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排序方式: 共有180条查询结果,搜索用时 62 毫秒
21.
I. Toumi M. Gargouri I. Nouairi P. N. Moschou A. Ben Salem-Fnayou A. Mliki M. Zarrouk A. Ghorbel 《Biologia Plantarum》2008,52(1):161-164
To dissect differences in both lipid accumulation and composition and the role of these modifications during drought stress,
four grapevine cultivars exhibiting differential tolerance to drought were subjected to water shortage. Tolerant cultivars,
Kahli Kerkennah and Cardinal, exhibited higher leaf water potential (Ψw), and lower lipid peroxidation compared to the sensitive cultivars Guelb Sardouk and Superior Seedless during stress. Total
lipid amounts increased during stress only in the leaves of the tolerant cultivars. Drought induced increases in the ratios
digalactosyldiacylglycerol/monogalactosyldiacylglycerol and phosphatidylcholine/phoshatidylethanolamine of almost all the
drought stressed cultivars. Moreover, the overall analysis of the composition of fatty acids revealed that a linolenic acid
was prevalent in grapevine and the unsaturation level of lipids increased under water stress in all the cultivars. Specific
adjustments in the lipid composition during stress could compromise stress tolerance. 相似文献
22.
The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU ml−1 filter paper activity, 21 IU ml−1 CMCases activity (endoglucanase units) and 25 mg ml−1 of proteins. Various tests were done to compare the action of the P. occitanis cellulases with those commercially available and with the traditional stonewashing process. This cellulase preparation was successfully applied in a biostoning process at an industrial scale. The abrasive effect of the P. occitanis cellulases was very uniform and with an efficiency comparable to that obtained by the commercial ones. 相似文献
23.
Based on light and scanning electron microscopical studies, two new gonad-infecting species of Philometra Costa, 1845, P. draco n. sp. and P. radiata n. sp. (Nematoda: Philometridae), are described from the marine perciform fishes Trachinus draco (Linnaeus) and T. radiatus (Linnaeus) (both Trachinidae), respectively, in the Gulf of Hammamet, off the northeastern coast of Tunisia. Philometra draco n. sp. and P. radiata n. sp. can be separated from other gonad-infecting species of this genus by the structures associated to the gubernaculum (e.g. dorsal protuberance, smooth field separating the dorsolateral longitudinal parts), as well as by the length of the body, spicules and gubernaculum. Philometra radiata n. sp. can be distinguished from P. draco n. sp. in having the dorsal side of the gubernaculum distal end provided with a median longitudinal smooth field demarcated by two dorsolateral lamellate parts. These two new species are the first philometrid species described from fishes of the family Trachinidae. 相似文献
24.
M. Aloulou-Abdelkefi H. Trigui-Lahiani A. Gargouri 《Applied Biochemistry and Microbiology》2017,53(2):230-236
The production of hydrolytic enzymes by the mutant Trichoderma reesei Rut-C30 when cultivated in the presence of various carbon sources: glucose, wheat bran and autoclaved mycelium of Penicillium occitanis CT1 has been studied. Glucose was shown to repress all studied hydrolases, 3% of either wheat bran or autoclaved cell walls led to high titers of enzymes, and were favorably comparable to commercial lysing enzymes (LE). The lysing enzyme cocktail obtained when T. reesei Rut-C30 was cultivated in the presence of autoclaved P. occitanis CT1 mycelia appeared to be a most effective for P. occitanis CT1 protoplast formation. Maximal yield of protoplasts reached 13 × 106 protoplasts/mL while commercial LE preparation released only 4 × 106 protoplasts/mL. The protoplast yield was affected also by the osmotic stabilizer, with KCl giving the best results. Our results suggest that to achieve the best protoplastization rate, the enzyme preparation should be obtained following induction by the autoclaved mycelium of the autologous fungus. 相似文献
25.
Korey J. Brownstein Mahmoud Gargouri William R. Folk David R. Gang 《Metabolomics : Official journal of the Metabolomic Society》2017,13(11):133
Introduction
Botanicals containing iridoid and phenylethanoid/phenylpropanoid glycosides are used worldwide for the treatment of inflammatory musculoskeletal conditions that are primary causes of human years lived with disability, such as arthritis and lower back pain.Objectives
We report the analysis of candidate anti-inflammatory metabolites of several endemic Scrophularia species and Verbascum thapsus used medicinally by peoples of North America.Methods
Leaves, stems, and roots were analyzed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) was performed in MetaboAnalyst 3.0 after processing the datasets in Progenesis QI.Results
Comparison of the datasets revealed significant and differential accumulation of iridoid and phenylethanoid/phenylpropanoid glycosides in the tissues of the endemic Scrophularia species and Verbascum thapsus.Conclusions
Our investigation identified several species of pharmacological interest as good sources for harpagoside and other important anti-inflammatory metabolites.26.
27.
