全文获取类型
收费全文 | 3524篇 |
免费 | 303篇 |
国内免费 | 2篇 |
出版年
2024年 | 5篇 |
2023年 | 42篇 |
2022年 | 57篇 |
2021年 | 155篇 |
2020年 | 93篇 |
2019年 | 125篇 |
2018年 | 129篇 |
2017年 | 121篇 |
2016年 | 180篇 |
2015年 | 228篇 |
2014年 | 232篇 |
2013年 | 283篇 |
2012年 | 310篇 |
2011年 | 334篇 |
2010年 | 194篇 |
2009年 | 146篇 |
2008年 | 189篇 |
2007年 | 210篇 |
2006年 | 154篇 |
2005年 | 119篇 |
2004年 | 133篇 |
2003年 | 107篇 |
2002年 | 90篇 |
2001年 | 22篇 |
2000年 | 13篇 |
1999年 | 18篇 |
1998年 | 19篇 |
1997年 | 9篇 |
1996年 | 18篇 |
1995年 | 10篇 |
1994年 | 7篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 8篇 |
1990年 | 5篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有3829条查询结果,搜索用时 15 毫秒
21.
22.
Carlos F. de Mello Diego D. De La Vega Leandro T. Pizutti Fabiane P. Lopes Maribel A. Rubin Jaime G. Homerich Dr. Carlos R. Melo Joana E. Somer Diogo O. Souza Moacir Wajner 《Neurochemical research》1995,20(12):1437-1441
The effect ofl-pyroglutamic acid, a metabolite that accumulates in pyroglutamic aciduria, on different neurochemical parameters was investigated in adult male Wistar rats. Glutamate binding, adenylate cyclase activity and G protein coupling to adenylate cyclase were assayed in the presence of the acid.l-pyroglutamic acid decreased Na+-dependent and Na+-independent glutamate binding Basal and GMP-PNP stimulated adenylate cyclase activity were not affected by the acid. Furthermore, rats received unilateral intrastriatal injections of 10–300 nmol of bufferedl-pyroglutamic acid. Vehicle (0.25 M Tris-Cl, pH 7.35–7.4) was injected into the contralateral striatum. Neurotoxic damage was assessed seven days after the injection by histological examination and by weighing both cerebral hemispheres. No difference in histology or weight could be identified between hemispheres. These results suggest that, although capable of interfering with glutamate binding, pyroglutamate did not cause a major lesion in the present model of neurotoxicity. 相似文献
23.
24.
Nader Morshed William T Ralvenius Alexi Nott L Ashley Watson Felicia H Rodriguez Leyla A Akay Brian A Joughin PingChieh Pao Jay Penney Lauren LaRocque Diego Mastroeni LiHuei Tsai Forest M White 《Molecular systems biology》2020,16(12)
Alzheimer’s disease (AD) is characterized by the appearance of amyloid‐β plaques, neurofibrillary tangles, and inflammation in brain regions involved in memory. Using mass spectrometry, we have quantified the phosphoproteome of the CK‐p25, 5XFAD, and Tau P301S mouse models of neurodegeneration. We identified a shared response involving Siglec‐F which was upregulated on a subset of reactive microglia. The human paralog Siglec‐8 was also upregulated on microglia in AD. Siglec‐F and Siglec‐8 were upregulated following microglial activation with interferon gamma (IFNγ) in BV‐2 cell line and human stem cell‐derived microglia models. Siglec‐F overexpression activates an endocytic and pyroptotic inflammatory response in BV‐2 cells, dependent on its sialic acid substrates and immunoreceptor tyrosine‐based inhibition motif (ITIM) phosphorylation sites. Related human Siglecs induced a similar response in BV‐2 cells. Collectively, our results point to an important role for mouse Siglec‐F and human Siglec‐8 in regulating microglial activation during neurodegeneration. 相似文献
25.
26.
