全文获取类型
收费全文 | 452篇 |
免费 | 20篇 |
国内免费 | 8篇 |
出版年
2022年 | 8篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2015年 | 10篇 |
2014年 | 7篇 |
2013年 | 7篇 |
2012年 | 11篇 |
2011年 | 14篇 |
2010年 | 11篇 |
2009年 | 17篇 |
2008年 | 23篇 |
2007年 | 13篇 |
2006年 | 12篇 |
2005年 | 6篇 |
2004年 | 11篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 12篇 |
2000年 | 3篇 |
1999年 | 12篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 11篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1989年 | 8篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1983年 | 5篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1974年 | 3篇 |
1959年 | 7篇 |
1958年 | 23篇 |
1957年 | 26篇 |
1956年 | 26篇 |
1955年 | 22篇 |
1954年 | 22篇 |
1953年 | 13篇 |
1952年 | 13篇 |
1951年 | 10篇 |
1950年 | 9篇 |
1949年 | 2篇 |
1948年 | 2篇 |
1912年 | 2篇 |
排序方式: 共有480条查询结果,搜索用时 15 毫秒
101.
102.
103.
Lars A. Brudvig 《植被学杂志》2016,27(1):4-5
Landscape connectivity is considered a key factor regulating species diversity of natural and managed landscapes, yet the influence of connectivity relative to other factors remains poorly resolved. In this issue of Journal of Vegetation Science, Jakobsson and colleagues illustrate how grassland plant species richness is more strongly affected by the age and management of grassland patches than by connectivity. 相似文献
104.
A new in vitro model has been developed for studying migration of human polymorphonuclear leukocytes (PMN) through living native cellular and matrix barriers. Human amnion membrane consists of a single layer of epithelium bound to a continuous basement membrane interfacing an avascular collagenous stroma. Living amnion was placed in plastic chambers with separate compartments on each side of the membrane. PMN were introduced on the epithelial side of the amnion, and a Millipore filter (Millipore Corp., Bedford, Mass.) was placed against the stromal side. In response to N-formylmethionyl-leucyl- phenylanlanine (FMLP) chemoattractant, PMN penetrated the full thickness of the amnion and were collected and counted on the filter. The rate of PMN traversal of the amnion was dependent on the concentration of FMLP (optimal at 10(-8)M) as well as the slope of the FMLP gradient across the amnion. The route of PMN migration was studied by transmission electron microscopy. PMN first attached to the epithelial surface, then infiltrated between intercellular junctions. PMN migrated around or through tight junction and hemidesmosome attachments. The PMN then penetrated the basement membrane and migrated through the dense collagenous stroma. The present amnion migration system has characteristics of the in vivo inflammatory state not described in any previous method for monitoring PMN migration in vitro. Prior methods have not used native epithelium, whole basement membrane, or collagenous stroma. PMN penetration of these barriers occurs in the normal inflammatory response and probably involves biochemical mechanisms not required for simple migration through the pores of an artificial filter. The amnion system can be useful for future biochemical and morphological studies of PMN penetration of these barriers and possible repair processes that may follow. 相似文献
105.
106.
107.
Evidence for the absence of photoreduction of the metal centers of cytochrome C oxidase by X-irradiation 总被引:1,自引:0,他引:1
Samples of X-irradiated cytochrome c oxidase were examined by electron paramagnetic resonance and optical spectroscopy. Both radiation from the Stanford Synchrotron Radiation Laboratory and a conventional X-ray source (W target) were utilized. The X-ray flux from these sources ranges from 10(9) to 10(13) photons/s. No evidence was found for photoreduction of the metal centers in the enzyme by X-ray photons. These results demonstrate that the integrity of cytochrome c oxidase is maintained using the conditions under which X-ray absorption measurements are presently being made. 相似文献
108.
109.
Chung-Ling Lu Steven Ortmeier Jon Brudvig Tamara Moretti Jacob Cain Simeon A. Boyadjiev Jill M. Weimer Jinoh Kim 《Traffic (Copenhagen, Denmark)》2022,23(1):81-93
SEC24 is mainly involved in cargo sorting during COPII vesicle assembly. There are four SEC24 paralogs (A–D) in vertebrates, which are classified into two subgroups (SEC24A/B and SEC24C/D). Pathological mutations in SEC24D cause osteogenesis imperfecta with craniofacial dysplasia in humans. sec24d mutant fish also recapitulate the phenotypes. Consistent with the skeletal phenotypes, the secretion of collagen was severely defective in mutant fish, emphasizing the importance of SEC24D in collagen secretion. However, SEC24D patient-derived fibroblasts show only a mild secretion phenotype, suggesting tissue-specificity in the secretion process. Using Sec24d KO mice and cultured cells, we show that SEC24A and SEC24B also contribute to endoplasmic reticulum (ER) export of procollagen. In contrast, fibronectin 1 requires either SEC24C or SEC24D for ER export. On the basis of our results, we propose that procollagen interacts with multiple SEC24 paralogs for efficient export from the ER, and that this is the basis for tissue-specific phenotypes resulting from SEC24 paralog deficiency. 相似文献
110.
Ecological restoration can reverse biodiversity loss worldwide, yet restoration goals and outcomes vary widely, which limits this potential. Divergent restoration outcomes may stem from variation in conditions at the outset of restoration, but empirical evidence is lacking and typically confounded with site differences. Additionally, precipitation is usually cited as the source of this variation, although a wide range of conditions can vary annually. We tested for effects of planting year on seedling establishment by installing identical restorations in three different years. Within those years, we manipulated rainfall with rain‐out shelters to disentangle the effects of precipitation from other annually variable conditions. Additionally, we tested whether increasing seed mix richness buffers against adverse planting conditions. For the first growing season after planting, we followed emergence and survival of sown prairie species and nonsown weed species to determine how planting year conditions influence an establishing plant community, if at all. We found that seedling establishment differed across planting years and precipitation treatments, and that varying emergence patterns by species led to differences in the composition of the first‐year community. We also found significant variation in sown species establishment across years when precipitation was held constant, illustrating the previously overlooked role of nonprecipitation drivers on planting year effects. Higher seed mix richness did not consistently improve establishment of sown species under different planting conditions. This research provides important experimental evidence for effects of interannual variation in planting conditions on first‐year establishment. Future work will examine how these initial changes affect longer‐term assembly dynamics. 相似文献