Aptian dinosaur footprints from the Apulian platform (Bisceglie,Southern Italy) in the framework of periadriatic ichnosites

New dinosaur tracks have been found near Bisceglie (Bari, Apulia), on loose blocks ascribed to the Corato Member (late Bedoulian to early Gargasian) of the Calcare di Bari Fm. The material consists of isolated footprints as well as of short trackways of quadrupedal and bipedal dinosaurs. The new tracksite has yielded a quite differentiated dinosaur ichnocoenosis, including theropod, sauropod, thyreophoran and ornithopod footprints.The discovery of early Aptian dinosaur footprints in the limestone of the carbonate platform of southern Italy gives new insights on dinosaur distribution, and new palaeontological constraints for the palaeogeographic reconstruction of the Mediterranean Tethys during the Cretaceous. The analysis of this and others ichnosites of the periadriatic carbonate platforms, gives evidence of repeated emersions and of widespread land-vertebrates dwelling. The characteristics of the associations suggest that the trackmakers did not constitute a real coevolved association but the occasional co-occurrence of taxa after migration.The results emphasize the need of both structural and environmental continuity and walking ways between a southern continent and the periadriatic carbonate platforms during the Early Cretaceous.     

Tourism LCA: state-of-the-art and perspectives

Background, aim and scope  

Records over the last decades indicate a high growth rate for tourism, making it one of the most important industries in the world economy. Since estimates outline a consolidation of this trend, an accurate identification and assessment of the environmental impacts related to the life cycle of tourist products is increasingly necessary. By reviewing and comparing Life Cycle Assessment (LCA) case studies in the tourism sector, this paper aims to identify life cycle approaches that may be used as a basis for the subsequent development of sectorial Life Cycle Thinking guidelines.     

Phosphotyrosine signaling networks in epidermal growth factor receptor overexpressing squamous carcinoma cells

Overexpression and enhanced activation of the epidermal growth factor (EGF) receptor are frequent events in human cancers that correlate with poor prognosis. Anti-phosphotyrosine and anti-EGFr affinity chromatography, isotope-coded muLC-MS/MS, and immunoblot methods were combined to describe and measure signaling networks associated with EGF receptor activation and pharmacological inhibition. The squamous carcinoma cell line HN5, which overexpresses EGF receptor and displays sustained receptor kinase activation, was used as a model system, where pharmacological inhibition of EGF receptor kinase by erlotinib markedly reduced auto and substrate phosphorylation, Src family phosphorylation at EGFR Y845, while increasing total EGF receptor protein. Diverse sets of known and poorly described functional protein classes were unequivocally identified by affinity selection, comprising either proteins tyrosine phosphorylated or complexed therewith, predominantly through EGF receptor and Src family kinases, principally 1) immediate EGF receptor signaling complexes (18%); 2) complexes involved in adhesion and cell-cell contacts (34%); and 3) receptor internalization and degradation signals. Novel and known phosphorylation sites could be located despite the complexity of the peptide mixtures. In addition to interactions with multiple signaling adaptors Grb2, SHC, SCK, and NSP2, EGF receptors in HN5 cells were shown to form direct or indirect physical interactions with additional kinases including ACK1, focal adhesion kinase (FAK), Pyk2, Yes, EphA2, and EphB4. Pharmacological inhibition of EGF receptor kinase activity by erlotinib resulted in reduced phosphorylation of downstream signaling, for example through Cbl/Cbl-B, phospholipase Cgamma (PLCgamma), Erk1/2, PI-3 kinase, and STAT3/5. Focal adhesion proteins, FAK, Pyk2, paxillin, ARF/GIT1, and plakophillin were down-regulated by transient EGF stimulation suggesting a complex balance between growth factor induced kinase and phosphatase activities in the control of cell adhesion complexes. The functional interactions between IGF-1 receptor, lysophosphatidic acid (LPA) signaling, and EGF receptor were observed, both direct and/or indirectly on phospho-Akt, phospho-Erk1/2, and phospho-ribosomal S6.     

Systematic literature review in social life cycle assessment

Purpose

The main purpose of this review is to investigate the methodology of social life cycle assessment (SLCA) through its application to case studies. In addition, the following research aims to define the trends related to the SLCA by researchers and consultants. This study will help to map the current situation and to highlight the hot spots and weaknesses of the application of the SLCA theory.

Methods

The SLCA could be considered as a useful methodology to provide decision support in order to compare products and/or improve the social effects of the life cycle of a product. Furthermore, the results of the case studies analyzed may influence decision makers significantly. For this reason, a systematic literature review of case studies was carried out in which SLCA was applied in order to analyze closely the application of the stages of this methodology. In this study, the major phases of the technical framework for a SLCA were analyzed. Specific attention was paid to detect the positive impacts that emerged in the case studies, which were also studied by administering a questionnaire to the authors of the analyzed case studies and to a number of experts in the field of SLCA.

