全文获取类型
收费全文 | 167篇 |
免费 | 11篇 |
国内免费 | 1篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 7篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 7篇 |
2014年 | 5篇 |
2013年 | 10篇 |
2012年 | 13篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 10篇 |
2008年 | 9篇 |
2007年 | 8篇 |
2006年 | 11篇 |
2005年 | 3篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 5篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1977年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有179条查询结果,搜索用时 15 毫秒
51.
AJ Durston 《Current Genomics》2012,13(4):265-266
52.
Bernhard Y Renard Marc Kirchner Hanno Steen Judith AJ Steen Fred A Hamprecht 《BMC bioinformatics》2008,9(1):355
Background
The reliable extraction of features from mass spectra is a fundamental step in the automated analysis of proteomic mass spectrometry (MS) experiments. 相似文献53.
Christa Testerink Paul B Larsen Fionn McLoughlin Dieuwertje van der Does John AJ van Himbergen Teun Munnik 《Plant signaling & behavior》2008,3(9):681-683
Constitutive triple response 1 (CTR1) is a protein kinase that represses plant responses to ethylene. Recently, we have shown that CTR1 function is negatively regulated by the lipid second messenger phosphatidic acid (PA) in vitro.1 PA was shown to inhibit (1) CTR1''s protein kinase activity, (2) the intramolecular interaction between N-terminus and kinase domain, and (3) the interaction of CTR1 with the ethylene receptor ETR1. PA typically accumulates within minutes in response to biotic or abiotic stresses, which are known to induce ethylene formation. Although long-term treatment with ethephon does stimulate PA accumulation, our results show no fast increase in PA in response to ethylene. A speculative model is presented which explains how stress-induced PA formation could switch on downstream ethylene responses via interaction of the lipid with CTR1.Key words: lipid signaling, phosphatidic acid, ethylene, constitutive triple response 1, plant stress signaling, protein kinase, phospholipase D 相似文献
54.
Teresa Romero Fernando Colmenares Filippo Aureli 《International journal of primatology》2008,29(6):1591-1606
Researchers have associated variation in the occurrence of postconflict behaviors with variation in the relationship quality
between involved individuals. Apart from those on the great apes, the vast majority of postconflict studies involved female-bonded
species and focused on the victim. We examined postconflict affiliation involving the aggressor in a non-female-bonded species,
hamadryas baboons (Papio hamadryas), and investigated the influence of relationship quality on the occurrence of 2 types of postconflict affiliative behaviors.
Hamadryas baboons reconciled after a conflict and the highest rate of reconciliation was between one-male unit (OMU) leader
males and their females. Via direct measures of relationship characteristics we also found that partners with higher-quality
relationship, e.g., highly affiliative dyads and allies, showed higher levels of conciliatory tendency than dyads with lower-quality
relationship, e.g., less affiliative dyads and non-allies. We found evidence of postconflict third-party affiliation initiated
by aggressors, but not by third parties. Further, aggressor-initiated affiliation was more likely with individuals of the
same OMU and individuals with which the aggressor maintained a strongly affiliative relationship. Our findings provide further
support for relationship quality as a fundamental underlying factor not only in reconciliation, but also in postconflict affiliations
involving third parties. 相似文献
55.
The utility of the morphological variation of pollen for resolving the evolutionary history of Billia (subfam. Hippocastanoideae,Sapindaceae) 下载免费PDF全文
In this study, we examined the utility of pollen morphology for resolving questions about the evolutionary history of Billia, which is a poorly known genus of Neotropical trees. Billia has been traditionally circumscribed with two species and treated as sister to Aesculus L. However, the number of species in Billia is uncertain, because the genus exhibits abundant morphological diversity but little discontinuous variation. Therefore, Billia may be monotypic and highly polymorphic, or it may have two species with blurred boundaries due to incipient speciation and/or hybridization. Moreover, one recent molecular phylogenetic study shows Billia nested withinAesculus. Our work sought to address the following questions: (i) Are there discontinuities in the pollen of Billia that may suggest species boundaries? (ii) Does the pollen of Billia show evidence for inter-specific hybridization? (iii) Do the exine morphology and size of pollen in Billia differ from those in Aesculus? Our results from scanning electron microscopy showed that pollen exine morphology is not taxonomically informative in Billia but that there are significant differences in pollen size between red- and white-flowered individuals. Thus, our pollen data support the utility of flower color in Billia for species delimitation. Our assessments of pollen viability do not support hybridization in the genus, but cannot be used to rule it out. Finally, pollen exine morphology may lend some support to an evolutionary origin ofBillia within eastern North American Aesculus. In contrast, data on pollen size suggest that Billia may belong in a topological position outside of Aesculus. 相似文献
56.
