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91.
Arik Kershenbaum Daniel T. Blumstein Marie A. Roch Çağlar Akçay Gregory Backus Mark A. Bee Kirsten Bohn Yan Cao Gerald Carter Cristiane Cäsar Michael Coen Stacy L. DeRuiter Laurance Doyle Shimon Edelman Ramon Ferrer‐i‐Cancho Todd M. Freeberg Ellen C. Garland Morgan Gustison Heidi E. Harley Chloé Huetz Melissa Hughes Julia Hyland Bruno Amiyaal Ilany Dezhe Z. Jin Michael Johnson Chenghui Ju Jeremy Karnowski Bernard Lohr Marta B. Manser Brenda McCowan Eduardo Mercado III Peter M. Narins Alex Piel Megan Rice Roberta Salmi Kazutoshi Sasahara Laela Sayigh Yu Shiu Charles Taylor Edgar E. Vallejo Sara Waller Veronica Zamora‐Gutierrez 《Biological reviews of the Cambridge Philosophical Society》2016,91(1):13-52
Animal acoustic communication often takes the form of complex sequences, made up of multiple distinct acoustic units. Apart from the well‐known example of birdsong, other animals such as insects, amphibians, and mammals (including bats, rodents, primates, and cetaceans) also generate complex acoustic sequences. Occasionally, such as with birdsong, the adaptive role of these sequences seems clear (e.g. mate attraction and territorial defence). More often however, researchers have only begun to characterise – let alone understand – the significance and meaning of acoustic sequences. Hypotheses abound, but there is little agreement as to how sequences should be defined and analysed. Our review aims to outline suitable methods for testing these hypotheses, and to describe the major limitations to our current and near‐future knowledge on questions of acoustic sequences. This review and prospectus is the result of a collaborative effort between 43 scientists from the fields of animal behaviour, ecology and evolution, signal processing, machine learning, quantitative linguistics, and information theory, who gathered for a 2013 workshop entitled, ‘Analysing vocal sequences in animals’. Our goal is to present not just a review of the state of the art, but to propose a methodological framework that summarises what we suggest are the best practices for research in this field, across taxa and across disciplines. We also provide a tutorial‐style introduction to some of the most promising algorithmic approaches for analysing sequences. We divide our review into three sections: identifying the distinct units of an acoustic sequence, describing the different ways that information can be contained within a sequence, and analysing the structure of that sequence. Each of these sections is further subdivided to address the key questions and approaches in that area. We propose a uniform, systematic, and comprehensive approach to studying sequences, with the goal of clarifying research terms used in different fields, and facilitating collaboration and comparative studies. Allowing greater interdisciplinary collaboration will facilitate the investigation of many important questions in the evolution of communication and sociality. 相似文献
92.
Michael F. Curran Samuel E. Cox Timothy J. Robinson Blair L. Robertson Karen J. Rogers Zoe A. Sherman Todd A. Adams Calvin F. Strom Peter D. Stahl 《Restoration Ecology》2019,27(5):974-980
Land reclamation associated with natural gas development has become increasingly important to mitigate land surface disturbance in western North America. Since well pads occur on sites with multiple land use and ownership, the progress and outcomes of these efforts are of interest to multiple stakeholders including industry, practitioners and consultants, regulatory agents, private landowners, and the scientific community. Reclamation success criteria often vary within, and among, government agencies and across land ownership type. Typically, reclamation success of a well pad is judged by comparing vegetation cover from a single transect on the pad to a single transect in an adjacent reference site and data are collected by a large number of technicians with various field monitoring skills. We utilized “SamplePoint” image analysis software and a spatially balanced sampling design, called balanced acceptance sampling, to demonstrate how spatially explicit quantitative data can be used to determine if sites are meeting various reclamation success criteria and used chi‐square tests to show how sites in vegetation percent cover differ from a statistical standpoint. This method collects field data faster than traditional methods. We demonstrate how quantitative and spatially explicit data can be utilized by multiple stakeholders, how it can improve upon current reference site selection, how it can satisfy reclamation monitoring requirements for multiple regulatory agencies, how it may help improve future seed mix selection, and discuss how it may reduce costs for operations responsible for reclamation and how it may reduce observer bias. 相似文献
93.
