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961.
A Medium for the Isolation of Staphylococci from Foodstuffs 总被引:5,自引:5,他引:0
S ummary . A liquid medium for the isolation of staphylococci from foodstuffs, containing lithium chloride, potassium tellurite and glycine as inhibitors of contaminants other than cocci, is described. Micrococci are inhibited in the cultures by anaerobic conditions produced by agar seals. With this medium staphylococci are easily isolated even when their numbers in foodstuffs are low. 相似文献
962.
Gregorio Oxilia Eugenio Bortolini Giulia Marciani Jessica Cristina Menghi Sartorio Antonino Vazzana Matteo Bettuzzi Daniele Panetta Simona Arrighi Federica Badino Carla Figus Federico Lugli Matteo Romandini Sara Silvestrini Rita Sorrentino Adriana Moroni Carlo Donadio Maria Pia Morigi Viviane Slon Marcello Piperno Sahra Talamo Carmine Collina Stefano Benazzi 《American journal of physical anthropology》2022,179(1):18-30
963.
Pulido Mantas Torcuato Pola Lisa Cerrano Carlo Gambi Maria Cristina Calcinai Barbara 《Hydrobiologia》2022,849(8):1969-1980
Hydrobiologia - Considering the pivotal role played by erosive organisms in the marine habitat and the scanty knowledge of this phenomenon in the Mediterranean Sea, the present study aimed to... 相似文献
964.
Pagotto João Paulo Alves Pessoa Leonardo Antunes Goulart Erivelto Mise Fábio Teruo Ortega Jean Carlo Gonçalves Landgraf Guilherme Okuda 《Hydrobiologia》2022,849(10):2299-2316
Hydrobiologia - Human activities change the environmental conditions of streams and alter their assemblages. However, the environmental factors associated with the change in the ecomorphological... 相似文献
965.
966.
Pavel Mader Rodrigo Mendoza-Sanchez Aman Iqbal Aiping Dong Elena Dobrovetsky Victoria B. Corless Sean K. Liew Scott R. Houliston Renato Ferreira De Freitas David Smil Carlo C. Dela Sena Steven Kennedy Diego B. Diaz Hong Wu Ludmila Dombrovski Abdellah Allali-Hassani Jinrong Min Matthieu Schapira Cheryl H. Arrowsmith 《Bioorganic & medicinal chemistry》2019,27(17):3866-3878
SET domain bifurcated protein 1 (SETDB1) is a human histone-lysine methyltransferase which is amplified in human cancers and was shown to be crucial in the growth of non-small and small cell lung carcinoma. In addition to its catalytic domain, SETDB1 harbors a unique tandem tudor domain which recognizes histone sequences containing both methylated and acetylated lysines, and likely contributes to its localization on chromatin. Using X-ray crystallography and NMR spectroscopy fragment screening approaches, we have identified the first small molecule fragment hits that bind to histone peptide binding groove of the Tandem Tudor Domain (TTD) of SETDB1. Herein, we describe the binding modes of these fragments and analogues and the biophysical characterization of key compounds. These confirmed small molecule fragments will inform the development of potent antagonists of SETDB1 interaction with histones. 相似文献
967.
Lorenzo Lazzaro Elena Barni Giuseppe Brundu Carlo Blasi Consolata Siniscalco 《Plant biosystems》2019,153(5):740-746
In recent decades, multiple actions have been taken to counteract the relentless expansion of invasive alien species as well as to gain a better understanding of their effects on ecosystems. Here, we describe the approach designed by the Italian Botanical Society that is aimed at selecting a list of candidate alien plants to be subjected to a prioritization procedure. We selected a total of 96 species on the basis of data related to their occurrence on both a national and regional scale, their invasiveness and their potential to invade plant communities and/or habitats of community concern. This list represents the first result obtained by applying this standardized workflow and is a first step towards the identification of those alien species that should be included in the national list according to Regulation (EU) n. 1143/2014. 相似文献
968.
