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Mohamed Badaoui Najjar Dimitri Kashtanov Michael L. Chikindas 《Probiotics and antimicrobial proteins》2009,1(2):143-147
The present study aims to examine the efficacy of the natural antimicrobial ε-poly-l-lysine against Streptococcus mutans and against total aerobic oral microflora, alone and in combination with the natural antimicrobial peptide nisin. In in vitro studies, natural antimicrobials nisin A and ε-poly-l-lysine synergized in their action against S. mutans, leading to the microorganism’s full inhibition, while having a less inhibitory effect on total aerobic oral microbiota. 相似文献
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Ballut L Petit F Mouzeyar S Le Gall O Candresse T Schmid P Nicolas P Badaoui S 《Biochimica et biophysica acta》2003,1645(1):30-39
Proteasomes have been purified from sunflower hypocotyles. They elute with a molecular mass of 600 kDa from gel filtration columns and two-dimensional gel electrophoresis indicates that the complex contains at least 20 different protein subunits. Peptide microsequencing revealed the presence of four subunits homologous to subunits Beta2, Beta6, Alpha5 and Alpha6 of plant proteasomes. These proteasomes have chymotrypsin-like activity and the highly purified fraction of this complex is associated with an endonuclease activity hydrolyzing Tobacco mosaic virus RNA and Lettuce mosaic virus RNA with a cleavage pattern showing fragments of well-defined size. This is the first evidence of a RNA endonuclease activity associated with plant proteasomes. 相似文献
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Gautier-Bert K Murol B Jarrousse AS Ballut L Badaoui S Petit F Schmid HP 《Molecular biology reports》2003,30(1):1-7
We have partially reconstituted 20S proteasome/RNA complexes using oligonucleotides corresponding to ARE (adenosine- and uridine-rich element) (AUUUA)4 and HIV-TAR (human immunodeficiency virus-Tat transactivation response element), a stem-loop structure in the 5 UTR (untranslated region) of HIV-mRNAs. We demonstrate that these RNAs which associate with proteasomes are degraded by proteasomal endonuclease activity. The formation of these 20S proteasome/RNA substrate complexes is rather specific since 20S proteasomes do not interfere with truncated TAR that is not cleaved by proteasomal endonuclease. In addition, affinity of proteasomes for (AUUUA)4 is much stronger as it is for HIV-TAR. These results provide further arguments for our hypothesis that proteasomes could be involved in the destabilisation of cytokines mRNAs containing AUUUA sequences as well as viral mRNAs. 相似文献
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L. Gentzbittel S. Mouzeyar S. Badaoui E. Mestries F. Vear D. Tourvieille De Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):519-525
A candidate-gene approach to analyse the resistance of plants to phytopathogenic fungi is presented. The resistance of sunflower
(Helianthus annuus L.) to downy mildew (Plasmopara halstedii) shows a gene-for-gene interaction (monogenic resistance), whereas resistance to white rot (Sclerotinia sclerotiorum) is quantitative, with different levels of resistance for different plant parts. By homology cloning, probes were obtained
homologous to some plant resistance genes (nucleotide binding site-like, NBS, genes and serine-threonine protein kinase-like,
PK, genes). These clones were used as probes for linkage mapping of the corresponding genes. It was demonstrated that at least
three NBS-like loci are located on linkage-group 1, in the region where downy mildew resistance loci have been described.
Quantitative trait loci for S. sclerotiorum resistance to penetration or extension of the mycelium in different tissues were studied in three crosses. Major QTLs for
resistance were found on linkage group 1, with up to 50% of the phenotypic variability explained by peaks at the map position
of the PK locus, 25 cM from the downy mildew loci.
Received: 24 September 1997 / Accepted: 21 October 1997 相似文献
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L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
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Jarrousse Anne-Sophie Gautier Karine Apcher Sébastien Badaoui Saloua Boissonnet Gérard Dadet Marie-hélène Henry Laurent Bureau Jean-Paul Schmid Hans-Peter Petit Franck 《Molecular biology reports》1999,26(1-2):113-117
The interrelationships between proteasomes and viral gene products are very complex. 20S proteasomes associate with a number of viral mRNAs which are cleaved by proteasome's associated endonuclease activity. In addition proteasome's endopeptidase activities are involved in the presentation of viral antigens. Viral proteins of different origin associate with the 20S and 26S complexes and interfere with their enzymatic activities. A major part of this review deals with the interactions between 20S proteasomes and the gene products of the human immunodeficiency virus (HIV) which has been studied in detail by our group. 相似文献
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Badaoui B D'Andrea M Pilla F Capote J Zidi A Jordana J Ferrando A Delgado JV Martínez A Vidal O Amills M 《Biochemical genetics》2011,49(7-8):523-532
Agouti signaling protein (ASIP) is one of the key players in the modulation of hair pigmentation in mammals. Binding to the melanocortin 1 receptor, ASIP induces the synthesis of phaeomelanin, associated with reddish brown, red, tan, and yellow coats. We have sequenced 2.8?kb of the goat ASIP gene in 48 individuals and identified two missense (Cys126Gly and Val128Gly) and two intronic polymorphisms. In silico analysis revealed that the Cys126Gly substitution may cause a structural change by disrupting a highly conserved disulfide bond. We studied its segregation in 12 Spanish and Italian goat breeds (N?=?360) with different pigmentation patterns and found striking differences in the frequency of the putative loss-of-function Gly(126) allele (Italian 0.43, Spanish Peninsular 0.08), but we did not observe a clear association with coat color. This suggests that the frequency of this putative loss-of-function allele has evolved under the influence of demographic rather than selection factors in goats from these two geographical areas. 相似文献
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Bouzidi MF Badaoui S Cambon F Vear F De Labrouhe DT Nicolas P Mouzeyar S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):592-600
Resistance of sunflower to the obligate parasite Plasmopara halstedii is conferred by specific dominant genes, denoted Pl. The Pl6 locus confers resistance to all races of P. halstedii except one, and must contain at least 11 tightly linked genes each giving resistance to different downy mildew races. Specific primers were designed and used to amplify 13 markers
covering a genetic distance of about 3 cM centred on the Pl6 locus. Cloning and sequence analysis of these 13 markers indicate that Pl6 contains conserved genes belonging to the TIR-NBS-LRR class of plant resistance genes.
Received: 9 April 2001 / Accepted: 10 August 2001 相似文献