全文获取类型
收费全文 | 3195篇 |
免费 | 128篇 |
国内免费 | 14篇 |
出版年
2021年 | 25篇 |
2018年 | 27篇 |
2017年 | 23篇 |
2016年 | 29篇 |
2015年 | 54篇 |
2014年 | 70篇 |
2013年 | 107篇 |
2012年 | 122篇 |
2011年 | 119篇 |
2010年 | 98篇 |
2009年 | 137篇 |
2008年 | 123篇 |
2007年 | 117篇 |
2006年 | 91篇 |
2005年 | 81篇 |
2004年 | 77篇 |
2003年 | 54篇 |
2002年 | 37篇 |
2001年 | 36篇 |
2000年 | 37篇 |
1999年 | 43篇 |
1998年 | 43篇 |
1997年 | 51篇 |
1996年 | 41篇 |
1995年 | 32篇 |
1994年 | 27篇 |
1993年 | 21篇 |
1992年 | 40篇 |
1991年 | 18篇 |
1990年 | 25篇 |
1989年 | 24篇 |
1988年 | 20篇 |
1987年 | 23篇 |
1986年 | 21篇 |
1985年 | 20篇 |
1983年 | 19篇 |
1981年 | 22篇 |
1979年 | 20篇 |
1971年 | 16篇 |
1959年 | 49篇 |
1958年 | 123篇 |
1957年 | 137篇 |
1956年 | 134篇 |
1955年 | 149篇 |
1954年 | 150篇 |
1953年 | 119篇 |
1952年 | 104篇 |
1951年 | 98篇 |
1950年 | 59篇 |
1948年 | 23篇 |
排序方式: 共有3337条查询结果,搜索用时 19 毫秒
11.
12.
MAAS FRANK M.; DE KOK LUIT J.; PETERS JANNY L.; KUIPER PIETER J. C. 《Journal of experimental botany》1987,38(9):1459-1469
Maas, F. M., De Kok, L. J., Peters, J. L. and Kuiper, P. J.C. 1987. A comparative study on the effects of H2S and SO2 fumigationon the growth and accumulation of sulphate and sulphydryl compoundsin Trifolium pratense L., Glycine max Merr. and Phaseolus vulgarisL.J. exp. Bot. 38: 1459-1469. The effects of 025 mm3 dm3 H2S and SO2 on growth andsulphur content of shoots of Trifolium pratense, Glycine maxand Phaseolus vulgaris were studied. After 2 weeks of fumigationthe yield of T. pralense was reduced by 32% by H2S, but notaffected by SO2. Yield of G. max was not affected by H2S, butreduced by 20% by SO2, whereas that of P. vulgaris was increasedby 11% by H2S and not affected by SO2. Increases in sulphydrylcontent were already observed after 24 h of exposure to H2Sand SO2 in all plants. The increase was greatest in T. pratenseand smallest in P. vulgaris and, except for T. pratense, alwaysgreater in the H2S-exposed than the SO2-exposed plants. Oneday of exposure resulted in an increase in sulphate contentonly in the SO2-fumigated plants, with the highest accumulationin T. pratense and the lowest in P. vulgaris. After 2 weeksan increase in sulphate content was also observed in the H2S-exposedplants. This increase was also highest in T. pratense and lowestin P. vulgaris. Transpiration rate was not affected by a 24 h exposure to H2Sor SO2 and was highest in T. pratense, intermediate in G. maxand lowest in P. vulgaris. The order of theoretical rates of deposition of H2S and SO2correlated with the observed increases in sulphydryl contentduring the first 24 h of exposure in both H2S and SO2-fumigatedplants and with the increase in sulphate content in the SO2-exposedplants. The increases in sulphydryl content were only 8% ofthe theoretical H2S and SO2-deposition fluxes, whereas sulphateaccumulation accounted for at least 57% of the theoretical SO2-depositionflux. Key words: Air pollution, clover, French bean, Glutathione, Soybean, sulphur metabolism. 相似文献
13.
