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81.
V. B. Ivanov M. J. Bloemink P. A. Cheltsov E. I. Bystrova T. N. Fedotova J. Reedijk 《Biometals》1996,9(3):249-257
The amine substituent effect in compounds [Pt(diamine)Cl2] on inhibition of maize and cucumber root growth and branching has been investigated. The diamines used were ethylenediamine (en),N-methylethylenediamine (men),N,N-dimethylethylenediamine (N,N-dmen),N,N-dimethylethylenediamine (N,N-dmen),N,N,N,N-tetramethylethylenediamine (tmen), 1,2-propanediamine (1,2-pn), 2-methyl-1,2-propanediamine (ibn), 2,3 dimethyl-2,3-butanediamine (C-tmen), 1,3-propanediamine (1,3-pn), 2,2-dimethyl-1,3-propanediamine (C2-dm-1,3 pn),N,N-dimethyl-1,3-propanediamine (N,N-dm-1,3-pn). Increased substitution of hydrogen atoms of the amine part with CH3 groups reduces the cytostatic activity of complexes. The substitution of hydrogen atoms of NH2 and vicinal CH2 groups displays similar results. C-2 dimethylation (C-dm-1,3-pn) does not change the activity of the complex compared with (1,3-pn). It was observed that maize and cucumber roots differ in their relative sensitivity to various complexes. All complexes containing pn and their substituted analogs inhibited cucumber root growth weaker than that in maize. A comparison of obtained data with earlier published results concerning antitumor activity of complexes shows that they correlate in a similar manner with increased substitution of amino groups. Therefore, roots may be used as cheap test objects for primary screening of cytostatics. The general tendency of a decrease in cytostatic activity goes parallel with the number of Nor vicinal C-methyl groups and seems to arise from a decrease in hydrogen-bonding potential; however, some other possible reasons are also discussed. The activity discrimination by different species in our experiments and clearly different results forN,N-dimethylation depending on the chelate ring size (en and pn derivatives) on maize cannot be attributed to slower ligand-exchange kinetics from methylation. It is possible to assume that the major role in cytostatic activity of platinum complexes belongs to a cell repair system, i.e. the ability to eliminate platinum diamine fragments from DNA, depending on the number and strength of hydrogen bonds formed by thecis-diamine fragment. 相似文献
82.
R. A. Romanov A. A. Khokhlov M. F. Bystrova O. A. Rogachevskaja Yu. E. Yatzenko S. S. Kolesnikov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2007,1(3):240-245
Adenosine triphosphate (ATP) is a neurotransmitter/neuromodulator in both central and peripheral nervous systems. Particularly in the taste bud, a peripheral taste organ, ATP serves as an afferent neurotransmitter. To examine the mechanism that mediates ATP secretion in taste cells, we elaborated an approach for monitoring ATP in an extracellular medium by employing a biosensor, that is, cells responsive to ATP. Two lines of ATP-sensitive cells, HEK-293 and COS-1, which endogenously express P2Y receptors, were employed. In addition, HEK-293 cells transfected with P2X3 receptors were also used. By most relevant parameters (threshold response, inactivation kinetics of ATP responses, and refractory period), COS-1 cells were more suitable as an ATP sensor than HEK-293 cells, both native and transfected. For the HEK-293 cell-based biosensor, one of pitfalls was that they were highly responsive to mechanical disturbances, e.g., solution flux elicited by application of a chemical stimulus, owing to the expression of mechanosensitive Ca2+-permeable cation channels. In COS-1 cells, ATP-dependent Ca2+ transients were generated mostly due to Ca2+ release, the feature allowing one to control the activity of ATP-releasing cells electrophysiologically and to monitor the ATP secretion by Ca2+ responses of the ATP-biosensor. By using this technique, it was demonstrated that individual taste cells of a mouse released ATP in response to membrane depolarization. 相似文献
83.
Romanov RA Rogachevskaja OA Bystrova MF Jiang P Margolskee RF Kolesnikov SS 《The EMBO journal》2007,26(3):657-667
In mammalian taste buds, ionotropic P2X receptors operate in gustatory nerve endings to mediate afferent inputs. Thus, ATP secretion represents a key aspect of taste transduction. Here, we characterized individual vallate taste cells electrophysiologically and assayed their secretion of ATP with a biosensor. Among electrophysiologically distinguishable taste cells, a population was found that released ATP in a manner that was Ca(2+) independent but voltage-dependent. Data from physiological and pharmacological experiments suggested that ATP was released from taste cells via specific channels, likely to be connexin or pannexin hemichannels. A small fraction of ATP-secreting taste cells responded to bitter compounds, indicating that they express taste receptors, their G-protein-coupled and downstream transduction elements. Single cell RT-PCR revealed that ATP-secreting taste cells expressed gustducin, TRPM5, PLCbeta2, multiple connexins and pannexin 1. Altogether, our data indicate that tastant-responsive taste cells release the neurotransmitter ATP via a non-exocytotic mechanism dependent upon the generation of an action potential. 相似文献
84.
Peroxiredoxins (Prxs) are antioxidant proteins with peroxidase and chaperone activities. We assessed the self-association ability of 1-Cys Prx from rat olfactory epithelium. In native PAGE, the recombinant Prx-His6 produced in E. coli gave two bands corresponding to monomeric (minor) and dimeric (major) protein; incubation of the rPrx with DTT added a third band of oligomers. Western blotting of rat olfactory epithelium proteins resolved by native PAGE (with antiserum to rPrx) revealed that at physiological concentrations the 1-Cys Prx is mostly monomeric and to a lesser extent dimeric. This protein proved highly prone to aggregation in the presence of imidazole. 相似文献
85.