The cosmopolitan nonindigenous sea anemone, Diadumene lineata, has been recorded from the Pacific coast of Canada since 1859. Despite its long historical presence in the region, the factors influencing the distribution of this species in British Columbia, Canada, are poorly understood, as are the factors that may restrict its local abundance. We conducted large‐scale surveys of D. lineata during the winter and summer of 2016, coupled with higher frequency surveys at one focal site. Furthermore, we used laboratory and field experiments to determine the potential importance of a large‐scale driver, salinity, and a small‐scale driver, microhabitat within boulders, in setting distributional limits at various spatial scales. Our results confirm that D. lineata is widely distributed within the Salish Sea. This broad distribution may be facilitated by post‐introduction dispersal; we observed anemones attached to floating substrata, and a few mature specimens contained eggs, suggesting that reproduction is perhaps not limited to asexual fission. We found that D. lineata was abundant throughout the year, although absent from the lowest salinity sites. This corresponded with our laboratory experiments, in which survival of individuals of D. lineata declined at salinities below 10 psu, and no survivors were observed at or below 2 psu. Manipulative experiments in the field demonstrated that survival was high under rocks where D. lineata is commonly found, but was reduced on the tops of rocks, highlighting the importance of microhabitat refugia in buffering temperature and reducing desiccation. A better understanding of the factors that limit the broad‐scale and local distribution of this anemone are an important step in determining potential ecological impacts of this organism and how distributions may shift into the future. 相似文献
94.
Endoglin is required for myogenic differentiation potential of neural crest stem cells 总被引:1,自引:0,他引:1
Mancini ML Verdi JM Conley BA Nicola T Spicer DB Oxburgh LH Vary CP 《Developmental biology》2007,308(2):520-533
Genetic studies show that TGFbeta signaling is essential for vascular development, although the mechanism through which this pathway operates is incompletely understood. Here we demonstrate that the TGFbeta auxiliary coreceptor endoglin (eng, CD105) is expressed in a subset of neural crest stem cells (NCSCs) in vivo and is required for their myogenic differentiation. Overexpression of endoglin in the neural crest caused pericardial hemorrhaging, correlating with altered vascular smooth muscle cell investment in the walls of major vessels and upregulation of smooth muscle alpha-actin protein levels. Clonogenic differentiation assay of NCSCs derived from neural tube explants demonstrated that only NCSC expressing high levels of endoglin (NCSC(CD105+)) had myogenic differentiation potential. Furthermore, myogenic potential was deficient in NCSCs obtained from endoglin null embryos. Expression of endoglin in NCSCs declined with age, coinciding with a reduction in both smooth muscle differentiation potential and TGFbeta1 responsiveness. These findings demonstrate a cell autonomous role for endoglin in smooth muscle cell specification contributing to vascular integrity. 相似文献
95.
The detailed composition and structure of the Caenorhabditis elegans surface are unknown. Previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. Infection by Microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect surface characteristics. We report that biofilms produced by Yersinia pestis and Y. pseudotuberculosis, which bind the C. elegans surface predominantly on the head, can be used to identify additional surface-determining genes. A screen for C. elegans mutants with a biofilm absent on the head (Bah) phenotype identified three novel genes: bah-1, bah-2, and bah-3. The bah-1 and bah-2 mutants have slightly fragile cuticles but are neither Srf nor Bus, suggesting that they are specific for surface components involved in biofilm attachment. A bah-3 mutant has normal cuticle integrity, but shows a stage-specific Srf phenotype. The screen produced alleles of five known surface genes: srf-2, srf-3, bus-4, bus-12, and bus-17. For the X-linked bus-17, a paternal effect was observed in biofilm assays. 相似文献
96.
Leigh‐Ann Woolley Hayley M. Geyle Brett P. Murphy Sarah M. Legge Russell Palmer Christopher R. Dickman John Augusteyn Sarah Comer Tim S. Doherty Charlie Eager Glenn Edwards Dan K.P. Harley Ian Leiper Peter J. McDonald Hugh W. McGregor Katherine E. Moseby Cecilia Myers John L. Read Joanna Riley Danielle Stokeld Jeff M. Turpin John C.Z. Woinarski 《Mammal Review》2019,49(4):354-368
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97.
