Dengue virus-specific cross-reactive CD8+ human cytotoxic T lymphocytes

Stimulation with live dengue virus of peripheral blood mononuclear cells from a dengue virus type 4-immune donor generated virus-specific, serotype-cross-reactive, CD8+, class I-restricted cytotoxic T lymphocytes (CTL) capable of lysing dengue virus-infected cells and cells pulsed with dengue virus antigens of all four serotypes. These CTL lysed autologous fibroblasts infected with vaccinia virus-dengue virus recombinant viruses containing the E gene or several nonstructural dengue virus type 4 genes. These results demonstrate that both dengue virus structural and nonstructural proteins are targets for the cytotoxic T-cell-mediated immune response to dengue virus and suggest that serotype-cross-reactive CD8+ CTL may be important mediators of viral clearance and of virus-induced immunopathology during secondary dengue virus infections.     

A review of the genus Parahemiurus Vaz & Pereira, 1930 (Digenea: Hemiuridae)

The genus Parahemiurus Vaz & Pereira, 1930 (syn.: Daniella Sahai & Srivastava, 1977) is defined, its major morphological characters discussed and a key to species given. The species P. merus (Linton, 1910) (syns: P. parahemiurus Vaz & Pereira, 1930, P. sardiniae Yamaguti, 1934, P. seriolae Yamaguti, 1934, P. platichthyi Lloyd, 1938, P. atherinae Yamaguti, 1938, P. harengulae Yamaguti, 1938, P. noblei King, 1962) and P. anchoviae Pereira & Vaz, 1930 are described. Other species recognized are P. clupeae Yamaguti, 1953, P. [originally Daniella] madrasensis (Sahai & Srivastava, 1977) n. comb. (syns: P. dussumieriai Hafeezullah, 1981, P. indicus Ahmad, 1981), P. ecuadori Manter, 1940, P. engraulisi Gupta & Jahan, 1977 (syns: P. cameroni Gupta & Ahmad, 1977, P. puriensis Ahmad, 1981, P. simhai Gupta & Gupta, 1978, P. tricanthusi Gupta & Puri, 1984) and P. yanamense Hafeezullah, 1980. Forms considered species inquirendae are P. arripidis Lebedev, 1971, P. clupeae of King (1964), P. dogieli Skrjabin & Guschanskaya, 1953, P. pseudosciaenae Shen, 1985 and P. trachichthodi Lebedev, 1968. Host and locality information is given in detail for all species. The complete life-cycle is not known, but metacercariae are reported in chaetognaths and teleosts. The definitive hosts of Parahemiurus spp. most frequently reported belong in the families Clupeidae and Carangidae and the genus is most commonly reported in temperate and subtropical waters.     

A lea-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae

During periods of water deficit, plants accumulate late embryogenesis-abundant (LEA) proteins which are thought to protect cells from stresses associated with dehydration. One of these genes, le25, is expressed in tomato leaves and roots in response to water deficit and abscisic acid accumulation. To study the function of this protein and to test the effect of overproduction of the LE25 protein in Saccharomyces cerevisiae (Sc), a recombinant plasmid in which le25 is expressed under the control of the GAL1 promoter was constructed. The content of LE25 was high in Sc cells transformed with the recombinant plasmid. The transformant exhibited several stress-tolerant phenotypes. Growth of the transformant in a medium with 1.2 M NaCl was improved, as compared to a control strain. While the control strain showed a long lag phase of 40 h, le25-expressing cells showed a shortened lag phase of 10 h. However, no growth improvement was observed in a medium with 2 M sorbitol. In addition, the transformant had an increased survival rate after freezing stress, but not after high-temperature stress. These results, together with its predicted secondary structure, may indicate that LE25 functions as an ion scavenger.     

A redescription ofLepidauchen stenostoma Nicoll, 1913 (Digenea), and a reassessment of the status of the genusLepidauchen Nicoll, 1913

The genusLepidauchen is discussed and defined. It is removed from the family Lepocreadiidae and placed in the family Acanthocolpidae based mainly on its possession of a uterine seminal receptacle. The type, and only valid species,L. stenostoma, is redescribed using material fromLabrus merula from Corsica. Material fromCoris julis andSymphodus tinca from the western Mediterranean off Marseille,L. merula from the western Mediterranean off Valencia,Diplodus annularis andLithognathus mormyrus from the Adriatic Sea off Montenegro, andArchosargus unimaculatus from Venezuela was also examined. Other nominal species are discussed, but none are considered valid within this genus.     

Relative apparent synapomorphy analysis (RASA). I: The statistical measurement of phylogenetic signal

We have developed a new approach to the measurement of phylogenetic signal in character state matrices called relative apparent synapomorphy analysis (RASA). RASA provides a deterministic, statistical measure of natural cladistic hierarchy (phylogenetic signal) in character state matrices. The method works by determining whether a measure of the rate of increase of cladistic similarity among pairs of taxa as a function of phenetic similarity is greater than a null equiprobable rate of increase. Our investigation of the utility and limitations of RASA using simulated and bacteriophage T7 data sets indicates that the method has numerous advantages over existing measures of signal. A first advantage is computational efficiency. A second advantage is that RASA employs known methods of statistical inference, providing measurable sensitivity and power. The performance of RASA is examined under various conditions of branching evolution as the number of characters, character states per character, and mutations per branch length are varied. RASA appears to provide an unbiased and reliable measure of phylogenetic signal, and the general approach promises to be useful in the development of new techniques that should increase the rigor and reliability of phylogenetic estimates.      