This paper reports the experimental transmission of a bird parasite into jirds. Infective larvae of Cardiofilaria nilesi obtained from laboratory colonized Coquillettidia crassipes mosquitoes which had fed on an infected chicken were inoculated subcutaneously into jirds. The number of larvae per jird varied from 10 to 228. Microfilaraemia appeared 22 to 89 days after inoculation of the infective larvae. Experiments were carried out with 24 jirds through six generations extending over a period of 22 months and 17 produced patent infections. At present 8 infected jirds are being maintained in the laboratory; their patent periods ranging from 6 to 13 months. However, the longest patent period observed was about thirteen months. The percentage of adults recovered in autopsied jirds ranged from 0 to 40 with an average of 16. The chicken showed a microfilarial periodicity with the peak microfilarial density around 2200 hours. However, in jirds there was a change in sub-periodicity. This model in the jird may be very useful for the screening of filaricides and in immunological work. 相似文献
14.
A difference in the expression and metabolism of sulfated glycosaminoglycans between rat mammary tumor cells derived from a primary tumor and those from its metastatic lesions has been observed. Cells from the primary tumor possessed about equal quantities of chondroitin sulfate and heparan sulfate on their cell surfaces but released fourfold more chondroitin sulfate than heparan sulfate into their medium. In contrast, cells from distal metastatic lesions expressed approximately 5 times more heparan sulfate than chondroitin sulfate in both medium and cell surface fractions. This was observed to be the result of differential synthesis of the glycosaminoglycans and not of major structural alterations of the individual glycosaminoglycans. The degree of sulfation and size of heparan sulfate were similar for all cells examined. However, chondroitin sulfate, observed to be only chondroitin 4-sulfate, from the metastases-derived cells had a smaller average molecular weight on gel filtration chromatography and showed a decreased quantity of sulfated disaccharides upon degradation with chondroitin ABC lyase compared to the primary tumor derived cells. Major qualitative or quantitative alterations were not observed for hyaluronic acid among the various 13762NF cells. The metabolism of newly synthesized sulfated glycosaminoglycans was also different between cells from primary tumor and metastases. Cells from the primary tumor continued to accumulate glycosaminoglycans in their medium over a 72-h period, while the accumulation of sulfated glycosaminoglycans in the medium of metastases-derived cells showed a plateau after 18-24 h. A pulse-chase kinetics study demonstrated that both heparan sulfate and chondroitin sulfate were degraded by the metastases-derived cells, whereas the primary tumor derived cells degraded only heparan sulfate and degraded it at a slower rate.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
15.
16.
Heat generation in laser irradiated tissue 总被引:1,自引:0,他引:1
A J Welch J A Pearce K R Diller G Yoon W F Cheong 《Journal of biomechanical engineering》1989,111(1):62-68
Many medical applications involving lasers rely upon the generation of heat within the tissue for the desired therapeutic effect. Determination of the absorbed light energy in tissue is difficult in many cases. Although UV wavelengths of the excimer laser and 10.6 microns wavelength of the CO2 laser are absorbed within the first 20 microns of soft tissue, visible and near infrared wavelengths are scattered as well as absorbed. Typically, multiple scattering is a significant factor in the distribution of light in tissue and the resulting heat source term. An improved model is presented for estimating heat generation due to the absorption of a collimated (axisymmetric) laser beam and scattered light at each point r and z in tissue. Heat generated within tissue is a function of the laser power, the shape and size of the incident beam and the optical properties of the tissue at the irradiation wavelength. Key to the calculation of heat source strength is accurate estimation of the light distribution. Methods for experimentally determining the optical parameters of tissue are discussed in the context of the improved model. 相似文献
17.
18.
