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21.
In an appropriate ionic environment, the resting potential of canine cardiac purkinje fibers may have either of two value. By changing the external K concentration, [K](0), in small steps, it was shown that, in the low (1 mM) Cl, Na-containing solutions used in this study, the two levels of resting potential could be obtained only within a narrow range of [K](0) values; that range was usually found between 1 and 4 mM. Within the critical [K](0) range the resting potential could be shifted from either level to the other by the application of small current pulses. It was shown that under these conditions the steady-state current- voltage relationship was “N-shaped,” and that a region of both negative slope, and negative chord conductance lay between the two stable zero-current potentials. The negative chord conductance was largely due to inward sodium current, only part of which was sensitive to tetrodotoxin (TTX). Under appropriate conditions, the negative chord conductance could be abolished by several experimental interventions and the membrane potential thereby shifted from the lower to the higher resting level: those interventions which were effective by presumably diminishing the steady-state inward current included reducing the external sodium concentration, adding TTX, or adding lidocaine; those which presumably increased the steady-state outward current included small increases in [K](0), brief depolarizations to around -20 mV, or the addition of acetylcholine chloride. 相似文献
22.
Luís H Franco Pryscilla F Wowk Célio L Silva Ana PF Trombone Arlete AM Coelho-Castelo Constance Oliver Maria C Jamur Edson L Moretto Vânia LD Bonato 《Genetic vaccines and therapy》2008,6(1):1-11
Background
Application of plasmid DNA for immunization of food-producing animals established new standards of food safety. The addition of foreign products e.g. pDNA into the food chain should be carefully examined to ensure that neither livestock animals nor consumers develop unpredicted or undesirable side-effects.Methods
A quantitative real-time PCR (QRTPCR) methodology was developed to study the biodistribution and persistence of plasmid DNA vaccine pDNAX (pVAX-Hsp60 TM814) in mice and beef cattle. The linear quantification range and the sensitivity of the method was found to be 10 – 109 copies per reaction (500 ng/gDNA) and 3 copies per reaction, respectively.Results
Persistence of pDNAX in mice muscle tissue was restricted to injection site and the amount of pDNAX showed delivery formulation dependent (naked pDNA, electroporation, cationic liposome complexes) and mouse age-dependent clearance form injection site but pDNAX was still detectable even after 365 days. The QRTPCR analysis of various muscle tissue samples of vaccinated beef bulls performed 242–292 days after the last revaccination proved that residual pDNAX was found only in the injection site. The highest plasmid levels (up to 290 copies per reaction) were detected in the pDNAX:CDAN/DOPE group similarly to mice model. No pDNA was detected in the samples from distant muscles and draining lymph nodes.Conclusion
Quantitative real-time PCR (QRTPCR) assay was developed to assess the residual pDNA vaccine pVAX-Hsp60 TM814 in mice and beef cattle. In beef cattle, ultra low residual level of pDNA vaccine was only found at the injection site. According to rough estimation, consumption of muscles from the injection site represents almost an undetectable intake of pDNA (400 fg/g muscle tissue) for consumers. Residual plasmid in native state will hardly be found at measurable level following further meat processing. This study brings supportive data for animal and food safety and hence for further approval of pDNA vaccine field trials. 相似文献23.
ALFRED KÖPF NATHAN RANK HEIKKI ROININEN & JORMA TAHVANAINEN 《Ecological Entomology》1997,22(2):176-183
1. Noxious larval secretions of leaf beetles, which repel generalist predators, do not deter specialist syrphid fly predators (genus Parasyrphus ). These flies cause considerable mortality to the beetles, but little is known about their foraging behaviour.
2. Larvae of Parasyrphus nigritarsis were attracted to the volatile larval secretions produced by two prey species Phratora vitellinae and Linaeidea aenea. Parasyrphus nigritarsis feeds on both beetles in nature. Phratora vitellinae feeds on willows and utilizes host plant compounds for secretion production, while the alder-feeding L. aenea produces an autogenous secretion.
3. Fly larvae were strongly attracted to pieces of filter paper treated with larval secretion of the beetles. They attempted to feed on them for up to 7 min, and were equally attracted to the secretions of Ph. vitellinae and L. aenea . Fly larvae were also attracted to pure salicyl aldehyde, the main component of the secretion of Ph. vitellinae .
4. Fly larvae searched extensively for prey on leaves that had been damaged by beetle larvae. They also followed trails made with solutions containing faecal matter of prey larvae. They showed no differential preference for Ph. vitellinae or L. aenea , but always rejected larvae of the non-prey leaf beetle Agelastica alni .
5. Beetle secretions thus play an important, but unexpected, role in the feeding behaviour of P. nigritarsis . This predator uses the beetle secretion to locate its prey. The implications of these results for three trophic level interactions are discussed. 相似文献
2. Larvae of Parasyrphus nigritarsis were attracted to the volatile larval secretions produced by two prey species Phratora vitellinae and Linaeidea aenea. Parasyrphus nigritarsis feeds on both beetles in nature. Phratora vitellinae feeds on willows and utilizes host plant compounds for secretion production, while the alder-feeding L. aenea produces an autogenous secretion.
3. Fly larvae were strongly attracted to pieces of filter paper treated with larval secretion of the beetles. They attempted to feed on them for up to 7 min, and were equally attracted to the secretions of Ph. vitellinae and L. aenea . Fly larvae were also attracted to pure salicyl aldehyde, the main component of the secretion of Ph. vitellinae .
4. Fly larvae searched extensively for prey on leaves that had been damaged by beetle larvae. They also followed trails made with solutions containing faecal matter of prey larvae. They showed no differential preference for Ph. vitellinae or L. aenea , but always rejected larvae of the non-prey leaf beetle Agelastica alni .
5. Beetle secretions thus play an important, but unexpected, role in the feeding behaviour of P. nigritarsis . This predator uses the beetle secretion to locate its prey. The implications of these results for three trophic level interactions are discussed. 相似文献