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951.
Ronald H. Gray David Serwadda Aaron A. R. Tobian Michael Z. Chen Frederick Makumbi Tara Suntoke Godfrey Kigozi Fred Nalugoda Boaz Iga Thomas C. Quinn Lawrence H. Moulton Oliver Laeyendecker Steven J. Reynolds Xiangrong Kong Maria J. Wawer 《PLoS medicine》2009,6(11)
Background
Randomized trials show that male circumcision (MC) reduces the incidence of HIV and herpes simplex virus type 2 (HSV-2) infections, and symptomatic genital ulcer disease (GUD). We assessed the role of GUD and HSV-2 in the protection against HIV afforded by MC.Methods and Findings
HIV-uninfected men were randomized to immediate (n = 2,756) or delayed MC (n = 2,775) in two randomized trials in Rakai, Uganda. GUD symptoms, HSV-2 status, and HIV acquisition were determined at enrollment and at 6, 12, and 24 mo of follow up. Ulcer etiology was assessed by PCR. We estimated the prevalence and prevalence risk ratios (PRRs) of GUD in circumcised versus uncircumcised men and assessed the effects of HSV-2 serostatus as a risk-modifying factor for GUD. We estimated the proportion of the effect of MC on HIV acquisition that was mediated by symptomatic GUD, and by HSV-2 infection. Circumcision significantly reduced symptomatic GUD in HSV-2-seronegative men (PRR = 0.51, 95% [confidence interval] CI 0.43–0.74), HSV-2-seropositive men (PRR = 0.66, 95% CI 0.51–0.69), and in HSV-2 seroconverters (PRR = 0.48, 95% CI 0.30–0.79). The proportion of acute ulcers due to HSV-2 detected by PCR was 48.0% in circumcised men and 39.3% in uncircumcised men (χ2 p = 0.62). Circumcision reduced the risk of HIV acquisition in HSV-2 seronegative men (incidence rate ratio [IRR] = 0.34, 95% CI 0.15–0.81), and potentially in HSV-2 seroconverters (IRR = 0.56, 95% CI 0.19–1.57; not significant), but not in men with prevalent HSV-2 at enrollment (IRR = 0.89, 95% CI 0.49–1.60). The proportion of reduced HIV acquisition in circumcised men mediated by reductions in symptomatic GUD was 11.2% (95% CI 5.0–38.0), and the proportion mediated by reduced HSV-2 incidence was 8.6% (95% CI −1.2 to 77.1).Conclusions
Circumcision reduced GUD irrespective of HSV-2 status, but this reduction played only a modest role in the protective effect of circumcision on HIV acquisition.NIH Trial registration
ClinicalTrials.gov NCT00425984Gates Foundation Trial registration
ClinicalTrials.gov Please see later in the article for the Editors'' Summary NCT00124878相似文献952.
Woo-Jin Kim Young-Ok Kim Bo-Hye Nam Hee Jeong Kong Eun-Mi Park Jung-Ha Kang Jeong-Ho Lee Kyung-Kil Kim 《Conservation Genetics》2009,10(4):1105-1111
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite
markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from
a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci
were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null
alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree
tracing and constructing a linkage map for olive flounder. 相似文献
953.
Microbial eukaryotic community (0.8–20 μm) composition and its seasonal variation were investigated in large, shallow, subtropical
Lake Taihu located in Southeast China. The water samples were collected monthly from August 2006 to July 2007 at two sites
distantly located in Meiliang Bay and Lake Center, which differed strongly in their trophic status and sediment resuspension.
