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41.
Anaerobic respiration in the Rhodospirillaceae: characterisation of pathways and evaluation of roles in redox balancing during photosynthesis 总被引:2,自引:0,他引:2
Abstract Recent discoveries relating to pathways of anaerobic electron transport in the Rhodospirillaceae are reviewed. The main emphasis is on the organism Rhodobacter capsulatus ** but comparisons are made with Rhodobacter sphaeroides ** f. sp. denitrificans and Rhodopseudomonas palustris . The known electron acceptors for anaerobic respiration in Rhodobacter capsulatus are trimethylamine- N -oxide (TMAO), dimethyl sulphoxide (DMSO), nitrate and nitrous oxide. In each case respiration generates a proton electrochemical gradient and in some cases can support growth on non-fermentable carbon sources. However, the principal objective of this review is to discuss the possibility that, apart from a role in energy conservation, anaerobic respiration in the photosynthetic bacteria may have a special function in maintaining redox balance during photosynthetic metabolism. Thus the electron acceptors mentioned above may serve as auxiliary oxidants: (a) to maintain an optimal redox poise of the photosynthetic electron transport chain; (b) to provide a sink for electrons during phototrophic growth on highly reduced carbon substrates.
Molecular properties of the nitrate reductase, nitrous oxide reductase and a single enzyme responsible for reduction of TMAO and DMSO are discussed. These enzymes are all located in the periplasm. Electrons destined for all three enzymes can originate from the rotenone-sensitive NADH dehydrogenase but do not proceed through the antimycin- and myxothiazol-sensitive cytochrome b/c1 complex. It is likely, therefor, that the pathways of anaerobic respiration overlap with the cyclic photosynthetic electron transport chain only at the level of the ubiquinone pool. Redox components which might be involved in the terminal branches of anaerobic respiration are discussed. 相似文献
Molecular properties of the nitrate reductase, nitrous oxide reductase and a single enzyme responsible for reduction of TMAO and DMSO are discussed. These enzymes are all located in the periplasm. Electrons destined for all three enzymes can originate from the rotenone-sensitive NADH dehydrogenase but do not proceed through the antimycin- and myxothiazol-sensitive cytochrome b/c
42.
Bryan D. Noe Sharon L. Milgram A. Balasubramaniam P. C. Andrews Jaroslaw Calka John K. McDonald 《Cell and tissue research》1989,257(2):303-311
Summary Results from a previous report demonstrate that more than one molecular form of neuropeptide Y-like peptide may be present in the islet organ of the anglerfish (Lophius americanus). Most of the neuropeptide Y-like immunoreactive material was anglerfish peptide YG, which is expressed in a subset of islet cells, whereas an additional neuropeptide Y-like peptide(s) was localized in islet nerves. To learn more about the neuropeptide Y-like peptides in islet nerves, we have employed immunohistochemical and biochemical methods to compare peptides found in anglerfish islets and brain. Using antisera that selectively react with either mammalian forms of neuropeptide Y or with anglerfish peptide YG, subsets of neurons were found in the brain that labelled with only one or the other of the antisera. In separate sections, other neurons that were labelled with either antiserum exhibited similar morphologies. Peptides from brains and islets were subjected to gel filtration and reverse-phase high performance liquid chromatography. Radioimmunoassays employing either the neuropeptide Y or peptide YG antisera were used to examine chromatographic eluates. Immunoreactive peptides having retention times of human neuropeptide Y and porcine neuropeptide Y were identified in extracts of both brain and islets. This indicates that peptides structurally similar to both of these peptides from the neuropeptide Y-pancreatic polypeptide family are expressed in neurons of anglerfish brain and nerve fibers of anglerfish islets. The predominant form of neuropeptide Y-like peptide in islets was anglerfish peptide YG. Neuropeptide Y-immunoreactive peptides from islet extracts that had chromatographic retention times identical to human neuropeptide Y and porcine neuropeptide Y were present in much smaller quantities. These results are consistent with the hypothesis that peptides having significant sequence homology with human neuropeptide Y and porcine neuropeptide Y are present in the nerve fibers that permeate the islet. 相似文献
43.
Stuart K 《Parasitology today (Personal ed.)》1989,5(1):5-8
44.
Anna L. Ballard Alastair G. McEwan David J. Richardson J. Baz Jackson Stuart J. Ferguson 《Archives of microbiology》1990,154(3):301-303
Rhodobacter capsulatus strain BK5 possesses a membrane bound respiratory nitrate reductase rather than the periplasmic enzyme found in other strains. The enzyme in strain BK5 is shown to be both functionally and structurally related to the nitrate reductase of Paracoccus denitrificans and Escherichia coli.Abbreviation TMAO
trimethylamine-N-oxide 相似文献
45.
