A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products

A desaturase with 83% sequence identity to the coriander delta(4)-16:0-ACP desaturase was isolated from developing seeds of Hedera helix (English ivy). Expression of the ivy desaturase in Arabidopsis resulted in the accumulation of 16:1delta(4) and its expected elongation product 18:1delta(6) (petroselinic acid). Expression in Escherichia coli resulted in the accumulation of soluble, active protein that was purified to apparent homogeneity. In vitro assays confirmed delta(4) desaturation with 16:0-ACP; however, with 18:0-acyl acyl carrier protein (ACP) desaturation occurred at the delta(9) position. The ivy desaturase also converted 16:1delta(9)-ACP and 18:1delta(9)-ACP to the corresponding delta(4,9) dienes. These data suggest at least two distinct substrate binding modes, one placing C4 at the diiron active site and the other placing C9 at the active site. In the latter case, 18:0 would likely bind in an extended conformation as described for the castor desaturase with 9-carbons accommodated in the cavity beyond the dirron site. However, delta(4) desaturation would require the accommodation of 12 carbons for C16 substrates or 14 carbons for C18 substrates. The amino acids lining the substrate binding cavity of ivy and castor desaturases are conserved except for T117R and P179I (castor/ivy). Paradoxically, both substitutions, when introduced into the castor desaturase, favored the binding of shorter acyl chains. Thus, it seems likely that delta(4) desaturation would require a non-extended, perhaps U-shaped, substrate conformation. A cis double bond may facilitate the initiation of such a non-extended conformation in the monounsaturated substrates. The multifunctional properties of the ivy desaturase make it well suited for further dissection of the determinants of regiospecificity.     

Quantification of the magnification and distortion effects of a pediatric flexible video-bronchoscope

Background

Flexible video bronchoscopes, in particular the Olympus BF Type 3C160, are commonly used in pediatric respiratory medicine. There is no data on the magnification and distortion effects of these bronchoscopes yet important clinical decisions are made from the images. The aim of this study was to systematically describe the magnification and distortion of flexible bronchoscope images taken at various distances from the object.

Methods

Using images of known objects and processing these by digital video and computer programs both magnification and distortion scales were derived.

Results

Magnification changes as a linear function between 100 mm (×1) and 10 mm (×9.55) and then as an exponential function between 10 mm and 3 mm (×40) from the object. Magnification depends on the axis of orientation of the object to the optic axis or geometrical axis of the bronchoscope. Magnification also varies across the field of view with the central magnification being 39% greater than at the periphery of the field of view at 15 mm from the object. However, in the paediatric situation the diameter of the orifices is usually less than 10 mm and thus this limits the exposure to these peripheral limits of magnification reduction. Intraclass correlations for measurements and repeatability studies between instruments are very high, r = 0.96. Distortion occurs as both barrel and geometric types but both types are heterogeneous across the field of view. Distortion of geometric type ranges up to 30% at 3 mm from the object but may be as low as 5% depending on the position of the object in relation to the optic axis.

Conclusion

We conclude that the optimal working distance range is between 40 and 10 mm from the object. However the clinician should be cognisant of both variations in magnification and distortion in clinical judgements.     

Formation of conjugated delta8,delta10-double bonds by delta12-oleic-acid desaturase-related enzymes: biosynthetic origin of calendic acid

Divergent forms of the plant Delta(12)-oleic-acid desaturase (FAD2) have previously been shown to catalyze the formation of acetylenic bonds, epoxy groups, and conjugated Delta(11),Delta(13)-double bonds by modification of an existing Delta(12)-double bond in C(18) fatty acids. Here, we report a class of FAD2-related enzymes that modifies a Delta(9)-double bond to produce the conjugated trans-Delta(8),trans-Delta(10)-double bonds found in calendic acid (18:3Delta(8trans,10trans,12cis)), the major component of the seed oil of Calendula officinalis. Using an expressed sequence tag approach, cDNAs for two closely related FAD2-like enzymes, designated CoFADX-1 and CoFADX-2, were identified from a C. officinalis developing seed cDNA library. The deduced amino acid sequences of these polypeptides share 40-50% identity with those of other FAD2 and FAD2-related enzymes. Expression of either CoFADX-1 or CoFADX-2 in somatic soybean embryos resulted in the production of calendic acid. In embryos expressing CoFADX-2, calendic acid accumulated to as high as 22% (w/w) of the total fatty acids. In addition, expression of CoFADX-1 and CoFADX-2 in Saccharomyces cerevisiae was accompanied by calendic acid accumulation when induced cells were supplied exogenous linoleic acid (18:2Delta(9cis,12cis)). These results are thus consistent with a route of calendic acid synthesis involving modification of the Delta(9)-double bond of linoleic acid. Regiospecificity for Delta(9)-double bonds is unprecedented among FAD2-related enzymes and further expands the functional diversity found in this family of enzymes.     