To select strains for the bioremediation of Cr(VI)-polluted environments, four highly Cr(VI)-resistant bacterial isolates were identified and characterized using both traditional techniques and a novel approach called phenotype microarrays. The isolates were identified as members of Pseudomonas mendocina (strains 34 and 56) and members of Pseudomonas corrugata (strains 22 and 28). Results showed that it was possible, by varying the carbon/energy source, to decouple bacterial growth and Cr(VI) reduction, inasmuch as some carbon/energy sources were more effective electron donors for chromate reduction, whereas other sources supported growth but not an effective chromate reduction. The isolates were characterized by a novel high-throughput technique, phenotype microarrays (PM)-Biolog, which can test up to 2000 cellular phenotypes simultaneously. The isolates belonging to P. corrugata had PM profiles different from those of the isolates belonging to P. mendocina. Such differences were related to the capacity of the isolates to resist various chemicals, pH values, and osmolytic substances. With the PM technique a very large amount of information about the fitness of isolates in the presence of different stressors could be obtained. 相似文献
969.
Marco Testa Bianca Rocca Lucia Spath Franco O Ranelletti Giovanna Petrucci Giovanni Ciabattoni Fabio Naro Stefano Schiaffino Massimo Volpe Carlo Reggiani 《Journal of applied physiology》2007,103(4):1412-1418
Conflicting data have been reported on cyclooxygenase (COX)-1 and COX-2 expression and activity in striated muscles, including skeletal muscles and myocardium, in particular it is still unclear whether muscle cells are able to produce prostaglandins (PGs). We characterized the expression and enzymatic activity of COX-1 and COX-2 in the skeletal muscles and in the myocardium of mice, rats and humans. By RT-PCR, COX-1 and COX-2 mRNAs were observed in homogenates of mouse and rat hearts, and in different types of skeletal muscles from all different species. By Western blotting, COX-1 and -2 proteins were detected in skeletal muscles and hearts from rodents, as well as in skeletal muscles from humans. Immunoperoxidase stains showed that COX-1 and -2 were diffusely expressed in the myocytes of different muscles and in the myocardiocytes from all different species. In the presence of arachidonic acid, which is the COX enzymatic substrate, isolated skeletal muscle and heart samples from rodents released predominantly PGE(2). The biosynthesis of PGE(2) was reduced between 50 and 80% (P < 0.05 vs. vehicle) in the presence of either COX-1- or COX-2-selective blockers, demonstrating that both isoforms are enzymatically active. Exogenous PGE(2) added to isolated skeletal muscle preparations from rodents did not affect contraction, whereas it significantly fastened relaxation of a slow type muscle, such as soleus. In conclusion, COX-1 and COX-2 are expressed and enzymatically active in myocytes of skeletal muscles and hearts of rodents and humans. PGE(2) appears to be the main product of COX activity in striated muscles. 相似文献
970.
Vascotto C Salzano AM D'Ambrosio C Fruscalzo A Marchesoni D di Loreto C Scaloni A Tell G Quadrifoglio F 《Journal of proteome research》2007,6(1):160-170
Preeclampsia is a pregnancy-specific hypertensive syndrome and a major cause of maternal and fetal morbidity and mortality. At the present time, no reliable screening tests to identify women at risk are available. We have compared the amniotic fluids (AF) proteomic maps of five preeclamptic patients with those of five controls. The analysis was carried out by two-dimensional electrophoresis followed by peptide mapping and tandem mass spectrometric analysis. Besides the implementation of the previously published AF proteomic maps, our results show that transthyretin (TTR), the protein responsible for transporting both the thyroid hormone tyroxine and the retinol binding protein, is present in the AF of both preeclamptic and control women as a mixture of dimeric and post-translationally modified monomeric forms. Although the nature of these forms is similar in both groups, the preeclamptic women showed a significant increase in the amount of monomeric proteins with respect to the control group. Since the TTR monomeric forms are the results of different oxidizing reactions, we hypothesize that the higher oxidative stress in preeclampsia is the major destabilizing factor of the TTR functional dimeric form in the preeclamptic women. 相似文献