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91.
Berckmans RJ Nieuwland R Kraan MC Schaap MC Pots D Smeets TJ Sturk A Tak PP 《Arthritis research & therapy》2005,7(3):R536-R544
Synovial fluid from patients with various arthritides contains procoagulant, cell-derived microparticles. Here we studied
whether synovial microparticles modulate the release of chemokines and cytokines by fibroblast-like synoviocytes (FLS). Microparticles,
isolated from the synovial fluid of rheumatoid arthritis (RA) and arthritis control (AC) patients (n = 8 and n = 3, respectively), were identified and quantified by flow cytometry. Simultaneously, arthroscopically guided synovial biopsies
were taken from the same knee joint as the synovial fluid. FLS were isolated, cultured, and incubated for 24 hours in the
absence or presence of autologous microparticles. Subsequently, cell-free culture supernatants were collected and concentrations
of monocyte chemoattractant protein-1 (MCP-1), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor (GM-CSF), vascular
endothelial growth factor (VEGF) and intracellular adhesion molecule-1 (ICAM-1) were determined. Results were consistent with
previous observations: synovial fluid from all RA as well as AC patients contained microparticles of monocytic and granulocytic
origin. Incubation with autologous microparticles increased the levels of MCP-1, IL-8 and RANTES in 6 of 11 cultures of FLS,
and IL-6, ICAM-1 and VEGF in 10 cultures. Total numbers of microparticles were correlated with the IL-8 (r = 0.91, P < 0.0001) and MCP-1 concentrations (r = 0.81, P < 0.0001), as did the numbers of granulocyte-derived microparticles (r = 0.89, P < 0.0001 and r = 0.93, P < 0.0001, respectively). In contrast, GM-CSF levels were decreased. These results demonstrate that microparticles might modulate
the release of chemokines and cytokines by FLS and might therefore have a function in synovial inflammation and angiogenesis. 相似文献
92.
A dual role for Integrin α6β4 in modulating hereditary neuropathy with liability to pressure palsies 下载免费PDF全文
Yannick Poitelon Vittoria Matafora Nicholas Silvestri Desirée Zambroni Claire McGarry Nora Serghany Thomas Rush Domenica Vizzuso Felipe A. Court Angela Bachi Lawrence Wrabetz Maria Laura Feltri 《Journal of neurochemistry》2018,145(3):245-257
93.
Vivien Krell Desirée Jakobs-Schoenwandt Stefan Vidal Anant V. Patel 《Fungal biology》2018,122(5):373-378
The recent discovery that entomopathogenic fungi can grow endophytically in plant tissues has spurred research into novel plant protection measures. However, current applications of fungi aiming at endophytism mostly lack targeted formulation strategies resulting in low efficacy. Here, we aimed at enhancing Metarhizium brunneum CB15 endophytism in potato plants by (i) improvement of fungal growth from beads and (ii) cellulase formation or addition to encapsulated mycelium. We found that beads supplemented with cellulose alone or in addition with inactivated baker's yeast exhibited cellulase activity and increased mycelial growth by 12.6 % and 13.6 %, respectively. Higher enzymatic activity achieved by cellulase co-encapsulation promoted a shift from mycelial growth to spore formation with maximum numbers of 2.5 × 108 ± 6.1 × 107 per bead. This correlated with improved endophytism in potato plants by 61.2 % compared to non-supplemented beads. Our study provides first evidence that customized formulations of fungal entomopathogens with enzymes can improve endophytism and this may increase efficacy in plant protection strategies against herbivorous pests. 相似文献
94.
Addressing the Life Cycle of Sewers in Contrasting Cities through an Eco‐Efficiency Approach 下载免费PDF全文
Anna Petit‐Boix Carla Arnal Desirée Marín Alejandro Josa Xavier Gabarrell Joan Rieradevall 《Journal of Industrial Ecology》2018,22(5):1092-1104
Evaluating the sustainability of the urban water cycle is not straightforward, although a variety of methods have been proposed. Given the lack of integrated data about sewers, we applied the eco‐efficiency approach to two case studies located in Spain with contrasting climate, population, and urban and sewer configurations. Our goal was to determine critical variables and life cycle stages and provide results for decision making. We used life cycle assessment and life cycle costing to evaluate their environmental and economic impacts. Results showed that both cities have a similar profile, albeit their contrasting features, that is, operation and maintenance, was the main environmental issue (50% to 70% of the impacts) and pipe installation registered the greatest economic capital expenditure (70% to 75%) due to labor. The location of the wastewater treatment plant (WWTP) is an essential factor in our analysis mainly due to the topography effects (e.g., the annual pump energy was 13 times greater in Calafell). Using the eco‐efficiency portfolio, we observed that sewers might be less eco‐efficient than WWTPs and that we need to envision their design in the context of an integrated WWTP‐sewer management to improve sewer performance. In terms of methodological approach, the bidimensional nature of eco‐efficiency enables the benchmarking of product systems and might be more easily interpreted by the general public. However, there are still some constraints that should be addressed to improve communication, such as the selection of indicators discussed in the article. 相似文献
95.