The microbial eukaryotic community compositions (MECC) were measured using denaturing gradient gel electrophoresis (DGGE)
of PCR-amplified eukaryotic 18S rRNA gene fragments. Seasonal variations were related to environmental factors by means of
redundancy analysis (RDA) in order to reveal the most important regulating factors. At both sites, the structures and Shannon–Wiener
indices of the microbial eukaryotic communities displayed apparent seasonal variations, which were significantly related to
environmental variables. There were significant intrahabitat differences in environmental factors regulating MECC, as well
as in the seasonal dynamics of the two sites during the study period. At Meiliang Bay, the variations of total phosphorus
concentration and cladocera abundance had the most profound impacts on the microbial eukaryotic community composition, while
at Lake Center, the total nitrogen concentration and conductivity were most influential. Our results suggest that the DGGE
method is a cost-effective way to analyze the seasonal dynamics of MECC and their interaction with environmental variables,
which will provide new insights into the diversity and dynamics of MECC. 相似文献
954.
Genetic variation and evolutionary demography of the shrimp Fenneropenaeus chinensis were investigated using sequence data of the complete mitochondrial control region (CR). Fragments of 993 bp of the CR were sequenced for 93 individuals from five localities over most of the species' range in the Yellow Sea and the Bohai Sea. There were 84 variable sites defining 68 haplotypes. Haplotype diversity levels were very high (0.95 ± 0.03-0.99 ± 0.02) in F. chinensis populations, whereas those of nucleotide diversity were moderate to low (0.66 ± 0.36%-0.84 ± 0.46%). Analysis of molecular variance and conventional population statistics (F(ST) ) revealed no significant genetic structure throughout the range of F. chinensis. Mismatch distribution, estimates of population parameters and neutrality tests revealed that the significant fluctuations and shallow coalescence of mtDNA genealogies observed were coincident with estimated demographic parameters and neutrality tests, in implying important past-population size fluctuations or range expansion. Isolation with Migration (IM) coalescence results suggest that F. chinensis, distributed along the coasts of northern China and the Korean Peninsula (about 1000 km apart), diverged recently, the estimated time-split being 12,800 (7,400-18,600) years ago. 相似文献
955.
Changzhong Li Yilin Hu Jian Liang Yawei Kong Jing Huang Qiaoli Feng Shuo Li Guiyou Zhang Liping Xie Rongqing Zhang 《Marine biotechnology (New York, N.Y.)》2010,12(1):100-110
Calcineurin (CN) is a multifunctional protein involved in many important physiological processes in mammalians, but the function
of CN in mollusks is still largely unknown. In the present study, through the shell regeneration system, the changes of enzymatic
activity of CN were determined in the process of shell regeneration in pearl oyster Pinctada fucata. CN was activated immediately and continuously in the shell regeneration process. The speed of shell regeneration was measured
and the ultrastructure of inner shell surface was observed by scanning electron microscopy after inhibiting CN by intramuscular
injection of immunosuppresant cyclosporine A (CsA). The results showed that the speed of shell regeneration was delayed and
the morphology of calcite and aragonite in the inner shell surface became abnormal when CN was inhibited by CsA. Meanwhile,
RT-PCR analysis revealed that the expression of P. fucata BMP-2 in mantle tissue decreased with CsA injection. In vitro secretion level of proteoglycans (PGs) in primary cultures
of mantle cells was also decreased when mantle cells were exposed to CsA. Taken together, our results, for the first time,
show that CN is involved in the shell formation through regulating the expression of Pf-BMP-2 in mantle tissue, which controls the secretion of PGs/GAGs of the mantle epithelial cells. 相似文献
956.
Wei Wei Kong Li Ping Zhang Kai Guo Zhao Pu Liu Zhi Min Yang 《Plant biotechnology journal》2010,8(1):88-99
Carbon monoxide (CO) is an endogenous gaseous molecule and regulates a variety of biological processes in animals. However, whether CO regulates nutrient stress responses in plants is largely unknown. In this paper, we described an observation that CO can regulate iron-homeostasis in iron-starved Arabidopsis. Exogenous CO at 50 μ m was able to prevent the iron deficient-induced chlorosis and improve chlorophyll accumulation. Expression of AtIRT1 , AtFRO2 , AtFIT1 and AtFER1 was up-regulated by CO exposure in iron-deficient seedlings. CO-regulated iron homeostasis could also be found in monocot maize and green alga Chlamydomonas reinhardtii . Treatment with external CO increased iron accumulation in iron-deficient Arabidopsis and C. reinhardtii , and restored leaf greening in Maize ys1 and ys3 mutants (defective in Fe uptake). Moreover, endogenous CO level was increased in Arabidopsis under iron-deficiency. Finally, CO exposure induced NO accumulation in root tips. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO may play an important role in improving plant adaptation to iron deficiency or cross-talking with NO under the iron deficiency. 相似文献
957.