Production of high levels of poly‐3‐hydroxybutyrate in plastids of Camelina sativa seeds

Poly‐3‐hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed‐specific promoters for expression of transgenes. Genes encoding PHB biosynthetic enzymes were modified at the N‐terminus to encode a plastid targeting signal. PHB levels of up to 15% of the mature seed weight were measured in single sacrificed T₁ seeds with a genetic construct containing the oleosin and glycinin promoters. A more detailed analysis of the PHB production potential of two of the best performing binary vectors in a Camelina line bred for larger seed size yielded lines containing up to 15% polymer in mature T₂ seeds. Transmission electron microscopy showed the presence of distinct granules of PHB in the seeds. PHB production had varying effects on germination, emergence and survival of seedlings. Once true leaves formed, plants grew normally and were able to set seeds. PHB synthesis lowered the total oil but not the protein content of engineered seeds. A change in the oil fatty acid profile was also observed. High molecular weight polymer was produced with weight‐averaged molecular weights varying between 600 000 and 1 500 000, depending on the line. Select lines were advanced to later generations yielding a line with 13.7% PHB in T₄ seeds. The levels of polymer produced in this study are the highest reported to date in a seed and are an important step forward for commercializing an oilseed‐based platform for PHB production.     

The Role of Surface and Subsurface Processes in Keeping Pace with Sea Level Rise in Intertidal Wetlands of Moreton Bay,Queensland, Australia

Increases in the elevation of the soil surfaces of mangroves and salt marshes are key to the maintenance of these habitats with accelerating sea level rise. Understanding the processes that give rise to increases in soil surface elevation provides science for management of landscapes for sustainable coastal wetlands. Here, we tested whether the soil surface elevation of mangroves and salt marshes in Moreton Bay is keeping up with local rates of sea level rise (2.358 mm y⁻¹) and whether accretion on the soil surface was the most important process for keeping up with sea level rise. We found variability in surface elevation gains, with sandy areas in the eastern bay having the highest surface elevation gains in both mangrove and salt marsh (5.9 and 1.9 mm y⁻¹) whereas in the muddier western bay rates of surface elevation gain were lower (1.4 and −0.3 mm y⁻¹ in mangrove and salt marsh, respectively). Both sides of the bay had similar rates of surface accretion (~7–9 mm y⁻¹ in the mangrove and 1–3 mm y⁻¹ in the salt marsh), but mangrove soils in the western bay were subsiding at a rate of approximately 8 mm y⁻¹, possibly due to compaction of organic sediments. Over the study surface elevation increments were sensitive to position in the intertidal zone (higher when lower in the intertidal) and also to variation in mean sea level (higher at high sea level). Although surface accretion was the most important process for keeping up with sea level rise in the eastern bay, subsidence largely negated gains made through surface accretion in the western bay indicating a high vulnerability to sea level rise in these forests.     

Single cell transcriptomics of neighboring hyphae of <Emphasis Type="Italic">Aspergillus niger</Emphasis>

Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.     

Living with myotonic dystrophy; what can be learned from couples? a qualitative study

Background  

Myotonic dystrophy type 1 (MD1) is one of the most prevalent neuromuscular diseases, yet very little is known about how MD1 affects the lives of couples and how they themselves manage individually and together. To better match health care to their problems, concerns and needs, it is important to understand their perspective of living with this hereditary, systemic disease.     

