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971.
A.M.M. Reis S.D. Araújo Jr R.L. Moura R.B. Francini-Filho G. Pappas Jr A.M.A. Coelho R.H. Krüger F.L. Thompson 《Journal of applied microbiology》2009,106(4):1378-1387
Aims: We performed the first characterization of the microbiota associated with the reef coral Mussismilia braziliensis by means of a culture-independent approach.
Methods and Results: The main groups were Proteobacteria , Cyanobacteria and unclassified bacteria according to the 16S rDNA libraries. Most of the sequences of the mucus of healthy and diseased M. braziliensis did not find close matches in GenBank (i.e. >97% 16S rDNA similarity). Most of the sequences of seawater and mucus of healthy coral fell into tight clusters (17 and 15 clusters respectively). In contrast, most of the sequences of mucus of diseased coral did not form clusters. The rarefaction curves indicate saturation in the recovery of higher taxa (approximately 40 phyla). However, the number of species in the coral mucus ( n = 130–170) and seawater ( n = 170) did not reach a plateau.
Conclusions: The coral microbiota encompasses several potentially novel species and higher taxa. The microbiota of M. braziliensis appears to be species-specific. Diseased coral may have provided a suitable place for colonization by opportunistic bacteria, resulting in a greater bacterial diversity.
Significance and Impact of the Study: The first study on the diversity of the microbiota of the endemic and endangered of extinction coral M. braziliensis . 相似文献
Methods and Results: The main groups were Proteobacteria , Cyanobacteria and unclassified bacteria according to the 16S rDNA libraries. Most of the sequences of the mucus of healthy and diseased M. braziliensis did not find close matches in GenBank (i.e. >97% 16S rDNA similarity). Most of the sequences of seawater and mucus of healthy coral fell into tight clusters (17 and 15 clusters respectively). In contrast, most of the sequences of mucus of diseased coral did not form clusters. The rarefaction curves indicate saturation in the recovery of higher taxa (approximately 40 phyla). However, the number of species in the coral mucus ( n = 130–170) and seawater ( n = 170) did not reach a plateau.
Conclusions: The coral microbiota encompasses several potentially novel species and higher taxa. The microbiota of M. braziliensis appears to be species-specific. Diseased coral may have provided a suitable place for colonization by opportunistic bacteria, resulting in a greater bacterial diversity.
Significance and Impact of the Study: The first study on the diversity of the microbiota of the endemic and endangered of extinction coral M. braziliensis . 相似文献
972.
Amorim EP Vilarinhos AD Cohen KO Amorim VB Dos Santos-Serejo JA Silva SO Pestana KN Dos Santos VJ Paes NS Monte DC Dos Reis RV 《Genetics and molecular biology》2009,32(1):96-103
The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 μg.g (-1) , and ranged from 1.06 μg.g (-1) for the triploid Nanica (Cavendish group) to 19.24 μg.g (-1) for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 μg.g (-1)). The mean similarity among the 42 accessions was 0.63 (range: 0.24 to 1.00). DArT analysis revealed extensive genetic variability in accessions from the Embrapa Musa germplasm bank. 相似文献
973.
Bruna Barneda-Zahonero Alfredo Mi?ano-Molina Nahuai Badiola Rut Fadó Xavier Xifró Carlos A. Saura José Rodríguez-Alvarez 《Molecular biology of the cell》2009,20(24):5051-5063
Bone morphogenetic proteins (BMPs) have been implicated in the generation and postnatal differentiation of cerebellar granule cells (CGCs). Here, we examined the eventual role of BMPs on the survival of these neurons. Lack of depolarization causes CGC death by apoptosis in vivo, a phenomenon that is mimicked in vitro by deprivation of high potassium in cultured CGCs. We have found that BMP-6, but not BMP-7, is able to block low potassium–mediated apoptosis in CGCs. The neuroprotective effect of BMP-6 is not accompanied by an increase of Smad translocation to the nucleus, suggesting that the canonical pathway is not involved. By contrast, activation of the MEK/ERK/CREB pathway by BMP-6 is necessary for its neuroprotective effect, which involves inhibition of caspase activity and an increase in Bcl-2 protein levels. Other pathways involved in the regulation of CGC survival, such as the c-Jun terminal kinase and the phosphatidylinositol 3-kinase (PI3K)-Akt/PKB, were not affected by BMP-6. Moreover, failure of BMP-7 to activate the MEK/ERK/CREB pathway could explain its inability to protect CGCs from low potassium–mediated apoptosis. Thus, this study demonstrates that BMP-6 acting through the noncanonical MEK/ERK/CREB pathway plays a crucial role on CGC survival. 相似文献
974.