Yoony YJ Gent Karin Weijers Carla FM Molthoff Albert D Windhorst Marc C Huisman Desirée EC Smith Sumith A Kularatne Gerrit Jansen Philip S Low Adriaan A Lammertsma Conny J van der Laken 《Arthritis research & therapy》2013,15(2):R37
Introduction
Detection of (subclinical) synovitis is relevant for both early diagnosis and monitoring of therapy of rheumatoid arthritis (RA). Previously, the potential of imaging (sub)clinical arthritis was demonstrated by targeting the translocator protein in activated macrophages using (R)-[11C]PK11195 and positron emission tomography (PET). Images, however, also showed significant peri-articular background activity. The folate receptor (FR)-β is a potential alternative target for imaging activated macrophages. Therefore, the PET tracer [18F]fluoro-PEG-folate was synthesized and evaluated in both in vitro and ex vivo studies using a methylated BSA induced arthritis model.Methods
[18F]fluoro-PEG-folate was synthesized in a two-step procedure. Relative binding affinities of non-radioactive fluoro-PEG-folate, folic acid and naturally circulating 5-methyltetrahydrofolate (5-Me-THF) to FR were determined using KB cells with high expression of FR. Both in vivo [18F]fluoro-PEG-folate PET and ex vivo tissue distribution studies were performed in arthritic and normal rats and results were compared with those of the established macrophage tracer (R)-[11C]PK11195.Results
[18F]fluoro-PEG-folate was synthesized with a purity >97%, a yield of 300 to 1,700 MBq and a specific activity between 40 and 70 GBq/µmol. Relative in vitro binding affinity for FR of F-PEG-folate was 1.8-fold lower than that of folic acid, but 3-fold higher than that of 5-Me-THF. In the rat model, [18F]fluoro-PEG-folate uptake in arthritic knees was increased compared with both contralateral knees and knees of normal rats. Uptake in arthritic knees could be blocked by an excess of glucosamine-folate, consistent with [18F]fluoro-PEG-folate being specifically bound to FR. Arthritic knee-to-bone and arthritic knee-to-blood ratios of [18F]fluoro-PEG-folate were increased compared with those of (R)-[11C]PK11195. Reduction of 5-Me-THF levels in rat plasma to those mimicking human levels increased absolute [18F]fluoro-PEG-folate uptake in arthritic joints, but without improving target-to-background ratios.Conclusions
The novel PET tracer [18F]fluoro-PEG-folate, designed to target FR on activated macrophages provided improved contrast in a rat model of arthritis compared with the accepted macrophage tracer (R)-[11C]PK11195. These results warrant further exploration of [18F]fluoro-PEG-folate as a putative PET tracer for imaging (sub)clinical arthritis in RA patients. 相似文献96.
Adam Mitchell Weihua Guan Rodney Staggs Aimee Hamel Sameh Hozayen Neeta Adhikari Suzanne Grindle Snider Desir Ranjit John Jennifer L. Hall Peter Eckman 《PloS one》2013,8(10)
Introduction
Continuous-flow left ventricular assist devices (LVADs) are an established therapy for patients with end-stage heart failure. The short- and long-term impact of these devices on peripheral blood gene expression has not been characterized, and may provide insight into the molecular pathways mediated in response to left ventricular remodeling and an improvement in overall systemic circulation. We performed RNA sequencing to identify genes and pathways influenced by these devices.Methods
RNA was extracted from blood of 9 heart failure patients (8 male) prior to LVAD implantation, and at 7 and 180 days postoperatively. Libraries were sequenced on an Illumina HiSeq2000 and sequences mapped to the human Ensembl GRCh37.67 genome assembly.Results
A specific set of genes involved in regulating cellular immune response, antigen presentation, and T cell activation and survival were down-regulated 7 days after LVAD placement. 6 months following LVAD placement, the expression levels of these genes were significantly increased; yet importantly, remained significantly lower than age and sex-matched samples from healthy controls.Conclusions
In summary, this genomic analysis identified a significant decrease in the expression of genes that promote a healthy immune response in patients with heart failure that was partially restored 6 months following LVAD implant. 相似文献97.