Arabidopsis 3-Ketoacyl-Coenzyme A Synthase9 Is Involved in the Synthesis of Tetracosanoic Acids as Precursors of Cuticular Waxes,Suberins, Sphingolipids,and Phospholipids

Very-long-chain fatty acids (VLCFAs) with chain lengths from 20 to 34 carbons are involved in diverse biological functions such as membrane constituents, a surface barrier, and seed storage compounds. The first step in VLCFA biosynthesis is the condensation of two carbons to an acyl-coenzyme A, which is catalyzed by 3-ketoacyl-coenzyme A synthase (KCS). In this study, amino acid sequence homology and the messenger RNA expression patterns of 21 Arabidopsis (Arabidopsis thaliana) KCSs were compared. The in planta role of the KCS9 gene, showing higher expression in stem epidermal peels than in stems, was further investigated. The KCS9 gene was ubiquitously expressed in various organs and tissues, including roots, leaves, and stems, including epidermis, silique walls, sepals, the upper portion of the styles, and seed coats, but not in developing embryos. The fluorescent signals of the KCS9::enhanced yellow fluorescent protein construct were merged with those of BrFAD2::monomeric red fluorescent protein, which is an endoplasmic reticulum marker in tobacco (Nicotiana benthamiana) epidermal cells. The kcs9 knockout mutants exhibited a significant reduction in C24 VLCFAs but an accumulation of C20 and C22 VLCFAs in the analysis of membrane and surface lipids. The mutant phenotypes were rescued by the expression of KCS9 under the control of the cauliflower mosaic virus 35S promoter. Taken together, these data demonstrate that KCS9 is involved in the elongation of C22 to C24 fatty acids, which are essential precursors for the biosynthesis of cuticular waxes, aliphatic suberins, and membrane lipids, including sphingolipids and phospholipids. Finally, possible roles of unidentified KCSs are discussed by combining genetic study results and gene expression data from multiple Arabidopsis KCSs.Very-long-chain fatty acids (VLCFAs) are fatty acids of 20 or more carbons in length and are essential precursors of functionally diverse lipids, cuticular waxes, aliphatic suberins, phospholipids, sphingolipids, and seed oils in the Brassicaceae. These lipids are involved in various functions, such as acting as protective barriers between plants and the environment, impermeable barriers to water and ions, energy-storage compounds in seeds, structural components of membranes, and lipid signaling, which is involved in the hypersensitive response (Pollard et al., 2008; Kunst and Samuels, 2009; Franke et al., 2012). VLCFAs are synthesized by the microsomal fatty acid elongase complex, which catalyzes the cyclic addition of a C2 moiety obtained from malonyl-CoA to C16 or C18 acyl-CoA. The fatty acid elongation process has been shown to proceed through a series of four reactions: condensation of the C2 carbon moiety to acyl-CoA by 3-ketoacyl coenzyme A synthase (KCS), reduction of KCS by 3-ketoacyl coenzyme A reductase (KCR), dehydration of 3-hydroxyacyl-CoA by 3-hydroxyacyl-CoA dehydratase (PAS2), and reduction of trans-2,3-enoyl-CoA by trans-2-enoyl-CoA reductase (ECR). Except for KCS isoforms with redundancy, disruption of KCR1, ECR/ECERIFERUM10 (CER10), or PAS2 exhibited severe morphological abnormalities and embryo lethality, suggesting that VLCFA homeostasis is essential for plant developmental processes (Zheng et al., 2005; Bach et al., 2008; Beaudoin et al., 2009).Cuticular waxes that cover plant aerial surfaces are known to be involved in limiting nonstomatal water loss and gaseous exchanges (Boyer et al., 1997; Riederer and Schreiber, 2001), repelling lipophilic pathogenic spores and dust (Barthlott and Neinhuis, 1997), and protecting plants from UV light (Reicosky and Hanover, 1978). VLCFAs that are synthesized in the epidermal cells are either directly used or further modified into aldehydes, alkanes, secondary alcohols, ketones, primary alcohols, and wax esters for the synthesis of cuticular waxes. Reverse genetic analysis and Arabidopsis (Arabidopsis thaliana) epidermal peel microarray analysis (Suh et al., 2005) has enabled the research community to identify the functions of many genes involved in cuticular wax biosynthesis (Kunst and Samuels, 2009): CER1 (Bourdenx et al., 2011; Bernard et al., 2012), WAX2/CER3 (Chen et al., 2003; Rowland et al., 2007; Bernard et al., 2012), and MAH1(Greer et al., 2007; Wen and Jetter, 2009) have been shown to be involved in the decarbonylation pathway to form aldehydes, alkanes, secondary alcohols, and ketones, and acyl-coenzyme A reductase (FAR; Aarts et al., 1997; Rowland et al., 2006) and WSD1 (Li et al., 2008) have been shown to be involved in the decarboxylation pathway for the synthesis of primary alcohols and wax esters. The export of wax precursors to the extracellular space is mediated by a heterodimer of the ATP-binding cassette transporters in the plasma membrane (Pighin et al., 2004; Bird et al., 2007; McFarlane et al., 2010). In addition, glycosylphosphatidylinositol-anchored LTP (LTPG1) and LTPG2 contribute either directly or indirectly to the export of cuticular wax (DeBono et al., 2009; Lee et al., 2009; Kim et al., 2012).VLCFAs that are synthesized