Immunoglobulin superantigen protein L induces IL-4 and IL-13 secretion from human Fc epsilon RI+ cells through interaction with the kappa light chains of IgE

Peptostreptococcus magnus protein L is a multidomain bacterial surface protein that correlates with virulence. It consists of up to five homologous Ig-binding domains (B1-B5) that interact with the variable domain of Ig kappa L chains. Intact protein L stimulates the synthesis and the release of IL-4 and IL-13 from human basophils in vitro. A protein L fragment covering the Ig-binding domains B1-B4 also induced IL-4 and IL-13 release from basophils. There was an excellent correlation (r(s) = 0.82; p < 0.001) between the maximal percent IL-4 release induced by protein L and that induced by anti-IgE and between intact protein L and the B1-B4 fragment (r(s) = 0.90; p < 0.01). Removal of IgE bound to basophils markedly reduced the IL-4 release induced by anti-IgE, protein L, and B1-B4. Preincubation of basophils with protein L or anti-IgE caused complete cross-desensitization to subsequent challenge with the heterologous stimulus. IgE purified from myeloma patients PS and PP (lambda chains) blocked anti-IgE-induced IL-4 release, but not the releasing activity of protein L. In contrast, IgE purified from myeloma patient ADZ (kappa chains) blocked both anti-IgE- and protein L-induced secretion. Cyclosporin A, but not cyclosporin H, inhibited protein L-induced release of IL-4 and IL-13 from basophils. Thus, protein L acts as a bacterial Ig superantigen to induce the synthesis and release of IL-4 and IL-13 from basophils by interacting with kappa L chains of the IgE isotype.     

alpha-amylase from five strains of Bacillus amyloliquefaciens: evidence for identical primary structures.

The alpha-amylases from five strains of Bacillus amyloliquefaciens were compared to determine whether differences in primary structure are responsible for variations in catalytic properties previously reported among the enzymes. Amino acid analysis established virtually identical compositions for the proteins. Reaction with dimethylaminoaphthylene sulfonylchloride indicated the amino-terminal amino acid of each amylase to be valine. Carboxyl termini of the enzymes have been determined by digestion with carboxypeptidase A. The resulting kinetic data indicate tyrosine as the carboxyl terminus and leucine as the penultimate residue for all five proteins. Isoelectric focusing of the enzymes yielded isoelectric points in the pH range of 5.09 to 5.18. Tryptic digests of the enzymes chromatographed on a cation-exchange column showed identical elution patterns. It is concluded that the primary structure of the amylase from the five strains is identical or exhibits only conservative substitutions.     

IEEE 802.11b Ad Hoc Networks: Performance Measurements

In this paper we investigate the performance of IEEE 802.11b ad hoc networks by means of an experimental study. An extensive literature, based on simulation studies, there exists on the performance of IEEE 802.11 ad hoc networks. Our analysis reveals several aspects that are usually neglected in previous simulation studies. Firstly, since different transmission rates are used for control and data frames, different transmission ranges and carrier-sensing ranges may exist at the same time in the network. In addition, the transmission ranges are in practice much shorter than usually assumed in simulation analysis, not constant but highly variable (even in the same session) and depends on several factors. Finally, the results presented in this paper indicate that for correctly understanding the behavior of an 802.11b network operating in ad hoc mode, several different ranges must be considered. In addition to the transmission range, the physical carrier sensing range is very important. The transmission range is highly dependent on the data rate and is up to 100 m, while the physical carrier sensing range is almost independent from the data rate and is approximately 200 m. Furthermore, even though stations are outside from their respective physical carrier sensing range, they may still interfere if their distance is lower than 350 m.Giuseppe Anastasi received the Laurea degree in Electronics Engineering and the Ph.D. degree in Computer Engineering both from the University of Pisa, Italy, in 1990 and 1995, respectively. He is currently an associate professor of Computer Engineering at the Department of Information Engineering of the University of Pisa. His research interests include architectures and protocols for mobile computing, energy management, QoS in mobile networks, and ad hoc networks. He was a co-editor of the book Advanced Lectures in Networking (LNCS 2497, Springer, 2002), and published more than 50 papers, both in international journals and conference proceedings, in the area of computer networking. He served in the TPC of several international conferences including IFIP Networking 2002 and IEEE PerCom 2003. He is a member of the IEEE Computer Society.Eleonora Borgia received the Laurea degree in Computer Engineering from the University of Pisa, Italy, in 2002. She is currently working toward her Ph.D. degree at the IIT Institute of the Italian National Research Council (CNR). Her research interests are in the area of the wireless and mobile networks with particular attention to MAC protocols and routing algorithms for ad hoc networks.Marco Conti received the Laurea degree in Computer Science from the University of Pisa, Italy, in 1987. In 1987 he joined the Italian National Research Council (CNR). He is currently a senior researcher at CNR-IIT. His research interests include Internet architecture and protocols, wireless networks and ad hoc networking, mobile computing, and QoS in packet switching networks. He co-authored the book Metropolitan Area Networks (Springer, London, 1997), and published in journal and conference proceedings more than 100 research papers related to design, modeling, and performance evaluation of computer-network architectures and protocols. He served as the technical program committee chair of the IFIP-TC6 conferences Networking 2002 and PWC 2003, and technical program committee co-chair of ACM WoWMoM 2002. He is serving as technical program committee co-chair of the IEEE Symposium on a World of Wireless, Mobile and Multimedia Networks (WoWMoM 2005). He served as guest editor for the Cluster Computing Journal (special issue on Mobile Ad Hoc Networking), IEEE Transactions on Computers (special issue on Quality of Service issues in Internet Web Services), and ACM/Kluwer Mobile Networks & Applications Journal (special issue on Mobile Ad hoc Networks). He is member of IFIP WGs 6.2, 6.3 and 6.8.Enrico Gregori received the Laurea in electronic engineering from the University of Pisa in 1980. He joined CNUCE, an institute of the Italian National Research Council (CNR) in 1981. He is currently a CNR research director. In 1986 he held a visiting position in the IBM research center in Zurich working on network software engineering and on heterogeneous networking. He has contributed to several national and international projects on computer networking. He has authored more than 100 papers in the area of computer networks and has published in international journals and conference proceedings and is co-author of the book Metropolitan Area Networks (Springer, London, 1997). He was the General Chair of the IFIP TC6 conferences: Networking2002 and PWC2003 (Personal Wireless Communications). He served as guest editor for the Networking2002 journal special issues on: Performance Evaluation, Cluster Computing and ACM/Kluwer Wireless Networks Journals. He is a member of the board of directors of the Create-Net association, an association with several Universities and research centres that is fostering research on networking at European level. He is on the editorial board of the Cluster Computing, of the Computer Networks and of the Wireless Networks Journals. His current research interests include: Wireless access to Internet, Wireless LANs, Quality of service in packet-switching networks, Energy saving protocols, Evolution of TCP/IP protocols.     