Summary Phosphinothricin is a non-selective herbicide which inhibits glutamine synthetase (EC 6.3.1.2) activity causing an overaccumulation of ammonia in higher plants. Alfalfa (Medicago sativa L) shoot tissue and petiole-derived callus exposed to phosphinothricin show 50 and 70% reductions, respectively, in glutamine synthetase activity with a concomitant rise of 10 and 20 fold, respectively, in endogenous ammonia. The diffusibility of ammonia may limit the use of a detoxifying gene, phosphinothricin acetyltransferase, as a selectable marker for alfalfa transformation. However, the addition of up to 40 times the standard levels of ammonium nitrate to the culture media used in this study had no effect on callus growth, although glutamine synthetase activity was inhibited by 50% and endogenous ammonia increased 27 fold. Therefore, ammonia accumulation may not be the primary cause of cell death in alfalfa after exposure to phosphinothricin. It follows that diffusion of ammonia from cell to cell would not restrict the selection for phosphinothricin acetyltransferase transformed cells, thereby indicating that this enzyme could be used as a selectable marker in transformation experiments.Abbreviations PPT
Phosphinothricin
- PAT
Phosphinothricin acetyltransferase 相似文献
46.
47.
Homozygous osteogenesis imperfecta unlinked to collagen I genes 总被引:4,自引:1,他引:3
Katherine Aitchison Donald Ogilvie Mary Honeyman Elizabeth Thompson Bryan Sykes 《Human genetics》1988,78(3):233-236
Summary In a consanguineous pedigree in which a severe type of osteogenesis imperfecta was segregating as an autosomal recessive trait, analysis of genetic markers for both collagen I structural loci COL1A1 and COL1A2 showed that the phenotype was unlinked to either locus. 相似文献
48.
49.
The growth rate of a population of Euphausia lucens from thewest coast of South Africa was estimated from laboratory studiesand from monthly size-frequency distributions of samples collectedover a 1-year period. Laboratory studies indicated that growthrates ranged from 0.131 (larvae) to 0.047 mm day1 (juveniles),while size-frequency distributions suggested a growth rate of{small tilde}0.026 mm day1 for the adults. The mean annualbiomass from the inshore, intermediate and offshore regionsranged from 9.75 to 47.29 mg dry wt m3 with the highestbiomass being found in the inshore region. Calyptopis larvaewere present for most months of the year, indicating continuousrecrwtment. The relative contribution of flesh, moults and eggsto the total annual production was estimated separately forall three regions. Production due to growth (Pg) was estimatedto be 92.71185.60 mg dry wt m3 year1, whileexuviai production (Pe) varied between 60.01 and 281.38 mg drywt m year Production of eggs (Pr) was estimated to range from5.07 to 12.39 mg dry wt m year the lowest value being obtainedin the inshore region. Moult production represented {small tilde}6times the mean biomass in each region, while the P/B ratio forflesh production varied from 3.92 to 8.91, the highest ratiobeing obtained in the offshore region. Total P/B ratios rangedfrom 10.14 to 16.01. 相似文献
50.
Effect of nitrogen stress and abscisic acid on nitrate absorption and transport in barley and tomato
F. Stuart Chapin III David T. Clarkson John R. Lenton Colin H. S. Walter 《Planta》1988,173(3):340-351
The potential of barley (Hordeum vulgare L.) and tomato (Lycopersicon esculentum Mill.) roots for net NO
3
-
absorption increased two-to five fold within 2 d of being deprived of NO
3
-
supply. Nitrogen-starved barley roots continued to maintain a high potential for NO
3
-
absorption, whereas NO
3
-
absorption by tomato roots declined below control levels after 10 d of N starvation. When placed in a 0.2 mM NO
3
-
solution, roots of both species transported more NO
3
-
and total solutes to the xylem after 2 d of N starvation than did N-sufficient controls. However, replenishment of root NO
3
-
stores took precedence over NO
3
-
transport to the xylem. Consequently, as N stress became more severe, transport of NO
3
-
and total solutes to the xylem declined, relative to controls. Nitrogen stress caused an increase in hydraulic conductance (L
p) and exudate volume (J
v) in barley but decrased these parameters in tomato. Nitrogen stress had no significant effect upon abscisic acid (ABA) levels in roots of barley or flacca (a low-ABA mutant) tomato, but prevented an agerelated decline in ABA in wild-type tomato roots. Applied ABA had the same effect upon barley and upon the wild type and flacca tomatoes: L
p and J
v were increased, but NO
3
-
absorption and NO
3
-
flux to the xylem were either unaffected or sometimes inhibited. We conclude that ABA is not directly involved in the normal changes in NO
3
-
absorption and transport that occur with N stress in barley and tomato, because (1) the root ABA level was either unaffected by N stress (barley and flacca tomato) or changed, after the greatest changes in NO
3
-
absorption and transport and L
p had been observed (wild-type tomato); (2) changes in NO
3
-
absorption/transport characteristics either did not respond to applied ABA, or, if they did, they changed in the direction opposite to that predicted from changes in root ABA with N stress; and (3) the flacca tomato (which produces very little ABA in response to N stress) responded to N stress with very similar changes in NO
3
-
transport to those observed in the wild type.Abbreviation and symbols ABA
abscisic acid
- Jv
exudate volume
- Lp
root hydraulic conductance 相似文献