Jean Armengaud Agnès Delaunay-Moisan Jean-Yves Thuret Eelco van Anken Diego Acosta-Alvear Tomás Aragón Carolina Arias Marc Blondel Ineke Braakman Jean-François Collet René Courcol Antoine Danchin Jean-François Deleuze Jean-Philippe Lavigne Sophie Lucas Thomas Michiels Edward R. B. Moore Jonathon Nixon-Abell Ramon Rossello-Mora Zheng-Li Shi Antonio G. Siccardi Roberto Sitia Daniel Tillett Kenneth N. Timmis Michel B. Toledano Peter van der Sluijs Elisa Vicenzi 《Environmental microbiology》2020,22(6):1997-2000
The current SARS-CoV-2 pandemic is wreaking havoc throughout the world and has rapidly become a global health emergency. A central question concerning COVID-19 is why some individuals become sick and others not. Many have pointed already at variation in risk factors between individuals. However, the variable outcome of SARS-CoV-2 infections may, at least in part, be due also to differences between the viral subspecies with which individuals are infected. A more pertinent question is how we are to overcome the current pandemic. A vaccine against SARS-CoV-2 would offer significant relief, although vaccine developers have warned that design, testing and production of vaccines may take a year if not longer. Vaccines are based on a handful of different designs (i), but the earliest vaccines were based on the live, attenuated virus. As has been the case for other viruses during earlier pandemics, SARS-CoV-2 will mutate and may naturally attenuate over time (ii). What makes the current pandemic unique is that, thanks to state-of-the-art nucleic acid sequencing technologies, we can follow in detail how SARS-CoV-2 evolves while it spreads. We argue that knowledge of naturally emerging attenuated SARS-CoV-2 variants across the globe should be of key interest in our fight against the pandemic. 相似文献
27.
Gemma Aragonès Kalavathi Dasuri Opeoluwa Olukorede Sarah G. Francisco Carol Renneburg Caroline Kumsta Malene Hansen Shun Kageyama Masaaki Komatsu Sheldon Rowan Jonathan Volkin Michael Workman Wenxin Yang Paula Daza Diego Ruano Helena Dominguez‐Martín José Antonio Rodríguez‐Navarro Xue‐Liang Du Michael A. Brownlee Eloy Bejarano Allen Taylor 《Aging cell》2020,19(11)
28.
Olga P. Nyssen Angeles Perez‐Aisa Luis Rodrigo Manuel Castro Pilar Mata Romero Juan Ortuo Jesus Barrio Jose Maria Huguet Ines Modollel Noelia Alcaide Alfredo Lucendo Xavier Calvet Monica Perona Barbara Gomez Blas Jose Gomez Rodriguez Pilar Varela Manuel Jimenez‐Moreno Manuel Dominguez‐Cajal Liliana Pozzati Diego Burgos Luis Bujanda Jenifer Hinojosa Javier Molina‐Infante Tommaso Di Maira Luis Ferrer Luis Fernndez‐Salazar Ariadna Figuerola Llucia Tito Cristobal de la Coba Judith Gomez‐Camarero Nuria Fernandez Maria Caldas Ana Garre Elena Resina Ignasi Puig Colm OMorain Francis Megraud Javier P. Gisbert 《Helicobacter》2020,25(5)
29.
Monja-Mio Kelly M. Olvera-Casanova Diego Herrera-Herrera Gaston Herrera-Alamillo Miguel Ángel Sánchez-Teyer Felipe L. Robert Manuel L. 《In vitro cellular & developmental biology. Plant》2020,56(5):662-669
In Vitro Cellular & Developmental Biology - Plant - 相似文献
30.
Pedro Len‐Lobos Marcela A. Bustamante‐Snchez Cara R. Nelson Diego Alarcn Rodrigo Hasbún Michael Way Hugh W. Pritchard Juan J. Armesto 《Restoration Ecology》2020,28(2):277-281
We argue that the need for a quality seed supply chain is a major bottleneck for the restoration of Chile's native ecosystems, thus supplementing the list of bottlenecks proposed by Bannister et al. in 2018. Specifically, there is a need for defining seed transfer zones, developing standards and capacities for properly collecting and storing seeds, reducing information gaps on seed physiology and longevity, and implementing an efficient seed supply chain with certification of seed origin and quality. Without such capacities, countries are unlikely to meet their restoration commitments. Although we focus on bottlenecks in Chile, the issues we raise are relevant to other countries and thus the global agenda for ecological restoration. 相似文献