958.
Nguyen Ba Kien In-Soo Kong Min-Gyu Lee Joong Kyun Kim 《Journal of industrial microbiology & biotechnology》2010,37(5):521-529
For the commercial production of CoQ10, batch-type fermentations were attempted in a 150-l fermenter using a mutant strain of R. sphaeroides. Optimum temperature and initial aeration rate were found to be 30°C and 2 vvm, respectively. Under optimum fermentation
conditions, the maximum value of specific CoQ10 content was achieved reproducibly as 6.34 mg/g DCW after 24 h, with 3.02 g/l of DCW. During the fermentation, aeration shift
(from the adequate aeration at the early growth phase to the limited aeration in active cellular metabolism) was a key factor
in CoQ10 production for scale-up. A higher value of the specific CoQ10 content (8.12 mg/g DCW) was achieved in fed-batch fermentation and comparable to those produced by the pilot-scale fed-batch
fermentations of A. tumefaciens, which indicated that the mutant strain of R. sphaeroides used in this study was a potential high CoQ10 producer. This is the first detailed study to demonstrate a pilot-scale production of CoQ10 using a mutant strain of R. sphaeroides. 相似文献
959.
Kong SG Suzuki T Tamura K Mochizuki N Hara-Nishimura I Nagatani A 《The Plant journal : for cell and molecular biology》2006,45(6):994-1005
Phototropins 1 and 2 (phot1 and phot2) function as blue light (BL) photoreceptors for phototropism, chloroplast relocation, stomatal opening and leaf flattening in Arabidopsis thaliana. Phototropin consists of two functional domains, the N-terminal photosensory domain and the C-terminal Ser/Thr kinase domain. However, little is known about the signal transduction pathway that links the photoreceptors and the physiological responses downstream of BL perception. To understand the mechanisms by which phot2 initiates these responses, we transformed the phot1phot2 double mutant of Arabidopsis with constructs encoding translationally fused phot2:green fluorescent protein (P2G). P2G was fully functional for the phot2-specific physiological responses in these transgenic plants. It localized strongly to the plasma membrane and weakly to the cytoplasm in the dark. Upon illumination with BL, punctate P2G staining was formed within a few minutes in addition to the constitutive plasma membrane staining. This punctate distribution pattern matched well with that of the Golgi-localized KAM1DeltaC:mRFP. Brefeldin A (BFA), an inhibitor of vesicle trafficking, induced accumulation of P2G around the perinuclear region even in darkness, but the punctate pattern was not observed. After treatment of these cells with BL, P2G exhibited the punctate distribution pattern that matched with that of the Golgi marker. Hence, the light-dependent association of P2G with the Golgi apparatus was BFA-insensitive. A structure/function analysis indicated that the kinase domain was essential for the Golgi localization of phot2. The BL-induced Golgi localization of phot2 may be one of important signaling steps in the phot2 signal transduction pathway. 相似文献
960.
Lee KI Park Y Park SJ Hwang JH Lee SJ Kim GD Park WK Lee S Jeong D Kong JY Kang HK Cho H 《Bioorganic & medicinal chemistry letters》2006,16(3):737-742
A series of dinaphtho[1,2-b;2',3'-d]furan-7,12-dione derivatives were synthesized and evaluated for inhibitory activities against receptor tyrosine kinases. The naphthofuroquinone compounds with dialkylaminoethoxy group at C(5)-position (7, 8, 10, and 11) manifested strong inhibitory activities against epidermal growth factor receptor and vascular endothelial growth factor receptor. Docking study of 11 with EGFR was also performed. 相似文献