The sex-determining factor SRY is a DNA-binding protein that diverts primordial gonads from the ovarian pathway toward male differentiation to form testes. It gains access to the nucleus through two distinct nuclear localization signals (NLSs) that flank the high mobility group (HMG) DNA-binding domain, but the mechanisms through which these NLSs operate have not been studied. In this study, we reconstitute the nuclear import of SRY in vitro, demonstrating a lack of requirement for exogenous factors for nuclear accumulation and a significant reduction in nuclear transport in the presence of antibodies to importin beta but not importin alpha. Using a range of quantitative binding assays including enzyme-linked immunosorbent assay, fluorescence polarization, and native gel mobility electrophoresis, we assess the binding of importins to SRY, demonstrating a high affinity recognition (in the low nm range) by Imp beta independent of Imp alpha. In assessing the contribution of each NLS, we found that the N-terminal NLS was recognized poorly by importins, whereas the C-terminal NLS was bound by importin beta with similar affinity to SRY. We also found that RanGTP, but not RanGDP, could dissociate the SRY-importin beta complex in solution using FP. We describe a novel double-fluorescent label DNA binding assay to demonstrate mutual exclusivity between importin beta recognition and DNA binding on the part of SRY, which may represent an alternative release mechanism upon nuclear entry. This study represents the first characterization of the nuclear import pathway for a HMG domain-containing protein. Importantly, it demonstrates for the first time that recognition of SRY by Imp beta is of comparable affinity to that with which Imp alpha/beta recognizes conventional NLS-containing substrates. 相似文献
98.
Evidence for a susceptibility gene, SLEV1, on chromosome 17p13 in families with vitiligo-related systemic lupus erythematosus 总被引:9,自引:0,他引:9 下载免费PDF全文
Nath SK Kelly JA Namjou B Lam T Bruner GR Scofield RH Aston CE Harley JB 《American journal of human genetics》2001,69(6):1401-1406
Both systemic lupus erythematosus (SLE) and vitiligo are autoimmune disorders that have strong evidence of complex genetic contributions to their etiology, but, to date, efforts using genetic linkage to find the susceptibility genes for either phenotype have met with limited success. Since autoimmune diseases are thought to share at least some of their genetic origins, and since only a small minority (16 of 92) of the European-American pedigrees multiplex for SLE in our collection have one or more affected members with vitiligo, we hypothesized that these pedigrees might be more genetically homogeneous at loci important to both SLE and vitiligo and, hence, have increased power for detection of linkage. We therefore evaluated genomewide microsatellite-marker-scan data for markers at an average marker density of approximately 11 cM in these 16 European-American pedigrees and identified a significant linkage at 17p13, where the maximum multipoint parametric LOD score was 3.64 (P<4.3x10(-5)) and the nonparametric linkage score was 4.02 (P<2.8x10(-5)), respectively. The segregation behavior of this linkage suggests a recessive mode of inheritance with a virtually homogeneous genetic effect in these 16 pedigrees. These results support the hypotheses that SLE and vitiligo may share important genetic effects and that sampling on the basis of clinical covariates dramatically improves power to identify genetic effects. 相似文献
99.
100.
The cytoplasmic compartments occupied by exocytosing herpes simplex virus (HSV) are poorly defined. It is unclear which organelles contain the majority of trafficking virions and which are occupied by virions on a productive rather than defective assembly pathway. These problems are compounded by the fact that HSV-infected cells produce virus continuously over many hours. All stages in viral assembly and export therefore coexist, making it impossible to determine the sequence of events and their kinetics. To address these problems, we have established assays to monitor the presence of capsids and enveloped virions in cell extracts and prepared HSV-containing organelles from normally infected cells and from cells undergoing a single synchronized wave of viral egress. We find that, in both cases, HSV particles exit the nucleus and accumulate in organelles which cofractionate with the trans-Golgi network (TGN) and endosomes. In addition to carrying enveloped infectious virions in their lumen, HSV-bearing organelles also displayed nonenveloped capsids attached to their cytoplasmic surface. Neutralization of organellar pH by chloroquine or bafilomycin A resulted in the accumulation of noninfectious enveloped particles. We conclude that the organelles of the TGN/endocytic network play a key role in the assembly and trafficking of infectious HSV. 相似文献