern showed different types of microbial mats predominantly composed of algae, oxy- and anoxyphotobacteria, and associated chemoorganotrophic bacteria, fungi and animals. Well-developed microbial mats were found up to a salinity of 10% during the three or four months in summer when salinity gradients and NaCl precipitation were established. The main phototrophic organisms were diatoms, the cyanobacteria Aphanothece, Microcoleus, Spirulina , and Oscillatoria , and Chromatiaceae. At higher salinity, Halobacterium sp., diatoms, and Dunaliella were dominant. Typical microbial mats and saltern-typical invertebrate, algal and bacterial species also developed in the saltern model, building up a stable community. The ionic composition of the brines and physicochemical parameters were similar to those determined for the original saltern. Different photosynthetic organisms, e.g. a filamentous purple bacterium and a hypersaline Chloroflexus -like organism, could be enriched within the microbial mats by changing the light regime.     

A microdeletion of less than 250 kb, including the proximal part of the FMR-1 gene and the fragile-X site, in a male with the clinical phenotype of fragile-X syndrome

A gene designated "FMR-1" has been isolated at the fragile-X locus. One exon of this gene is carried on a 5.1-kb EcoRI fragment that exhibits length variation in fragile-X patients because of amplification of or insertion into a CGG-repeat sequence. This repeat probably represents the fragile site. The EcoRI fragment also includes an HTF island that is hypermethylated in fragile-X patients showing absence of FMR-1 mRNA. In this paper, we present further evidence that the FMR-1 gene is involved in the clinical manifestation of the fragile-X syndrome and also in the expression of the cellular phenotype. A deletion including the HTF island and exons of the FMR-1 gene was detected in a fragile X-negative mentally retarded male who presented the clinical phenotype of the fragile-X syndrome. The deletion involves less than 250 kb of genomic DNA, including DXS548 and at least five exons of the FMR-1 gene. These data support the hypothesis that loss of function of the FMR-1 gene leads to the clinical phenotype of the fragile-X syndrome. In the fragile-X syndrome, there are pathogenetic mechanisms other than amplification of the CGG repeat that do have the same phenotypic consequences.     

Quenching of Hoechst 33258 fluorescence in erythroid precursors.

Quenching of the fluorescence of DNA-bound Hoechst 33258 in erythroid precursors was studied by flow cytometry and cytochemistry. This quenching artifact may affect the measurement of ploidy in specific cases. The bone marrow cells of two patients with hemolytic disease and active erythropoiesis contained subpopulations of cells with an apparent hypodiploid DNA content as measured by flow cytometry of paraformaldehyde-fixed cells stained with Hoechst 33258. No aneuploidy was detected in either of the two cases when cells were stained with mithramycin or 7-aminoactinomycin D. Cells exhibiting reduced Hoechst 33258 fluorescence expressed glycophorin A and low amounts of CD36, and were therefore erythroid precursors. In one case studied, the number of cells with reduced Hoechst 33258 fluorescence and glycophorin A expressed agreed well with the number of cells containing nuclear hemoglobin. In the other case, hemoglobin was present in a significant proportion of nucleated cells. Calculated values for the efficiency of resonance energy transfer from Hoechst 33258 to hemoglobin were in accordance with the observed levels of quenching (approximately 10%). However, the results could also be explained by hemoglobin reabsorption of Hoechst 33258 fluorescence. Nuclei stained with Hoechst 33258 showed uniform fluorescence, probably due to extraction of hemoglobin during the isolation procedure.     

Prey selection of the shallow water fishPomatoschistus minutus (Gobiidae,Teleostei) in the SW Baltic

The aim of this investigation is to demonstrate the reasons of an opportunist feeder to select some components of the available food supply and to avoid others. Object of this test is the eurytopic and euryhaline sand goby,Pomatoschistus minutus (Pallas), from the Luebeck Bight. It inhabits both sand habitats as well as bottoms mixed with pebbles or continuous hard substrates. Therefore, it is often in close contact withMytilus-belts which present a very rich food supply of small crustaceans. The selectivity behaviour of sand gobies from a sand/clay ecotone was compared with that of populations from a stony pier and a sand bottom during the reproduction period. Regarding the numbers of prey organisms, crustaceans of the periphyton were generally preferred at the pier but avoided in the ecotone. The same is valid for prey organisms of the psammal which were preferred by gobies of the sand bottom but mostly avoided by populations of mixed bottoms. Analyses of size selections revealed that the preferred gammarids orJaera isopods were between 2 and 5 mm length. A balanced relation of goby biomass and utilizable food supply (predatory impact index) seems to effect positive selectivity of gammarids in theMytilus-belts or of harpacticoids in the sand bottom.