Topical Fat Reduction

The fat on women's thighs is more difficult to mobilize due to increased α-2 adrenergic receptor activity induced by estrogen. Lipolysis can be initiated through adipocyte receptor stimulation (β adrenergic) or inhibition (adenosine or α-2 adrenergic) or by inhibition of phosphodiesterase. Since many women desire regional thigh fat loss, a series of clinical trials were initiated using one thigh as a double-blinded control. Trial #1: Five overweight women had injections of isoproterenol at intervals around the thigh three times a week for 4 weeks with diet and walking. Trial #2: Five overweight woman had ointment containing forskolin, yohimbine and aminophylline applied to the thigh five times a week for 4 weeks after hypertonic warm soaks with a diet and walking. Trial #3: Eighteen overweight women were divided into three groups of six and trial #2 was repeated with each agent alone vs. placebo using forskolin, yohimbine or aminophylline in separate ointments. Trial #4: Thirty overweight women had 10% aminophylline ointment applied to the thigh five times a week for 6 weeks with diet and walking. Chemistry panel, theophylline level and patch testing wereperformed. Trial#5: Twelve women had trial #4 repeated with 2% aminophylline cream without a diet or walking. Trial #6: Trial #5 was repeated with 0.5% aminophylline cream. All trials except yohimbine ointment gave significantly more girth loss from the treated thigh (p < 0.05 to p < 0.001). Chemistry panel showed no toxicity. Theophylline was undetectable and patch testing was negative. We conclude that topical fat reduction for women's thighs can be achieved without diet or exercise.     

Evaluation of Drugs for Treating Obesity

Criteria for the evaluation of new drugs to treat obesity are important as guides for designing clinical trials to test these agents. These criteria must be developed in relation to the realities of obesity, which is a chronic disease associated with morbidity and mortality that is increased by visceral fat deposits. The observation that patients regain weight after stopping drug treatment for obesity argues for the proposition that drugs work only when taken and NOT that the drugs are ineffective. The analogy between the development of treatments for obesity to those for the treatment of hypertension is used to highlight potential areas for new developments. Several features of an ideal drug for the treatment of obesity are suggested. Criteria for evaluating new drugs include both primary and secondary endpoints. The primary endpoint for an anti-obesity drug should be weight loss, possibly by category of success. Losses of total body fat or visceral fat might be alternative primary endpoints. Secondary endpoints include reduction in risk factors for associated diseases and improvement in the quality of life. In trials where vigorous placebo designs including highly aggressive behavior modification or very-low-calorie diets were used, it may be difficult or impossible to detect a response to a drug.     

A review of the Tandanicolinae (=Monodhelminthinae) (Digenea: Fellodistomidae), with a description of Prosogonarium angelae n. sp.

The Tandanicolinae is reviewed, redefined and considered a subfamily of the Fellodistomidae. The Monodhelminthinae Dollfus, 1937, Mehratrematinae Srivastava, 1939, Prosogonariinae Mehra, 1963 and Buckleytrematinae Yamaguti, 1971 are synonyms of the Tandanicolinae. Genera included in the Tandanicolinae are Tandanicola, Monodhelmis, Buckleytrema and Prosogonarium. Burnellus and Mehratrema are made new synonyms of Monodhelmis. The composition of each genus is reviewed. Monodhelmis arii Yamaguti, 1952, Mehratrema arii Gu & Shen, 1979, Mehratrema dollfusi Srivastava, 1939, Monodhelmis philippinensis Velasquez, 1961, Mehratrema polynemusinis Chauhan, 1943 and Mehratrema skrjabini Karyakarte, 1969 are considered synonyms of Monodhelmis torpedinis. Monodhelmis elongatus Bilqees, 1970 is considered a species inquirenda. Prosogonarium angelae n. sp. is described from Euristhmus lepturus from Moreton Bay, Queensland. It is distinguished by the number and distribution of the vitelline follicles and the extent of the caeca. New records are given for Monodhelmis torpedinis from Arius graeffei and E. lepturus, M. trichofurcata from Tandanus tandanus, and Buckleytrema indica from A. graeffei, all from southern Queensland. Buckleytrema indica is redescribed and B. postacetabulorchis is synonymised with that species. Relationships within the Tandanicolinae were considered by cladistic analysis.     

Regeneration dynamics of alluvial gaps in a warm temperate rain forest in New Zealand

Gaps formed by stream laid gravels in a warm temperate Podocarp-Angiosperm rain forest in New Zealand were quickly invaded by 41 free-standing woody species. Height-age curves for the eight most frequent species were used to construct a 12 yr chronosequence which showed 18 species arrived by 0.9 yr and 33 by 1.9 yr. This rapid invasion was supplied from both gaps and mature forest and fitted the Initial Floristic Model coupled with a Facilitation Mechanism. Over the 12 yr period, stem density and births peaked at 0.9 yr and deaths at 1.9 yr with a natality of 39.5/m², a mortality of 38.5/m² and nearly 99% dying as seedlings. Plant height increased to 5.1 m at 12.1 yr with height increment peaking at 43 cm/yr at 7.3 yr. Maximum potential heights ranged from 4 to 60 m and were positively correlated with longevity which varied from 20 to 1150 yr for 19 known species. A decline in height increment to 0.9 yr showed a below-ground component in intraspecific competition with significant negative correlations between stem density and height indicating a later above-ground component. There was no significant height competition amongst the 11 most frequent species and only one significant negative interspecific association. This suggests high species coexistence for which Equal chance was probably the major mechanism. Given this coexistence, rapid species arrival, early sexual maturity and similar density and height-age patterns, the main dynamic variables may be maximum height, longevity and height increment, all of which are, partly, genetically programmed. Most dynamic affinities are with tropical forest, although height growth and species richness are warm temperate.