57.
This report describes a convenient method for the rapid and efficient
release of N-linked oligosaccharides from low microgram amounts of
glycoproteins. A 96-well MultiScreen assay system containing a
polyvinylidene difluoride (PVDF) membrane is employed to immobilize
glycoproteins for subsequent enzymatic deglycosylation. Recombinant
tissue-type plasminogen activator (rt-PA) is used to demonstrate the
deglycosylation of 0.1-50 micrograms of a glycoprotein. This method enabled
the recovery of a sufficient amount of N-linked oligosaccharides released
enzymatically with peptide N-glycosidase F (PNGaseF) from as little as 0.5
microgram rt-PA for subsequent analysis by matrix-assisted laser
desorption/ionization time-of-flight (MALDI- TOF) mass spectrometry. The
immobilization of rt-PA to the PVDF membrane did not sterically inhibit the
PNGaseF-mediated release of oligosaccharides from rt-PA as determined by
tryptic mapping experiments. Comparison of the oligosaccharides released
from 50 micrograms of rt-PA by either the 96-well plate method or by a
standard solution digestion procedure showed no significant differences in
the profiles obtained by high-pH anion-exchange chromatography with pulsed
amperometric detection (HPAEC-PAD). Both neutral and sialylated
oligosaccharide standards spiked into wells were recovered equally as
determined by HPAEC-PAD. One advantage of this approach is that reduction
and alkylation can be performed on submicrogram amounts of glycoproteins
with easy removal of reagents prior to PNGaseF digestion. In addition, this
method allows 60 glycoprotein samples to be deglycosylated in 1 day with
MALDI-TOF or HPAEC-PAD analysis being performed on the following day.
相似文献
58.
Strasser-Wozak EM Hartmann BL Geley S Sgonc R Böck G AJ Santos Hattmannstorfer R Wolf H Pavelka M Kofler R 《Cell death and differentiation》1998,5(8):687-693
The tumor suppressor p53 has been implicated in gamma irradiation-induced apoptosis. To investigate possible consequences of wild-type p53 loss in leukemia, we studied the effect of a single dose of gamma irradiation upon p53-deficient human T-ALL (acute lymphoblastic leukemia) CCRF - CEM cells. Exposure to 3 - 96 Gy caused p53-independent cell death in a dose and time-dependent fashion. By electron microscopic and other criteria, this cell death was classified as apoptosis. At low to intermediate levels of irradiation, apoptosis was preceded by accumulation of cells in the G2/M phase of the cell division cycle. Expression of Bcl-2 and Bax were not detectably altered after irradiation. Expression of the temperature sensitive mouse p53 V135 mutant induced apoptosis on its own but only slightly increased the sensitivity of CCRF - CEM cells to gamma irradiation. Thus, in these, and perhaps other leukemia cells, a p53- and Bcl-2/Bax-independent mechanism is operative that efficiently senses irradiation effects and translates this signal into arrest in the G2/M phase of the cell cycle and subsequent apoptosis. 相似文献
59.
Transformation-defective v-ski induces MyoD and myogenin expression but not myotube formation. 总被引:7,自引:0,他引:7 下载免费PDF全文
The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation. 相似文献
60.
Fernando Colmenares 《Primates; journal of primatology》1991,32(4):453-463
Interactions of asymmetrical, symmetrical, and unreciprocated greeting, and episodes of aggression and coalitions observed
between 18 males, members of a large multi-harem colony of baboons (Papio hamadryas, P. cynocephalus, and their hybrids) housed in the Madrid Zoo, were recorded throughout 11 time blocks between January 1983 and March 1985.
The major question investigated was whether the demographic characteristics of the colony affected the frequency with which
males used three behavioural strategies, namely, greeting, aggression, and coalitions. It was found that under demographic
conditions eliciting more intense male-male competition, for example, when the ratio of oestrous cycles per adult male was
lower, males exchanged frequent greetings, especially the symmetrical form (i.e. notifying) and solicited more coalitions,
but did not show any significant change in rates of dyadic aggression. Male phenotype-related effects on rates of greeting
and coalitions were found as well. Results are discussed in relation with the suggested role of greeting and of coalition
behaviour as strategies used in the negotiation of changes in competitive social relationship (e.g. consort changeovers).
These strategies are regarded as social tactics alternative to direct aggression. 相似文献