Effects of anhydrous benzoylation on staining with a variety of protein end-group methods

Summary The effects of anhydrous benzoylation were examined in connection with the following protein end-group methods: diazosulfanilic acid — Azure A, the Sakaguchi reaction and a fluorescent variant, the phenanthrequinone fluorescent method for arginine, the Millon reaction, the Morel-Sisley reaction, the mercury orange method, the alloxan and ninhydrinShiff methods, the Dansyl-chloride fluorescent method for lysine, the dimethylaminobenz-aldehydenitrite (DMAB) method for tryptophan, and the acid fluorochrome brilliant sulfoflavin used at pH 2.8 as a stain for basic groups. With all of these methods except the DMAB method for tryptophan, selective blockage of cytoplasmic proteins and staining of nuclei was obtained. With the DMAB reaction, the results were reversed: cytoplasmic staining exceeded nuclear staining after benzoylation.     

Mediation of a plant response to malformin by ethylene

Malformin and ethylene stimulate abscission of the primary leaves of Phaseolus aureus Roxb. in the dark, and abscission stimulation by both compounds is inhibited by indeleacetic acid and CO₂. Ethylene production by malformin-treated buds is stimulated within 4 hours. and up to 8 days, after treatment. Malformin-induced growth disturbances in P. vulgaris L. and abscission in P. aureus are considered mediated by ethylene. Although root curvatures of Zea mays L. are induced by both malformin and ethylene, and malformin is inhibited by CO₂, ethylene production is not stimulated by malformin. A role of ethylene in root curvatures induced by malformin is neither proposed nor disproved.     

Survival of micro-organisms in space

Dried suspensions ofPenicillium roqueforti Thom, Coliphage T-1,Bacillus subtilis and tobacco mosaic virus were exposed to space on board the Gemini-IX-A and XII earth satellites and the Agena-VIII space rocket. All micro-organisms tested survived the direct exposure during the Gemini-IX-A experiment. In the Gemini-XII experiment only the T-1 phage survived the direct exposure. The survival was influenced by the suspending medium and depended on the species of the microorganism. After four months of space flight on the Agena-VIII space rocket surviving fractions between 2×10^–3 and 1.0 were found in the unopened flight container. However, micro-organisms exposed on the cover of the container during this period were completely inactivated. Shielding against solar ultraviolet radiation during flight resulted in survival of micro-organisms exceeding to that of the transport controls, and the survival was considered complete.Sterile methylcellulose collection surfaces were exposed to space on board the Gemini-IX-A and XII satellites in an attempt to collect viable micro-organisms in space. None of the collection surfaces yielded viable micro-organisms.     

Inheritance of quantitative expression of erythrocyte glucose-6-phosphate dehydrogenase activity in the Negro—a twin study

Studies have been conducted on eight sets of monozygous and nine sets of dizygous female Negro twins, both members of whom were heterozygous for G-6-PD deficiency. Twins were studied both by assay of erythrocytic G-6-PD activity and by the methemoglobin elution test (MET). The MET is a procedure which identifies histochemically cells with appreciable G-6-PD activity and permits accurate determination of the percentage of such cells in heterozygotes. Monozygous twins showed significantly less within-pair variation than dizygous twins with both the MET and G-6-PD assay.Concerning the significantly greater agreement in MET results in monozygous twins than dizygous twins, our present working hypothesis is that X-chromosomal inactivation in the Negro female is genetically controlled, rather than random. However, certain alternate hypotheses allowing for random X-inactivation have not been excluded; these include somatic cell selection after random X-inactivation, and cell exchange between identical twins in utero/it. Studies in nontwin related heterozygotes now underway should help differentiate among these various possibilities.In addition to the studies on 17 pairs of female twins heterozygous for G-6-PD deficiency, 26 pairs of nondeficient female Negro twins have been studied by G-6-PD assay. Within-pair variation in monozygous twins was significantly less than within-pair variation in dizygous twins in all cases. The genetic influences detected with the G-6-PD assay in the female twins could theoretically be due to nonrandom X-inactivation, to genetically determined quantitative differences in enzyme activity (e.g., isoalleles), or to both. By appropriate calculations, based on the MET results, we have factored out the effects of X-inactivation on overall enzyme activity in the heterozygous deficient twins. After removal of the effect of X-inactivation, monozygous twins heterozygous for enzyme deficiency continue to show significantly less within-pair variation than dizygous twins. This finding indicates significant genetic influences on quantitative G-6-PD activity other than X-inactivation and other than the deficiency allele. This conclusion has been strengthened by studies on male twins where X-inactivation is not present.Supported by USPHS research grants AM-09381, HE-17544, AM-09919, and HE-03341, by USPHS Career Development Award 1-K3-AM-7959 (Dr. Brewer) and by U.S.A.E.C. Contract (11-1)-1552.     

The Influence of Morphology on Prognosis in Acute Leukemia

The presence of definite cytoplasmic granulation in at least some of the malignant cells was used as the sole criterion to separate 156 patients with acute leukemia into two groups: 110 with myeloblastic (AML), and 46 with lymphoblastic or stem cell leukemia (ALL). The median survival from the onset of symptoms in patients with AML was 20 weeks, and those with ALL 37 weeks. The difference in survival in these two groups is much greater for patients under the age of 25 than for those over the age of 25.     

U-21,963, a New Antibiotic: II. Isolation and Characterization

The isolation and characterization of antibiotic U-21,963 are discussed. This compound is a highly unsaturated monobasic acid with the molecular formula C(9)H(7)NO(3). The molecular weight is 177. It is dextrorotatory, [alpha](D) = +138 degrees , and has a pK(a) of 5.1. The ultraviolet absorption spectrum, which showed a maximum at 223 mmu (epsilon = 15,115), indicates unsaturation alpha-beta to the carboxyl group, and the infrared spectrum suggests the presence of an acetylenic group. Explosive decomposition of U-21,963 at 97 C conforms with the latter. U-21,963 is relatively insoluble in water, but readily soluble in ethyl alcohol, acetone, and halogenated hydrocarbons.     

Presymptomatic testing for Huntington's disease

Summary Presymptomatic testing for Huntington's disease (HD) is possible through the use of restriction fragment length polymorphisms (RFLPs) at the closely linked D4S10 locus. Recombination between the HD and D4S10 loci will occur in 4%–5% of meioses, and is a well-recognised complication of predictive testing. Recombination between RFLPs within the D4S10 locus is a rare event and can usually be ignored. We report a case where such an intra-locus recombination frustrated attempts to predict the chance of a high-risk individual inheriting the HD gene.