Onion (Allium cepa) is an important horticulture crop because of its value as a food with a long shelf life being a relatively non-perishable product. It is very helpful to understand the growth response of the seeded onion crop to conduct appropriate field practices in attaining the highest or optimum yields. A three year field experiment was conducted using a variety of onion Valcatorce INTA, in a randomized block design with five replicates. Treatments were two plant densities and three rates of N application. The bulb growth followed a classical sigmoid curve. During the rapid growing period, the crop had the greatest leaf area (LA) with at least six leaves per plant. Increasing plant density increased yield in kg/ha, but decreased bulb size. Defoliating 40 to 60% of the LA had a significant impact on bulb production only at early growth stages. Late in the growing period, the remaining LA was apparently large enough for producing sufficient amounts of metabolites to feed new leaves, increasing their photosynthesis efficiency for the benefit of bulb production. 相似文献
86.
The antimesometrial part of rat's decidua of 8-9 day of gestation was divided into three parts: epithelial, transition and basal zones. Cells of either zone have their own morphological and cytochemical features. Cells of the epithelial zone are characterized by synthesis of tissue specific antigens and fat (fatty acids). Cells of the transition zone synthesize glycogen. Acid mucopolysaccharides are located in the basal zone only. Decidual cells do not synthesize cholesterol. Con A receptors are localized on the surface of cells of the basal and transition zones and disappear from the surface of epithelial zone cells. It is concluded that differentiation of large decidual cells of the antimesometrial part of rat's decidua are accompanied by a significant change in cytochemical features of cell precursors the synthesis of acid mucopolysaccharides and glycogen stops, while tissue specific antigens and fat (fatty acids) start their syntheses, Con A receptor disappear. 相似文献
87.
A S Vladyko S I Bystrova N N Lemeshko I S Lukashevich 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1987,(5):37-40
Some properties of monoclonal antibodies to the Lassa virus have been characterized. The competitive immunoenzyme analysis has revealed the presence of at least three antigens in the Lassa virus nucleoprotein. 相似文献
88.
E A Selivanov V N Shabalin I M Bystrova I E Molokovskaia V I Rugal' 《Biulleten' eksperimental'no? biologii i meditsiny》1989,107(4):488-490
The study of chronic toxicity of polymerized hemoglobin solutions (PHb) has demonstrated the relationship between the dose of the solution administered and the level of functional and morphological changes induced in various organs of experimental animals. Lower doses (1.8 g/kg) of PHb had no effect on the values of protein and carbohydrate metabolism, while the high doses (10-15 g/kg) of PHb resulted in the impairment of protein synthesis, morphological changes in the spleen and lymphatic nodes. 相似文献
89.
Zhukovskaya N. V. Bystrova E. I. Lunkova N. F. Ivanov V. B. 《Russian Journal of Plant Physiology》2020,67(4):618-625
Russian Journal of Plant Physiology - Analysis of root growth parameters was carried out on the seedlings of 53 monocotyledonous and 78 dicotyledonous plant species. Daily increases of the... 相似文献
90.
Effects of heavy metals and strontium on division of root cap cells and meristem structural organization 总被引:4,自引:0,他引:4
A. D. Kozhevnikova I. V. Seregin E. I. Bystrova V. B. Ivanov 《Russian Journal of Plant Physiology》2007,54(2):257-266
In order to define relations between the behavior of quiescent center cells and the condition of root cap cells, effects of various metal salts on the root meristem structure, root growth, and division of root cap cells were investigated. Two-day-old maize (Zea mays L., cv. Diamant) seedlings were incubated on solutions containing 35 μM Ni(NO3)2), 10 μM Pb(NO3)2, or 3 mM Sr(NO3)2 in the absence or in the presence of 3 mM Ca(NO3)2. Toxic effects of metals were assessed from inhibition of the primary root length increment following 24-h and 48-h incubations as compared to the roots grown on water or on 3 mM Ca(NO3)2 solution. Metal localization in the root apex tissues following 24-h and 48-h incubations was determined using histochemical techniques. Cell lengths in three upper layers of root cap columella were determined, and the mitotic index in these cells was calculated. In the absence of Ca(NO3)2, the metals were found both in the meristem and in the root cap. Pb and Sr were revealed primarily in the cell walls, and Ni, in the cell protoplasts. In the presence of Ca(NO3)2, metal content in all root tissues was decreased, and their toxic effect on root growth was ameliorated. Pb and Ni inhibited cell division in the root cap. Pb caused an increase in the root cap cell length as early as following 24-h incubation, and Ni, only following 48-h incubation. Pb activated division of quiescent center cells in the direction of root cap. These effects, as well as possible involvement of dermatogen and cortex cells, resulted in a regrowth of a new root cap already after a 24-h incubation period. In this case, the meristem was transformed from a closed structure into the open one. Following 48-h incubation, Ni brought about only few divisions of quiescent center cells in the direction of root cap. It was suggested that inhibition of divisions of the root cap upper layer cells and a decrease in the sloughing off its cells can stimulate the quiescent center cell divisions. A similarity of the quiescent center and animal stem cells is discussed. 相似文献