REINDERS J. E. A.; AS H. VAN; SCHAAFSMA T. J.; DE JAGER P. A.; SHERIFF D. W. 《Journal of experimental botany》1988,39(9):1199-1210
In this paper we demonstrate the study of plant water balanceby the non-invasive measurement of tissue water content andwater flow using proton nuclear magnetic resonance (NMR). Sapvelocity and flux were measured independently in the presenceof an excess of stationary tissue water. The instrumentationdescribed allows automated and unattended measurement of flow-and water content-variables in a well-defined region of theplant over periods of several days, with a time resolution betweensuccessive measurements of c. 5 s. Using this apparatus theeffect of changes in light intensity (day/night rhythm) andrelative humidity on stem tissue water content as well as onthe velocity and flux of xylem sap in the stem were investigatedin a cucumber plant. The results are in agreement with predictionsfrom a simple model for plant water balance, which is basedon water potential, flow rate and resistance to flow. As longas only transpiration is varied, flow rate and water content(or potential) are affected in opposite ways as demonstratedin this paper. In contrast, the model predicts that changesin uptake (resulting from changes in, for example, root resistance)will induce changes in water content and flow in the same direction.An experimental verification of this prediction is given ina subsequent paper, where, in addition, the NMR results arecompared to those obtained with a dendrometer. Key words: Water balance model, Cucumis sativus L., flow, water content, NMR, water balance measurement 相似文献
19.
Stomatal Response of some Cultivated and Wild Tuber-bearing Potatoes in Warm Tropics as Influenced by Water Deficits 总被引:1,自引:0,他引:1
Leaf resistances of 14 cultivated potato genotypes (Solanumspp) and three tuber-bearing wild Solanum species were comparedwhen plants were grown under water stress at two tropical sitesFactors investigated were diurnal changes in leaf resistance,the effect of plant age, transient drought versus well-wateredconditions of potted and field-grown plants These measurementswere carried out in order to determine the stomatal behaviourof tuber-bearing genotypes and species Significant genotypic differences in leaf resistances were notedwithin the cultivated genotypes All genotypes had higher resistanceswhen water-stressed, but LT-7 appeared to have the lowest leafresistances Genetic differences in stomatal behaviour of tuber-bearingSolanum species were confirmed Abaxial stomatal resistancesof water-stressed plants of the species ranged between 1 74and 13 8 s cm1 Stomata of S chacoense were less affectedby drought (three-fold) than S tuberosum (four-fold) The greatesteffect was on S jungasense (five-fold) and on S raphanifoliumThese data show that stomata behaviour among tuber-bearing Solanumspecies is sufficiently different to warrant investigationsof drought-resistance in potato species under dry hot conditions Solanum tuberosum L., Solanum raphanifolium, Solanum chacoense, Solanum jungasense, leaf resistance 相似文献
20.
A New Procedure for the Calculation of Oxygen Diffusion Resistance in Legume Nodules from Flow-through Gas Analysis Data 总被引:1,自引:0,他引:1
MINCHIN F. R.; IANNETTA P. P. M.; FERNANDEZ-PASCUAL M.; DE LORENZO C.; WITTY J. F.; SPRENT J. I. 《Annals of botany》1992,70(3):283-289
Plants of white lupin (Lupinus albus L cv Multolupa) and soyabean(Glycine max L cv Clarke) were grown in controlled-environmentcabinets, subjected to various stresses and their nodular nitrogenaseactivity and total root respiration measured When these measurementswere used to calculate nodular oxygen diffusion resistance,using a simplified equation for Fick's first law of diffusion,it was found that the apparent resistance of stressed nodulesincreased anomalously with decreases in external oxygen concentrationA new analysis procedure is proposed to alleviate this anomalyThis procedure also uses the simplified Fick's law equationbut includes a respiratory contribution to the total oxygenflux across the diffusion barrier which is not coupled to nitrogenaseactivity Also, resistance is modelled as an exponential functionof external oxygen concentration Use of this analysis procedureproduces realistic values for total resistance and providesa characterisation of this resistance into a minimum value andan adjustment factor for changes in external oxygen It is postulatedthat the additional respiration component represents the activityof nodule cortex cells involved in the diffusion barrier, particularlythat of vascular bundles Oxygen diffusion resistance, nodule, nitrogen fixation, respiration 相似文献