Olfa Frikha-Gargouri Radhouane Gdoura Abir Znazen Boutheina Gargouri Jalel Gargouri Ahmed Rebai Adnene Hammami 《BMC microbiology》2008,8(1):217
Background
The OmcB protein is one of the most immunogenic proteins in C. trachomatis and C. pneumoniae infections. This protein is highly conserved leading to serum cross reactivity between the various chlamydial species. Since previous studies based on recombinant proteins failed to identify a species specific immune response against the OmcB protein, this study evaluated an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of C. trachomatis infections. 相似文献28.
Lobna Gargouri Kammoun Samia Gargouri Mohamed Rabeh Hajlaoui Mohamed Marrakchi 《Journal of Phytopathology》2009,157(9):546-551
An outbreak of Fusarium Head Blight of durum wheat occurred in 2004 being localized in sub-humid and higher semi-arid region of Northern Tunisia. A mycological survey carried out throughout these regions, revealed that 78% of the prospected fields were infested. Results of the morphological and molecular identification, showed that the most common species isolated from diseased wheat spikes was Microdochium nivale var. nivale (63.5%), followed by Fusarium culmorum (26%), F. pseudograminearum (9%) and F. avenaceum (1.5%). To evaluate mycotoxin content of naturally infected grain, the amounts of trichothecene mycotoxin deoxynivalenol (DON) in harvested grain from 45 fields were quantified by RIDASCREEN DON Enzyme Immunoassay Kit (ELISA) . This study showed that the infection levels in freshly harvested grain were very low and the maximum deoxynivalenol (DON) level of the positive samples was 53 ppb. This is the first report on the natural occurrence of DON in naturally infected wheat grain sampled from Northern Tunisia. 相似文献
29.
S. Krid A. Rhouma J.M. Quesada R. Penyalver A. Gargouri 《Journal of applied microbiology》2009,106(3):886-894
Aims: To investigate the genetic diversity of Pseudomonas savastanoi pv. savastanoi strains and to look whether these strains were distributed to geographical location.
Methods and Results: Random amplification of polymorphic DNA (RAPD) was used to discriminate between 58 Tunisian strains and 21 strains from various other countries of P. savastanoi pv. savastanoi , the causal agent of olive knot disease. Isolates were separated into three groups by cluster analysis and principal coordinate analysis of RAPD fingerprint data obtained with three primers (OPR-12, OPX-7 and OPX-14). Group 1 contained isolates from the southeast of Tunisia and European strains. Group 2 comprised strains isolated from the north of Tunisia exclusively while group 3 encompassed the majority of isolates obtained from five orchards located in the centre of Tunisia.
Conclusions: The results indicated that isolates of P. savastanoi pv. savastanoi were genetically distinct according to geographic regions. RAPD grouped isolates derived from the same orchard as identical.
Significance and Impact of the Study: This is the first application of RAPD in the delineation of P. savastanoi pv. savastanoi strains. 相似文献
Methods and Results: Random amplification of polymorphic DNA (RAPD) was used to discriminate between 58 Tunisian strains and 21 strains from various other countries of P. savastanoi pv. savastanoi , the causal agent of olive knot disease. Isolates were separated into three groups by cluster analysis and principal coordinate analysis of RAPD fingerprint data obtained with three primers (OPR-12, OPX-7 and OPX-14). Group 1 contained isolates from the southeast of Tunisia and European strains. Group 2 comprised strains isolated from the north of Tunisia exclusively while group 3 encompassed the majority of isolates obtained from five orchards located in the centre of Tunisia.
Conclusions: The results indicated that isolates of P. savastanoi pv. savastanoi were genetically distinct according to geographic regions. RAPD grouped isolates derived from the same orchard as identical.
Significance and Impact of the Study: This is the first application of RAPD in the delineation of P. savastanoi pv. savastanoi strains. 相似文献
30.
Fatma Trifa Sondes Karray-Chouayekh Imed Mabrouk Sami Baccouche Abdelmajid Khabir Tahia Sellami-Boudawara Ali Gargouri Raja Mokdad-Gargouri 《Cancer epidemiology》2010,34(2):184-188
Background: The p53 polymorphisms have been extensively studied as putative breast cancer susceptibility variants. The present study was undertaken to investigate the association of p53 Arg72Pro, Ins16bp and G13964C polymorphisms and their haplotypes with breast cancer risk in Tunisian women. Methods: Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on 159 patients and 132 controls. Results: The G13964C intronic variant was significantly associated with familial breast cancer risk (p = 0.0018) while the genotypic distribution was similar for p53 Arg72Pro and Ins16bp in patients and controls. Moreover, the (NoIns-C), (Arg-C) and (NoIns-Arg-C) haplotypes were significantly associated with familial breast cancer risk (p = 0.0021, p = 0.0096 and p = 0.0084, respectively) while there was a trend of association between the (Ins-Arg) and (Ins-Arg-G) haplotypes and the risk of sporadic breast cancer. Only the G/C genotype as well as the (NoIns-C) haplotype remained significant after correction for multiple testing. Conclusion: Our data revealed an association between the G/C genotype and the (NoIns-C) haplotype and the risk of familial breast cancer in Tunisian women. However, these observations need to be confirmed due to the limited statistical power of our study and the small number of cases. 相似文献