重组逆转录病毒浓缩方法研究

选择含有新霉素磷酸转移酶Ⅱ（Neor)的重组逆转录病毒载体,通过包装细胞进行包装,得到了含有重组逆转录病毒粒子的病毒溶液,该病毒溶液分别采用差速离心和滤膜截留两种方法进行浓缩,浓缩前,后的病毒溶液体外感染靶细胞NIH3T3以测定其滴度。结果显示,差速离心法的浓缩效率要优于滤膜截留法的浓缩效率,其浓缩效率能达到34．5％。     

水稻、大麦幼苗胚乳中的苯丙氨酸解氨酶调节因子(PAL-R)

从萌发的水稻、大麦胚乳中,通过85％硫酸铵盐析、DE52纤维素和Sephadex G100柱层析,获得部分纯化的苯丙氨酸解氨酶调节因子(PAL—R),研究其基本性质,及体外PAL-R对PAL和PAL-I的影响,表明它们之间存在着相互作用。     

浙江双栉蝠蛾发生与土壤的关系

土壤是影响浙江双栉蝠蛾发生的重要因子之一,本文通过对浙江双栉蝠蛾发生区土壤分析,明确了影响浙江双栉蝠蛾发生的土壤主要化学性质。发生严重的林分与轻度发生的林分相比,其土壤pH值小,速效K含量低,全N、全P含量高。因此,通过毛竹林地土壤管理及合理施肥可以减轻该虫的危害。     

开放的差异基因表达技术研究进展

自 90年代早期发展以来 ,差异基因表达 (DGE)技术在许多领域得到了应用 .“开放”结构系统的DGE技术不需原始的生物学或序列信息 ,而且可应用于任何种群 .主要介绍 6项开放的DGE技术 :cDNA代表性差示分析 (cDNA RDA)、基因表达系统分析 (SAGE)、表达序列标签串联排列连接(TALEST) ,和早期的DGE技术差异显示 (DD)、随机引物聚合酶链反应 (AP PCR) ,以及一项受专利保护的技术———GeneCalling .通过几项重要的参数对这些技术进行了比较 ,认为DD虽然有其致命的弱点 ,但在目前仍然应用得非常广泛 .cDNA RDA能有效富增特异片段 ,扣除共有序列 ,如能和SAGE结合 ,将能进一步促进其发展 .TALEST和GeneCalling操作较简便 ,一次试验能获得大量的数据 ,但是分析这些数据比较麻烦 ,须借助另外的分析软件 .最后介绍了应用DGE技术取得的最新成果 .     

Phylogeography of the marine macroalga Sargassum hemiphyllum (Phaeophyceae,Heterokontophyta) in northwestern Pacific

Sargassum hemiphyllum is commonly found in Japan and Korea, with a variety, var. chinense, that is found distributed in the southern Chinese coast. We previously reported distinct genetic differentiation between the two taxa based on the PCR‐RFLP data of plastid RubiscoL‐S spacer. The present study aims at elucidating the phylogeographic pattern of S. hemiphyllum based on more markers in the nuclear and extranuclear genomes, with a view to reveal the occurrence of hybridization. The two allopatrically distributed taxa were found to be genetically distinct in nuclear ITS2, plastidial Rubisco (Rbc) and mitochondrial TrnW_I (Trn) spacers. Their divergence was postulated to be attributable to the vicariant event which resulted from the isolation of the Sea of Japan during the late Miocene (6.58–11.25 Mya). Divergence within both S. hemiphyllum and the chinense variety was observed based on Trn spacer, while the divergence in S. hemiphyllum was further confirmed in Rbc spacer. This divergence appears to correspond to the separation of the Japanese populations between the Sea of Japan and the Pacific that occurred around 0.92–2.88 Mya (the early Pleistocene). The presence of an ITS2 clone resembling var. chinense sequences in a Japanese population of S. hemiphyllum (JpNS) raises the possibility of the introgression of var. chinense individuals into S. hemiphyllum population. Compared to that between S. hemiphyllum and the chinense variety, hybridization among the Japanese and Korean populations of S. hemiphyllum is highly probable as all these individuals share a pool of nuclear ITS2 sequences, possibly attributable to incomplete concerted evolution of ITS2.     