Claudilson J. C. Bastos Roque Aras Gildo Mota Francisco Reis Juarez Pereira Dias Robson Silva de Jesus Miralba Silva Freire Eline G. de Araújo Juliana Prazeres Maria Fernanda Rios Grassi 《PLoS neglected tropical diseases》2010,4(6)
Background
Outbreaks of orally transmitted Trypanosoma cruzi continue to be reported in Brazil and are associated with a high mortality rate, mainly due to myocarditis.Methods
This study is a detailed report on the disease progression of acute Chagas disease in 13 patients who were infected during two micro-outbreaks in two northeastern Brazilian towns. Clinical outcomes as well as EKG and ECHO results are described, both before and after benznidazole treatment.Results
Fever and dyspnea were the most frequent symptoms observed. Other clinical findings included myalgia, periorbital edema, headache and systolic murmur. Two patients died of cardiac failure before receiving benznidazole treatment. EKG and ECHO findings frequently showed a disturbance in ventricular repolarization and pericardial effusion. Ventricular dysfunction (ejection fraction <55%) was present in 27.3% of patients. After treatment, EKG readings normalized in 91.7% of patients. Ventricular repolarization abnormalities persisted in 50% of the patients, while sinus bradycardia was observed in 18%. The systolic ejection fraction normalized in two out of three patients with initially depressed ventricular function, while pericardial effusion disappeared.Conclusions
Myocarditis is frequently found and potentially severe in patients with acute Chagas disease. Benznidazole treatment may improve clinical symptoms, as well as EKG and ECHO findings. 相似文献975.
Samuele Peppoloni Susanna Ricci Carlotta F. Orsi Bruna Colombari Maria Margherita De Santi Massimino Messinò Giuliana Fabio Alessio Zanardi Elena Righi Velia Braione Sergio Tripodi Damiana Chiavolini Marcella Cintorino Michele Zoli Marco Rinaldo Oggioni Elisabetta Blasi Gianni Pozzi 《Microbes and infection / Institut Pasteur》2010,12(12-13):990-1001
The polysaccharide capsule is a major virulence factor of Streptococcus pneumoniae as it confers resistance to phagocytosis. The encapsulated serotype 4 TIGR4 strain was shown to be efficiently phagocytosed by the mouse microglial cell line BV2, whereas the type 3 HB565 strain resisted phagocytosis. Comparing survival after uptake of TIGR4 or its unencapsulated derivative FP23 in gentamicin protection and phagolysosome maturation assays, it was shown that TIGR4 was protected from intracellular killing. Pneumococcal capsular genes were up-regulated in intracellular TIGR4 bacteria recovered from microglial cells. Actual presence of bacteria inside BV2 cells was confirmed by transmission electron microscopy (TEM) for both TIGR4 and FP23 strains, but typical phagosomes/phagolysosomes were detected only in cells infected with the unencapsulated strain. In a mouse model of meningitis based on intracranic inoculation of pneumococci, TIGR4 caused lethal meningitis with an LD50 of 2 × 102 CFU, whereas the LD50 for the unencapsulated FP23 was greater than 107 CFU. Phagocytosis of TIGR4 by microglia was also demonstrated by TEM and immunohistochemistry on brain samples from infected mice. The results indicate that encapsulation does not protect the TIGR4 strain from phagocytosis by microglia, while it affords resistance to intracellular killing. 相似文献
976.
Mauro de Freitas Ortiz Karina Rodrigues Lorenzatto Bruna Renata Silva Corrêa Elgion Lucio Silva Loreto 《Genetica》2010,138(6):649-655
Transposable elements (TEs) comprise a significant fraction of the genome, and some models of the TE “life cycle” suggest
that, in the last phases of the cycle, TEs should be represented, in the genomes, by inactive and degenerated copies. In this
study, we analyzed, using a bioinformatics approach, the autonomous hAT elements and their derivatives (active non-autonomous, MITE relatives and degenerated copies) in 12 Drosophila genomes. We found 28 hAT elements that had derivatives. Most copies had features that suggested that they were active, while only a few degenerated
copies were found. Because hAT elements comprise an evolutionarily old superfamily, one should expect to find many degenerated copies within the genome,
although this was not observed in our study. These results suggest that primarily active copies of hAT elements are maintained in the euchromatic regions of the genome and that degenerated copies are removed from the genome
by natural selection. 相似文献
977.