Results and discussion

The 35 case studies examined in this paper, even though they do not deviate from the 40 identified by the previous processing, are still significantly different in terms of outcome produced. It is important to clarify that the authors who developed the case studies considered the steps defined in the SETAC/SETAC guidelines, borrowed from the ISO 14044 standard.

Conclusions

The data resulting from this analysis could help both practitioners and researchers to understand what the issues are, on which it is still necessary to investigate and work, in order to solidify the SLCA methodology and define its role in the context of life cycle sustainability assessment (LCSA).

     

An Italian tomato “Cuore di Bue” case study: challenges and benefits using subcategory assessment method for social life cycle assessment

Purpose

The main purpose of this study is to present an implementation of the subcategory assessment method (SAM) to the life cycle of an Italian variety of tomato called “Cuore di Bue” produced by an Italian cooperative. The case study was used to use the methodology proposed in compliance with the guidelines of social life cycle assessment (S-LCA) in order to highlight issues for the improvement of SAM. A summary of strengths and weaknesses of the methodology as well as the social performance of the considered Italian tomato is an important result of this case study.

Methods

The methodology used is based on SAM. The UNEP/SETAC guidelines of S-LCA and the complementary methodological sheets were used as main references to carry out SAM, and it was used to assess the social performances of Cuore di Bue. The focus was on the assessment of the following three out of five stakeholder groups presented in the guidelines: workers, local community and consumers. Specific questionnaires have been developed to collect the inventory data related to each stakeholder group and along the product life cycle.

Results and discussion

SAM of Cuore di Bue showed a range of values, between 2 and 3 (C-B) for consumer stakeholder group and mainly 3 (B) for the local community and worker stakeholders. Because the best performance (A) is related to a numerical value of 4, better performances were not identified, owing to no propagation of actions in the value chain. The collective bargaining, transparency, feedback mechanism and privacy are the subcategories with the worst performance, but at the same time with more potential for improvements.

Conclusions

The implementation of SAM on Cuore di Bue allowed us to demonstrate how SAM transforms qualitative data into semi-quantitative information through a score scale that can help a decision maker achieve a product overview. SAM has been implemented on Cuore di Bue; the product assessment, the strengths and weaknesses of the methodology are identified and discussed as well. It has been possible to present the best and worst performances in product life cycle, by identifying the phase or the subcategories with good or bad performance. However, in this case study, as the same company owns most of the product life cycle taken into account, the majority of social performances are identical, and this may represent a limit of the methodology or that more organisations along the life cycle must be taken into account (for example, energy, distribution).

     

Subcategory assessment method for social life cycle assessment. Part 1: methodological framework

Purpose

The aim of this work is to propose an objective method for evaluating subcategories in social life cycle impact assessment (S-LCIA). Methods for assessing subcategories have been available since 2006, but a number of these either fail to include all the subcategories envisaged in the guidelines for S-LCA (UNEP/SETAC 2009) or are subjective in their assessment of each subcategory.

Methods

The methodology is characterized by four steps: (i) the use of the organization as unit process, in which it was decided to assess the social profile of the organization responsible for the processes involved in the product life cycle, (ii) definition of the basic requirement to assess each subcategory, (iii) definition of levels based on the environment context or organizational practice and the data availability and (iv) assignment of a quantitative value.

Results and discussion

The result of the method applied was the development of the subcategory assessment method (SAM). SAM is a characterization model that evaluates subcategories during the impact assessment phase. This method is based on the behaviour of organizations responsible for the processes along the product life cycle, thereby enabling a social performance evaluation. The method, thus, presents levels for each subcategory assessment. Level A indicates that the organization exhibits proactive behaviour by promoting basic requirement (BR) practices along the value chain. Level B means that the organization fulfils the BR. Levels C and D are assigned to organizations that do not meet the BR and are differentiated by their context. The greatest difficulty when developing SAM was the definition of the BR to be used in the evaluation of the subcategories, though many indications were present in the methodological sheets.

Conclusions

SAM makes it possible to go from inventory to subcategory assessment. The method supports evaluation across life cycle products, thereby ensuring a more objective evaluation of the social behaviour of organizations and applicable in different countries.

Recommendations

When using SAM, it is advisable to update the data for the context environment. The method might be improved by using data for the social context that would consider not only the country, but also the region, sector and product concerned. A further improvement could be a subdivision of the levels to better encompass differences between organizations. It is advisable to test SAM by applying it to a case study.