in the endodermis of primary roots, seed coats, and the chalaza-micropyle region of seeds are used as precursors for the synthesis of aliphatic suberins. The suberin layer is known to function as a barrier against uncontrolled water, gas, and ion loss and provides protection from environmental stresses and pathogens (Pollard et al., 2008; Franke et al., 2012). For aliphatic suberin biosynthesis, the ω-carbon of the VLCFAs is oxidized by the fatty acyl ω-hydroxylase (Xiao et al., 2004; Li et al., 2007; Höfer et al., 2008; Molina et al., 2008, 2009; Compagnon et al., 2009; Li-Beisson et al., 2009), and the ω-hydroxy VLCFAs are further oxidized into α,ω-dicarboxylic acids by the HOTHEAD-like oxidoreductase (Kurdyukov et al., 2006). α,ω-Dicarboxylic acids are acylated to glycerol-3-P via acyl-CoA:glycerol-3-P acyltransferase (Beisson et al., 2007; Li et al., 2007; Li-Beisson et al., 2009; Yang et al., 2010) or to ferulic acid. In addition, C18, C20, and C22 fatty acids are also reduced by FAR enzymes to primary fatty alcohols, which are a common component in root suberin (Vioque and Kolattukudy, 1997). Finally, the aliphatic suberin precursors are likely to be extensively polymerized and cross linked with the polysaccharides or lignins in the cell wall.In addition, VLCFAs are found in sphingolipids, including glycosyl inositolphosphoceramides, glycosylceramides, and ceramides and phospholipids, such as phosphatidylethanolamine (PE) and phosphatidyl-Ser (PS), which are present in the extraplastidial membrane (Pata et al., 2010; Yamaoka et al., 2011). For sphingolipid biosynthesis, VLCFA-CoAs and Ser are condensed to form 3-keto-sphinganine, which is subsequently reduced to produce sphinganine, a long chain base (LCB). LCBs are known to be further modified by 4-hydroxylation, 4-desaturation, and 8-desaturation (Lynch and Dunn, 2004; Chen et al., 2006, 2012; Pata et al., 2010). The additional VLCFAs are linked with 4-hydroxy LCBs via an amino group to form ceramides (Chen et al., 2008). The presence of VLCFA in sphingolipids may contribute to an increase of their hydrophobicity, membrane leaflet interdigitation, and the transition from a fluid to a gel phase, which is required for microdomain formation. In plants, PS is synthesized from CDP-diacylglycerol and Ser by PS synthase or through an exchange reaction between a phospholipid head group and Ser by a calcium-dependent base-exchange-type PS synthase (Vincent et al., 1999; Yamaoka et al., 2011). PE biosynthesis proceeds through decarboxylation via PS decarboxylase (Nerlich et al., 2007), the phosphoethanolamine transfer from CDP-ethanolamine to diacylglycerol (Kennedy pathway), and the exchange of the head group of PE with Ser via a base-exchange enzyme (Marshall and Kates, 1973). In particular, PS containing a relatively large amount of VLCFAs is enriched in endoplasmic reticulum (ER)-derived vesicles that may function in stabilizing small (70- to 80-nm-diameter) vesicles (Vincent et al., 2001).During the fatty acid elongation process, the first committed step is the condensation of C₂ units to acyl-CoA by KCS. Arabidopsis harbors a large family containing 21 KCS members (Joubès et al., 2008). Characterization of Arabidopsis KCS mutants with defects in VLCFA synthesis revealed in planta roles and substrate specificities (based on differences in carbon chain length and degree of unsaturation) of KCSs. For example, FAE1, a seed-specific condensing enzyme, was shown to catalyze C20 and C22 VLCFA biosynthesis for seed storage lipids (James et al., 1995). KCS6/CER6/CUT1 and KCS5/CER60 are involved in the elongation of fatty acyl-CoAs longer than C28 VLCFA for cuticular waxes in epidermis and pollen coat lipids (Millar et al., 1999; Fiebig et al., 2000; Hooker et al., 2002). KCS20 and KCS2/DAISY are functionally redundant in the two-carbon elongation to C22 VLCFA, which is required for cuticular wax and root suberin biosynthesis (Franke et al., 2009; Lee et al., 2009). When KCS1 and KCS9 were expressed in yeast (Saccharomyces cerevisiae), KCS1 showed broad substrate specificity for saturated and monounsaturated C16 to C24 acyl-CoAs and KCS9 utilized the C16 to C22 acyl-CoAs (Trenkamp et al., 2004; Blacklock and Jaworski, 2006; Paul et al., 2006). Recently, CER2 encoding putative BAHD acyltransferase was reported to be a fatty acid elongase that was involved in the elongation of C28 fatty acids for the synthesis of wax precursors (Haslam et al., 2012).In this study, the expression patterns and subcellular localization of KCS9 were examined, and an Arabidopsis kcs9 mutant was isolated to investigate the roles of KCS9 in planta. Diverse classes of lipids, including cuticular waxes, aliphatic suberins, and sphingolipids, as well as fatty acids in various organs were analyzed from the wild type, the kcs9 mutant, and complementation lines. The combined results of this study revealed that KCS9 is involved in the elongation of C22 to C24 fatty acids, which are essential precursors for the biosynthesis of cuticular waxes, aliphatic suberins, and membrane lipids, including sphingolipids. To the best of our knowledge, this is the first study where a KCS9 isoform involved in sphingolipid biosynthesis was identified.     

Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles

Engineering compositional changes in oilseeds is typically accomplished by introducing new enzymatic step(s) and/or by blocking or enhancing an existing enzymatic step(s) in a seed‐specific manner. However, in practice, the amounts of lipid species that accumulate in seeds are often different from what one would predict from enzyme expression levels, and these incongruences may be rooted in an incomplete understanding of the regulation of seed lipid metabolism at the cellular/tissue level. Here we show by mass spectrometry imaging approaches that triacylglycerols and their phospholipid precursors are distributed differently within cotyledons and the hypocotyl/radicle axis in embryos of the oilseed crop Camelina sativa, indicating tissue‐specific heterogeneity in triacylglycerol metabolism. Phosphatidylcholines and triacylglycerols enriched in linoleic acid (C18:2) were preferentially localized to the axis tissues, whereas lipid classes enriched in gadoleic acid (C20:1) were preferentially localized to the cotyledons. Manipulation of seed lipid compositions by heterologous over‐expression of an acyl–acyl carrier protein thioesterase, or by suppression of fatty acid desaturases and elongases, resulted in new overall seed storage lipid compositions with altered patterns of distribution of phospholipid and triacylglycerol in transgenic embryos. Our results reveal previously unknown differences in acyl lipid distribution in Camelina embryos, and suggest that this spatial heterogeneity may or may not be able to be changed effectively in transgenic seeds depending upon the targeted enzyme(s)/pathway(s). Further, these studies point to the importance of resolving the location of metabolites in addition to their quantities within plant tissues.