排序方式: 共有70条查询结果,搜索用时 15 毫秒
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Luciano F. Huergo Marcelo C. Assumpo Emanuel M. Souza M. Berenice R. Steffens M. Geoffrey Yates Leda S. Chubatsu Fbio O. Pedrosa 《Applied microbiology》2004,70(10):6320-6323
The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes. 相似文献
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Schwab S Ramos HJ Souza EM Pedrosa FO Yates MG Chubatsu LS Rigo LU 《Archives of microbiology》2007,187(5):379-386
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Bonatto AC Souza EM Oliveira MA Monteiro RA Chubatsu LS Huergo LF Pedrosa FO 《Archives of microbiology》2012,194(8):643-652
PII are signal-transducing proteins that integrate metabolic signals and transmit this information to a large number of proteins. In proteobacteria, PII are modified by GlnD (uridylyltransferase/uridylyl-removing enzyme) in response to the nitrogen status. The uridylylation/deuridylylation cycle of PII is also regulated by carbon and energy signals such as ATP, ADP and 2-oxoglutarate (2-OG). These molecules bind to PII proteins and alter their tridimensional structure/conformation and activity. In this work, we determined the effects of ATP, ADP and 2-OG levels on the in vitro uridylylation of Herbaspirillum seropedicae PII proteins, GlnB and GlnK. Both proteins were uridylylated by GlnD in the presence of ATP or ADP, although the uridylylation levels were higher in the presence of ATP and under high 2-OG levels. Under excess of 2-OG, the GlnB uridylylation level was higher in the presence of ATP than with ADP, while GlnK uridylylation was similar with ATP or ADP. Moreover, in the presence of ADP/ATP molar ratios varying from 10/1 to 1/10, GlnB uridylylation level decreased as ADP concentration increased, whereas GlnK uridylylation remained constant. The results suggest that uridylylation of both GlnB and GlnK responds to 2-OG levels, but only GlnB responds effectively to variation on ADP/ATP ratio. 相似文献
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Marco Antonio Seiki KadowakiJorge Iulek João Alexandre Ribeiro Gonçalves BarbosaFábio de Oliveira Pedrosa Emanuel Maltempi de SouzaLeda Satie Chubatsu Rose Adele MonteiroMarco Aurélio Schüler de Oliveira Maria Berenice Reynaud Steffens 《Biochimica et Biophysica Acta - Proteins and Proteomics》2012,1824(2):359-365
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Thiago E. Rodrigues Edileusa C. M. Gerhardt Marco A. Oliveira Leda S. Chubatsu Fabio O. Pedrosa Emanuel M. Souza Gustavo A. Souza Marcelo Müller‐Santos Luciano F. Huergo 《Molecular microbiology》2014,91(4):751-761
The PII family comprises a group of widely distributed signal transduction proteins. The archetypal function of PII is to regulate nitrogen metabolism in bacteria. As PII can sense a range of metabolic signals, it has been suggested that the number of metabolic pathways regulated by PII may be much greater than described in the literature. In order to provide experimental evidence for this hypothesis a PII protein affinity column was used to identify PII targets in Azospirillum brasilense. One of the PII partners identified was the biotin carboxyl carrier protein (BCCP), a component of the acetyl‐CoA carboxylase which catalyses the committed step in fatty acid biosynthesis. As BCCP had been previously identified as a PII target in Arabidopsis thaliana we hypothesized that the PII–BCCP interaction would be conserved throughout Bacteria. In vitro experiments using purified proteins confirmed that the PII–BCCP interaction is conserved in Escherichia coli. The BCCP–PII interaction required MgATP and was dissociated by increasing 2‐oxoglutarate. The interaction was modestly affected by the post‐translational uridylylation status of PII; however, it was completely dependent on the post‐translational biotinylation of BCCP. 相似文献
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Xiang Wan Can Yang Qiang Yang Hong Xue Nelson LS Tang Weichuan Yu 《BMC bioinformatics》2009,10(1):13-15
Background
The interactions of multiple single nucleotide polymorphisms (SNPs) are highly hypothesized to affect an individual's susceptibility to complex diseases. Although many works have been done to identify and quantify the importance of multi-SNP interactions, few of them could handle the genome wide data due to the combinatorial explosive search space and the difficulty to statistically evaluate the high-order interactions given limited samples. 相似文献30.