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排序方式: 共有124条查询结果,搜索用时 15 毫秒
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M. Cristina Cerquetti Rolando Brawer Christian A. Gerdes M. Magdalena Gherardi Daniel O. Sordelli 《FEMS microbiology letters》1995,132(1-2):73-78
Abstract Cellulolytic and xylanolytic polysaccharidase enzyme activities from the multi-enzyme complex of Postia placenta MAD 698 were dissociated. Decayed wood extracts were fractionated by methyl-hydrophobic interaction chromatography and analyzed for reducing sugars and by viscosimetry and Western blot. Fractionated endoglucanases had molecular masses of 35 and 40 kDa on SDS-PAGE. Western blots probed with anti-endoglucanase and anti-xylanase antibodies revealed two endoglucanase bands at 35 and 40 kDa, but no commonality with the major xylanase band at 36 kDa. Anti-endoglucanase antibody inhibited endoglucanase enzyme activity. 相似文献
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Roel MC Jansen Jan W Hofstee Jürgen Wildt Francel WA Verstappen Harro J Bouwmeester Eldert J van Henten 《Plant signaling & behavior》2009,4(9):824-829
A novel approach to support the inspection of greenhouse crops is based on the measurement of volatile organic compounds emitted by unhealthy plants.This approach has attracted some serious interest over the last decade. In pursuit of this interest, we performed several experiments at the laboratory-scale to pinpoint marker volatiles that can be used to indicate certain health problems. In addition to these laboratory experiments, pilot and model studies were performed in order to verify the validity of these marker volatiles under real-world conditions. This paper provides an overview of results and gives an outlook on the use of plant volatiles for plant health monitoring.Key words: plant health, volatiles, plant pathogens, plant infection 相似文献
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Under high levels of radiation (70-100 µW/m2 =175 mV/m), seeds of Brassicaceae Lepidium sativum (cress d’Alinois) never germinated. In fact, the first step of seeds’ germination ‒ e.g. imbibitions of germinal cells ‒ could not occur under radiation, while inside the humid compost such imbibitions occurred and roots slightly developed. When removed from the electromagnetic field, seeds germinated normally. The radiation was, thus, most likely the cause of the non-occurrence of the seeds’ imbibitions and germination. 相似文献
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A new mouse mutation, Sprawling, highlights an essential role for the dynein heavy chain in sensory neuron function, but it lacks the ability of other known heavy-chain mutations to ameliorate neurodegeneration due to defective superoxide dismutase. 相似文献
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Gherardi MM Gómez MI García VE Sordelli DO Cerquetti MC 《FEMS immunology and medical microbiology》2000,29(2):81-88
The protection conferred by temperature-sensitive mutants of Salmonella enteritidis against different wild-type Salmonella serotypes was investigated. Oral immunization with the single temperature-sensitive mutant E/1/3 or with a temperature-sensitive thymine-requiring double mutant (E/1/3T) conferred: (i) significant protection against the homologous wild-type Salmonella strains; (ii) significant cross-protection toward high challenge doses of S. typhimurium. Significant antibody levels against homologous lipopolysaccharide and against homologous and heterologous protein antigens were detected in sera from immunized mice. Moreover, a wide range of protein antigens from different Salmonella O serotypes were recognized by sera from immunized animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized Salmonella antigens from different serotypes. Taken together, these results indicate that temperature-sensitive mutants of S. enteritidis are good candidates for the construction of live vaccines against Salmonella. 相似文献
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