978.
The spatial distribution of insects is essential to perform control strategies, to improve sample techniques and to estimate economic losses. We aimed to determine the spatial distribution of nests of Acromyrmex crassispinus (Forel) in Pinus taeda plantations. The experiments were carried out in P. taeda plantations with different ages (treatments: recently-planted, three and six-year old plants). The study took place in Rio Negrinho and in Três Barras, SC. Three plots of one hectare were delimited in each treatment, and plots were divided in 64 sample units. The analysis of the dispersion index [variance/mean relationship (I), index of Morisita (Iδ) and k exponent of negative binomial distribution] showed that the majority of the samplings presented random distribution. Among the three distributions of probabilities studied: Poisson, positive binomial and negative binomial, the Poisson distribution was the best model to fit the spatial distribution of A. crassispinus nests in all samplings. The result was a random distribution in the plantings of different ages. 相似文献
979.
Secondary forests are an increasingly common feature in tropical landscapes worldwide and understanding their regeneration is necessary to design effective restoration strategies. It has previously been shown that the woody species community in secondary forests can follow different successional pathways according to the nature of past human activities in the area, yet little is known about patterns of herbaceous species diversity in secondary forests with different histories of land use. We compared the diversity and abundance of herbaceous plant communities in two types of Central Amazonian secondary forests—those regenerating on pastures created by felling and burning trees and those where trees were felled only. We also tested if plant density and species richness in secondary forests are related to proximity to primary forest. In comparison with primary forest sites, forests regenerating on non‐burned habitats had lower herbaceous plant density and species richness than those on burned ones. However, species composition and abundance in non‐burned stands were more similar to those of primary forest, whereas several secondary forest specialist species were found in burned stands. In both non‐burned and burned forests, distance from the forest edge was not related to herbaceous density and species richness. Overall, our results suggest that the natural regeneration of herbaceous species in secondary tropical forests is dependent on a site's post‐clearing treatment. We recommend evaluating the land history of a site prior to developing and implementing a restoration strategy, as this will influence the biological template on which restoration efforts are overlaid. 相似文献
980.
Denis Broock Rosemberg Eduardo Pacheco Rico Andrei Silveira Langoni Jonathan Tesch Spinelli Talita Carneiro Pereira Renato Dutra Dias Diogo Onofre Souza Carla Denise Bonan Maurício Reis Bogo 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2010,155(3):230-240
The nucleoside triphosphate diphosphohydrolase (NTPDase) family cleaves tri- and diphosphonucleosides to monophosphonucleosides and is responsible for terminating purinergic transmission. Since the NTPDase family in zebrafish is poorly understood, here we evaluated the nucleotide hydrolysis in three tissues of adult zebrafish (brain, liver, and heart), confirmed the presence of distinct NTPDase members by a phylogenetic analysis and verified their relative gene expression profiles in the respective tissues. A different profile of ATP and ADP hydrolysis in the brain, liver, and heart as a function of time and protein concentration was observed. Sodium azide (20 mM), ARL 67156 (300 μM) and Suramin (300 μM) differently altered the nucleotide hydrolysis in zebrafish tissues, suggesting the contribution of distinct NTPDase activities. Homology-based searches identified the presence of NTPDase1-6 and NTPDase8 orthologs and the phylogeny also grouped three NTPDase2 and two NTPDase5 paralogs. The deduced amino acid sequences share the apyrase conserved regions, conserved cysteine residues, putative N-glycosylation, phosphorylation, N-acetylation sites, and different numbers of transmembrane domains. RT-PCR experiments revealed the existence of a distinct relative entpd1-6 and entpd8 expression profile in brain, liver, and heart. Taken together, these results indicate that several NTPDase members might contribute to a tight regulation of nucleotide hydrolysis in zebrafish tissues. 相似文献