Beta1 integrin activates Rac1 in Schwann cells to generate radial lamellae during axonal sorting and myelination 下载免费PDF全文
Nodari A Zambroni D Quattrini A Court FA D'Urso A Recchia A Tybulewicz VL Wrabetz L Feltri ML 《The Journal of cell biology》2007,177(6):1063-1075
Myelin is a multispiraled extension of glial membrane that surrounds axons. How glia extend a surface many-fold larger than their body is poorly understood. Schwann cells are peripheral glia and insert radial cytoplasmic extensions into bundles of axons to sort, ensheath, and myelinate them. Laminins and beta1 integrins are required for axonal sorting, but the downstream signals are largely unknown. We show that Schwann cells devoid of beta1 integrin migrate to and elongate on axons but cannot extend radial lamellae of cytoplasm, similar to cells with low Rac1 activation. Accordingly, active Rac1 is decreased in beta1 integrin-null nerves, inhibiting Rac1 activity decreases radial lamellae in Schwann cells, and ablating Rac1 in Schwann cells of transgenic mice delays axonal sorting and impairs myelination. Finally, expressing active Rac1 in beta1 integrin-null nerves improves sorting. Thus, increased activation of Rac1 by beta1 integrins allows Schwann cells to switch from migration/elongation to the extension of radial membranes required for axonal sorting and myelination. 相似文献
98.
Regulation of insulin secretion and GLUT4 trafficking by the calcium sensor synaptotagmin VII 总被引:2,自引:0,他引:2
Li Y Wang P Xu J Gorelick F Yamazaki H Andrews N Desir GV 《Biochemical and biophysical research communications》2007,362(3):658-664
Insulin regulates blood glucose by promoting uptake by fat and muscle, and inhibiting production by liver. Insulin-stimulated glucose uptake is mediated by GLUT4, which translocates from an intracellular compartment to the plasma membrane. GLUT4 traffic and insulin secretion both rely on calcium-dependent, regulated exocytosis. Deletion of the voltage-gated potassium channel Kv1.3 results in constitutive expression of GLUT4 at the plasma membrane. Inhibition of channel activity stimulated GLUT4 translocation through a calcium dependent mechanism. The synaptotagmins (Syt) are calcium sensors for vesicular traffic, and Syt VII mediates lysosomal and secretory granule exocytosis. We asked if Syt VII regulates insulin secretion by pancreatic beta cells, and GLUT4 translocation in insulin-sensitive tissues mouse model. Syt VII deletion (Syt VII -/-) results in glucose intolerance and a marked decrease in glucose-stimulated insulin secretion in vivo. Pancreatic islet cells isolated from Syt VII -/- cells secreted significantly less insulin than islets of littermate controls. Syt VII deletion disrupted GLUT4 traffic as evidenced by constitutive expression of GLUT4 present at the plasma membrane of fat and skeletal muscle cells and unresponsiveness to insulin. These data document a key role for Syt VII in peripheral glucose homeostasis through its action on both insulin secretion and GLUT4 traffic. 相似文献
99.
Dams are known to impact river channels and ecosystems, both during their lifetime and in their decommissioning. In this study, we applied a before-after-control-impact design associated with two small dam removals to investigate abiotic and biotic recovery trajectories from both the elimination of the press disturbance associated with the presence of dams and the introduction of a pulse disturbance associated with removal of dams. The two case studies represent different geomorphic and ecological conditions that we expected to represent low and high sensitivities to the pulse disturbance of dam removal: the 4 m tall, gravel-filled Brownsville Dam on the wadeable Calapooia River and the 12.5 m tall, sand and gravel-filled Savage Rapids Dam on the largely non-wadeable Rogue River. We evaluated both geomorphic and ecological responses annually for two years post removal, and asked if functional traits of the macroinvertebrate assemblages provided more persistent signals of ecological disturbance than taxonomically defined assemblages over the period of study. Results indicate that: 1) the presence of the dams constituted a strong ecological press disturbance to the near-downstream reaches on both rivers, despite the fact that both rivers passed unregulated flow and sediment during the high flow season; 2) ecological recovery from this press disturbance occurred within the year following the restoration action of dam removal, whereas signals of geomorphic disturbance from the pulse of released sediment persisted two years post-removal, and 3) the strength of the press disturbance and the rapid ecological recovery were detected regardless of whether recovery was assessed by taxonomic or functional assemblages and for both case studies, in spite of their different geomorphic settings. 相似文献
100.