收费全文 | 4046篇 |
免费 | 307篇 |
国内免费 | 2篇 |
2024年 | 4篇 |
2023年 | 41篇 |
2022年 | 65篇 |
2021年 | 162篇 |
2020年 | 106篇 |
2019年 | 102篇 |
2018年 | 175篇 |
2017年 | 125篇 |
2016年 | 213篇 |
2015年 | 276篇 |
2014年 | 271篇 |
2013年 | 373篇 |
2012年 | 398篇 |
2011年 | 326篇 |
2010年 | 211篇 |
2009年 | 171篇 |
2008年 | 223篇 |
2007年 | 214篇 |
2006年 | 178篇 |
2005年 | 140篇 |
2004年 | 126篇 |
2003年 | 100篇 |
2002年 | 92篇 |
2001年 | 31篇 |
2000年 | 24篇 |
1999年 | 20篇 |
1998年 | 21篇 |
1997年 | 17篇 |
1996年 | 7篇 |
1995年 | 15篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 13篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 4篇 |
1987年 | 9篇 |
1985年 | 8篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1971年 | 2篇 |
Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortality. Intestinal microbiota are involved in many processes that benefit a host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism. The aim of this study is to evaluate the in vitro probiotic properties of bacteria isolated from the intestinal content of wild Seriola lalandi. The probiotic potential was evaluated in terms of (i) the antimicrobial activity against vibrios causing outbreaks in farmed fish; (ii) the ability to stimulate genes related to an innate immune response in fish; and (iii) antibiotic resistance. Nineteen isolates identified as Pseudomonas, Shewanella, Psychrobacter, and Acinetobacter showed antimicrobial activity and significant relative expression of cytokines, serum amyloid A protein (SAA), hepcidin, and lysozyme. A positive correlation was observed between the levels of expression and the bacterial load after 24 h of exposure. Pseudomonas isolates showed a level of antibiotic resistance. In conclusion, isolates of the genera Shewanella, Psychrobacter, and Acinetobacter could serve as potential probiotics in S. lalandi culture.
相似文献This study evaluated the in vitro effect of 3.0, 6.0, and 9.0% of green banana pulp (GBP) incorporation in fermented milk on the survival of Lactobacillus paracasei subsp. paracasei LBC 81 subjected to acid stress conditions and in the presence of bile salts. Tolerance to acid stress in pH 2.0 and in the presence of 0.30% of bile salts was evaluated right after the incorporation of the fermented milk in each of these conditions, and also during 3 and 4 h of exposure, respectively. The addition of GBP (3.0%) gives a protective effect on L. paracasei LBC 81 when exposed to stress conditions evaluated, while of 9.0% there is a marked decrease of L. paracasei LBC 81. In the absence of GBP, a decrease of L. paracasei LBC 81 is observed, but lower in the presence of GBP (9.0%).
相似文献