Toll-like receptor gene polymorphisms in patients with myeloproliferative neoplasms

Molecular Biology Reports - Toll-like receptors (TLRs) are a family of transmembrane receptors whose signaling control cellular processes of cell proliferation, survival, apoptosis, angiogenesis,...     

Plumage based classification of the bowerbird genus Sericulus evaluated using a multi-gene, multi-genome analysis

Past classifications of taxa within the bowerbird genus Sericulus (family: Ptilonorhynchidae) conflict since the discovery of hybrids identified though male plumage characteristics. We use molecular data to help define species within this genus, and by estimating a phylogeny, test for lability in the evolution of male plumage patterns. Because this genus includes the most brightly colored bowerbird species, and is hypothesized to be the basal genus of the avenue building bowerbird clade, the organization of the four taxa within this genus is especially important in understanding how bowerbird plumage coloration evolved. Analyses of two mitochondrial and six nuclear gene regions confirm the basal placement of Sericulus in the avenue building bowerbirds and Sericulus monophyly, and suggests the Australian S. chrysocephalus is the basal Sericulus species. Our analysis additionally supports the existence of three New Guinea Sericulus species, contrary to some previous plumage based classifications, as they are genetically equidistant from each other. Molecular and geographic data of New Guinea are consistent suggesting a series of speciation events starting approximately 3.7-4.3MYA leading to four extant Sericulus species. The absence of resolution within the New Guinea species precludes any statements of trait lability, but does suggest that traits under high selection pressures may not accurately indicate species level distinctions within this genus.     

Ultrastructural study of the mycelium-yeast transition in Histoplasma capsulatum. I. Changes at 37 degrees C

Ultrastructural changes observed during the first 24 hours of mycelium to yeast transition in the dimorphic fungus Histoplasma capsulatum are reported. During this period the plasma membrane becomes undulated and the cell wall loses its characteristic fibrous outer layer. At 8 h the ordered lamellar structure of the mitochondria is no longer apparent. 24 h after the temperature shift 70% of the cells are lysed. The remaining cells contain many cytoplasmic membrane structures; mitochondria are rarely observed. These morphological changes are probably correlated with the physiological events characteristic of mycelial to yeast transition.     

Female signals enhance the efficiency of mate assessment in satin bowerbirds (Ptilonorhynchus violaceus)

Recent evidence suggests that males adjust their sexually selecteddisplay traits in response to female behaviors during courtships.Little is known, however, about whether females signal to influencemale displays and whether females benefit from this interaction.Male courtship displays in the satin bowerbird (Ptilonorhynchusviolaceus) are highly intense and aggressive. Females may usethese displays as indicators of mating benefits, but these displaysoften startle females and disrupt courtship. Previous studieshave shown that successful males decrease female startling byadjusting their display intensity according to female crouchingbehaviors, suggesting that crouching behaviors function as signals.Here we address whether female crouching is a signal by usingobservations of natural courtship behaviors. In addition, weexamine why females differ in signaling and whether femalesbenefit from signaling. First, we find that female crouchingis related to the likelihood that females will be startled bymale displays, suggesting that crouching signals the degreeof display intensity that females will tolerate from a malewithout being startled. Second, we find that female tolerancefor intense display increases during successive courtships asfemales assess potential mates, and that female tolerance mayalso be affected by age and condition. Third, we find evidencethat females that reduce startling by signaling their intensitytolerance are more efficient in mate searching. These resultssuggest that females signal to influence how males display theirsexually selected traits, and by doing so, females may increasetheir benefits in mate choice.     

Purification of a soluble and a wall-bound form of beta-glucosidase from Mucor racemosus.

beta-Glucosidase activity in crude extracts of Mucor racemosus exists in a soluble form and in a wall-bound form which sediments at 3,500 x g. The soluble form and a wall-bound form were purified to homogeneity by ammonium sulfate fractionation. DEAE-Sephadex chromatography, and SP-Sephadex chromatography. Both forms were identical in all parameters measured. Each enzyme is a glycoprotein of 91,000 daltons, with an identical amino acid composition and N-terminal amino acid of lysine; both contain about 10% carbohydrate. Both forms catalyze the hydrolysis of cellobiose and p-nitrophenyl-beta-D-glucoside with identical kinetic constants.