首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   78866篇
  免费   6540篇
  国内免费   4883篇
  90289篇
  2024年   138篇
  2023年   901篇
  2022年   2076篇
  2021年   3603篇
  2020年   2328篇
  2019年   2840篇
  2018年   2869篇
  2017年   2029篇
  2016年   2873篇
  2015年   4585篇
  2014年   5295篇
  2013年   5963篇
  2012年   6899篇
  2011年   6358篇
  2010年   3821篇
  2009年   3376篇
  2008年   4116篇
  2007年   3654篇
  2006年   3171篇
  2005年   2680篇
  2004年   2277篇
  2003年   1972篇
  2002年   1730篇
  2001年   1559篇
  2000年   1565篇
  1999年   1447篇
  1998年   847篇
  1997年   797篇
  1996年   808篇
  1995年   736篇
  1994年   688篇
  1993年   530篇
  1992年   818篇
  1991年   657篇
  1990年   601篇
  1989年   531篇
  1988年   421篇
  1987年   362篇
  1986年   336篇
  1985年   299篇
  1984年   221篇
  1983年   199篇
  1982年   112篇
  1981年   118篇
  1980年   86篇
  1979年   147篇
  1978年   84篇
  1977年   95篇
  1975年   111篇
  1974年   116篇
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
131.
The nucleotide sequence of rat liver tRNAAsn   总被引:1,自引:0,他引:1  
The major species of asparagine specific tRNA was isolated from rat liver, degraded to oligonucleotides, and shown to have the nucleotide sequence pG-U-C-U-C-U-G-U-m1G-m2G-C-G-C- A-A-D-C-G-G-D-X-A-G-C-G-C-m2G-ψ-ψ-C-G-G-C-U-Q-U-U-t6A-A-C-C-G- A-A-A-G-m7G-D-U-G-G-U-G-G-Z-ψ-C-G-m1A-G-C-C-C-A-C-C-C-A-G-G-G- A-C-G-C-C-AOH. Although this tRNA contains several modified nucleotides in their expected positions, it is unique in having X, 3-(3-Amino-3-carboxy-n-propyl)uridine in loop I rather than in loop III; Q, 7-(4,5-cis-dihydroxyl-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine in the wobble position of loop II; and Z, an unknown, and presently uncharacterized nucleoside, at position 23 from the 3′ terminus usually occupied by ribothymidine.  相似文献   
132.
Differentiation of channel models by noise analysis.   总被引:1,自引:0,他引:1       下载免费PDF全文
Differentiation of membrane channel models based on fluctuation (or noise) analysis is discussed. The theory is particularly useful in distinguishing a single-conductance model (Hodgkin-Huxley formalism) from a multiconductance model. When applied to the frog node of Ranvier, it seems likely that the potassium channels of the membrane may have multiconductance states.  相似文献   
133.
134.
Abstract— Analysis of whole autopsy brain from a patient with fucosidosis (α-fucosidase deficiency) revealed minor storage of H-antigen glycolipid [Fuc (α, 1→2) Gal-GlcNAc-Gal-Glc-Ceramide] and a slightly abnormal ganglioside composition in the form of a two-fold elevation of GM1 and the presence of a fucose-containing glycolipid (a minor component) which co-migrated with GD1a. The major storage materials in fucosidosis brain were an oligosaccharide (Fuc-Gal-GlcNAc-Man[Fuc-Gal-GlcNAc-Man]-ManGlcNAc) and a disaccharide [Fuc(α, 1→6)-GlcNAc] in the approximate ratio of 5:1. Lesser amounts of a related oligosaccharide (Gal-GlcNAc-Man[Gal-GlcNAc-Man]-Man-GlcNAc) were isolated from the brain of patients with GM1-gangliosidosis (Types I and II) where the major storage material is known to be GM1-ganglioside (Gal (β, 1→3)GalNAc(β, 1→4) [NeuNAcf(α, 2→3) Gal(β, 1→4)Glc-Ceramide). Similarly, a related oligosaccharide (GlcNAc-Man [GlcNAc-Man]-Man-GlcNAc) was isolated from the brain of a patient with a total deficiency of N-acetyl-β-d -hexosaminidase (Sandhoff variant of GM2-gangliosidosis) where the major storage products are known to be GM2-ganglioside (GalNAc (β 1→4) [NeuNAc (α, 2→3)Gal(β, 1→4)Glc-Ceramine) and its asialo derivative. These studies indicate that glycoproteins containing at least 2 mol of l -fucose per oligosaccharide unit are normally catabolized in human brain. Further, it appears that such glycoproteins are initially catabolized by an endo-N-acetylglucosaminidase to release an oligosaccharide which is then degraded by the sequential action of exo-glycosidases.  相似文献   
135.
The general formalism required to treat two-state sliding filament models of muscle contraction, including free energy considerations, is first reviewed and amplified. This formalism is then used to examine, and modify as needed, three models studied previously by Podolsky and Nolan, in which cross-bridge attachment-detachment and ATP turnover are not tightly coupled. No attempt is made here to establish an optimal, self-consistent model of this type because our interest is primarily in methadology rather than in fitting experimental results. But it appears from this preliminary study that such a model, with satisfactory mechanical and thermodynamic properties, could be found. An extremely simple but unrealistic two-state model is also studied which is of interest because it demonstrates the fact that it is possible, in principle at least, for sliding filament models to work with very high thermodynamic efficiencies (50-100 percent). An appendix is included that is concerned with the form of the dependence of certain first-order rate constants on the concentrations of ATP, ADP, and P.  相似文献   
136.
137.
138.
139.
Alternative splicing is critical for development; however, its role in the specification of the three embryonic germ layers is poorly understood. By performing RNA-Seq on human embryonic stem cells (hESCs) and derived definitive endoderm, cardiac mesoderm, and ectoderm cell lineages, we detect distinct alternative splicing programs associated with each lineage. The most prominent splicing program differences are observed between definitive endoderm and cardiac mesoderm. Integrative multi-omics analyses link each program with lineage-enriched RNA binding protein regulators, and further suggest a widespread role for Quaking (QKI) in the specification of cardiac mesoderm. Remarkably, knockout of QKI disrupts the cardiac mesoderm-associated alternative splicing program and formation of myocytes. These changes arise in part through reduced expression of BIN1 splice variants linked to cardiac development. Mechanistically, we find that QKI represses inclusion of exon 7 in BIN1 pre-mRNA via an exonic ACUAA motif, and this is concomitant with intron removal and cleavage from chromatin. Collectively, our results uncover alternative splicing programs associated with the three germ lineages and demonstrate an important role for QKI in the formation of cardiac mesoderm.  相似文献   
140.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号