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991.
Christelle Boileau Johanne Martel-Pelletier Judith Caron Philippe Msika Georges B Guillou Caroline Baudouin Jean-Pierre Pelletier 《Arthritis research & therapy》2009,11(2):R41-9
Introduction
The aims of this study were, first, to investigate the in vivo effects of treatment with avocado/soybean unsaponifiables on the development of osteoarthritic structural changes in the anterior cruciate ligament dog model and, second, to explore their mode of action. 相似文献992.
Caroline L. Willis John H. Cummings Graham Neale Glenn R. Gibson 《Current microbiology》1997,35(5):294-298
In contrast to other anaerobic ecosystems, such as marine and estuarine sediments, there is a lack of information on the
nutritional requirements of human gut sulfate-reducing bacteria (SRB). Various substrates stimulated sulfate reduction in
mixed culture, including short-chain fatty acids and other organic acids, alcohols, and amino acids (but not sugars or aromatic
compounds). However, the use of sodium molybdate as a specific inhibitor of sulfate reduction caused an accumulation of ethanol
and malonate only, and reduced the rate of utilization of lactate. This indicates the importance of these electron donors
for sulfate reduction. Since ethanol and lactate are primarily utilized by members of the Desulfovibrio genus, the results suggest a physiologically important role for this group. Experiments with two strains of Desulfovibrio desulfuricans isolated from human feces demonstrated that both were able to reduce sulfite, thiosulfate or nitrate in the absence of sulfate.
In addition, one strain (DsvUC1) was able to grow by fermentative metabolism, although the second strain (DsvFD1) showed more
restricted fermentative growth. The data indicate that desulfovibrios are ecologically the most significant group of SRB in
the human colon, and that colonic isolates belonging to this genus are versatile, in terms of both the electron acceptors
and donors that they are able to utilize.
Received: 24 March 1997 / Accepted: 10 June 1997 相似文献
993.
994.
Mischa R. Müller Kenneth Saunders Christopher Grace Macy Jin Nicole Piche-Nicholas John Steven Ronan O’Dwyer Leeying Wu Lam Khetemenee Yulia Vugmeyster Timothy P. Hickling Lioudmila Tchistiakova Stephane Olland Davinder Gill Allan Jensen Caroline J. Barelle 《MABS-AUSTIN》2012,4(6):673-685
Advances in recombinant antibody technology and protein engineering have provided the opportunity to reduce antibodies to their smallest binding domain components and have concomitantly driven the requirement for devising strategies to increase serum half-life to optimise drug exposure, thereby increasing therapeutic efficacy. In this study, we adopted an immunization route to raise picomolar affinity shark immunoglobulin new antigen receptors (IgNARs) to target human serum albumin (HSA). From our model shark species, Squalus acanthias, a phage display library encompassing the variable binding domain of IgNAR (VNAR) was constructed, screened against target, and positive clones were characterized for affinity and specificity. N-terminal and C-terminal molecular fusions of our lead hit in complex with a naïve VNAR domain were expressed, purified and exhibited the retention of high affinity binding to HSA, but also cross-selectivity to mouse, rat and monkey serum albumin both in vitro and in vivo. Furthermore, the naïve VNAR had enhanced pharmacokinetic (PK) characteristics in both N- and C-terminal orientations and when tested as a three domain construct with naïve VNAR flanking the HSA binding domain at both the N and C termini. Molecules derived from this platform technology also demonstrated the potential for clinical utility by being available via the subcutaneous route of delivery. This study thus demonstrates the first in vivo functional efficacy of a VNAR binding domain with the ability to enhance PK properties and support delivery of multifunctional therapies. 相似文献
995.
Rates of excretion of NH4+ urea and dissolved primary amines(DPA) by Acartia tonsa were measured over two 24-h periods inestuarine mesocosms. Animals ( 相似文献
996.
997.
David A. Sandham Claire Adcock Kamlesh Bala Lucy Barker Zarin Brown Gerald Dubois David Budd Brian Cox Robin A. Fairhurst Markus Furegati Catherine Leblanc Jodie Manini Rachael Profit John Reilly Rowan Stringer Alfred Schmidt Katharine L. Turner Simon J. Watson Jennifer Willis Gareth Williams Caroline Wilson 《Bioorganic & medicinal chemistry letters》2009,19(16):4794-4798
High throughput screening identified a 7-azaindole-3-acetic acid scaffold as a novel CRTh2 receptor antagonist chemotype, which could be optimised to furnish a highly selective compound with good functional potency for inhibition of human eosinophil shape change in whole blood and oral bioavailability in the rat. 相似文献
998.
999.
Moss Lenny; Proakobphol Akraporn; Wiedmann Tien-Wen; Fisher Susan J.; Damsky Caroline H. 《Glycobiology》1994,4(5):567-575
Cytotrophoblasts are the specialized epithelial cells of theplacenta. During the first trimester, a subpopulation of chorionicvillas cytotrophoblasts differentiates along an invasive pathwayand penetrates the maternal endo-metrium, decidua and spiralarterioles. Cytotrophoblast invasiveness declines rapidly duringthe second half of gestation. Isolated cytotrophoblasts of differentgestational ages retain this differential invasiveness in culture.To determine whether the properties of integrin receptors forextracellular matrix molecules differ between invasive and non-invasivecytotrophoblasts, detergent extracts of isolated cytotrophoblastsof different gestational ages, and of first-trimester villousfibroblasts, were immunoprecipitated with subunit-specific anti- 相似文献
1000.