听神经瘤的显微外科现状

听神经瘤是主要起源于前庭神经的良性肿瘤,手术切除是其主要治疗方法之一,听神经瘤常用手术入路包括枕下乙状窦后入路(RSC)、经颅中窝入路(MFC)、经迷路入路(TLC)三种手术入路.非手术治疗包括放射治疗以及动态影像学观察.术中面神经监测应用为解决听神经瘤手术中面神经功能保留问题提供了一种很好的途径,使听神经瘤的并发症发生率已大大降低.本文就听神经瘤外科治疗做一综述.     

Systematic revision of the arboreal snail Satsuma albida species complex (Mollusca: Camaenidae) with descriptions of 14 new species from Taiwan

The taxonomy of the endemic arboreal snail Satsuma albida species complex from Taiwan was unclear due to the animals' highly similar morphology, and their nocturnal and strict arboreal behaviour, leading to difficulties in collecting living specimens. This article is the first comprehensive comparative study on the systematics and taxonomy of this species complex using external morphology, anatomy of the reproductive system and molecular phylogeny. Consequently, two subspecies of S. albida are raised to species status, namely S. insignis and S. mollicula. Fourteen new species are also described. Fourteen of the 17 species showed polymorphism in banding pattern amongst populations and other species retained the whitish unity as seen in S. albida. Distributions of almost all taxa are geographically limited, with the exception of S. polymorpha sp. nov . The phylogeny of these species was reconstructed using 20 morphological characters and molecular data from the partial sequences of mtDNA CO1 and 16S rRNA genes, and the complete ITS2 sequence. The molecular phylogeny revealed three subclades (west, east and polymorpha clade) and revealed that these snails are monophyletic, originating from a ground‐dwelling ancestor. © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society, 2008, 154 , 437–493.     

前交通动脉复合体的显微外科解剖研究

目的:通过手术显微镜对前交通动脉复合体及穿支进行解剖和测量,进一步熟悉和掌握该复合体的结构及毗邻血管的走行,为前交通动脉瘤手术提供解剖学依据.方法:用红色乳胶经颈内动脉对15例(30侧)福尔马林固定的湿性尸头进行灌注,然后在手术显微镜下对前交通动脉复合体进行解剖观测,所得结果用SPSS17.0软件进行统计分析.测量大脑前动脉A1和A2段、前交通动脉、Heubner回返动脉、A1段和前交通动脉穿支的长度、直径和各种形态变异.结果:未经选择的标本双侧A1发育无明显差异;术中对Heubner回返动脉、A1段穿支、前交通动脉穿支应仔细分辨加以保护;A1中1/3段穿支少,可作为前交通动脉瘤手术时临时阻断A1的部位;血管造影时前交通动脉不易看清与多种因素有关.结论:前交通动脉复合体复杂多变,熟悉前交通动脉复合体及穿支的解剖特点,对外科医生处理该区疾病至关重要.

Abstract：

Objective:To investigate the anatomy structure and adjacent vessels by investigating the anterior communicating artery complex and its perforating branches under an surgery microscope, in order to, to provide anatomical datas for anterior communicating aneurysms. Method: A total of 15 adult cadaveric heads (30 cerebral hemispheres) fixed with formalin were used, red latex was injected into internal carotid artery. Anterior communicating artery complexes were dissected, detected by surgery microscope.The results were analyzed with SPSS 17.0 statistical software. The lengthes, diameters and variations of the anterior cerebral artery(ACA)A 1 segment, A2 segment, anterior communicating artery (AComA), Heubner recurrent artery(HRA), perforating branches of A1 segment and AcomA were measured. Result:There were no statistically significant differences between two sides of A1 segment in length and diameter. HRA, perforating branches of Al segment and AComA should be carefully recognized and protected during the operation on anterior communicating aneurysms. The middle 1/3 of A1 segment which had less perforating branches was the best position for temporary occlusion during the operation on anterior communicating aneurysms. It is difficult to distinguish AComA in cerebral angiography. Conclusion: The anterior communicating artery complex was regarded as the most complex. Being familiar with the anatomy of the anterior communicating artery complex and the perforating branches enabled neurosurgeons to handle the diseases in this area efficiently.