全文获取类型
收费全文 | 2200篇 |
免费 | 210篇 |
国内免费 | 147篇 |
出版年
2023年 | 23篇 |
2022年 | 47篇 |
2021年 | 102篇 |
2020年 | 77篇 |
2019年 | 86篇 |
2018年 | 100篇 |
2017年 | 72篇 |
2016年 | 123篇 |
2015年 | 160篇 |
2014年 | 146篇 |
2013年 | 169篇 |
2012年 | 205篇 |
2011年 | 175篇 |
2010年 | 105篇 |
2009年 | 76篇 |
2008年 | 113篇 |
2007年 | 105篇 |
2006年 | 81篇 |
2005年 | 80篇 |
2004年 | 61篇 |
2003年 | 60篇 |
2002年 | 48篇 |
2001年 | 44篇 |
2000年 | 37篇 |
1999年 | 35篇 |
1998年 | 25篇 |
1997年 | 24篇 |
1996年 | 29篇 |
1995年 | 22篇 |
1994年 | 16篇 |
1993年 | 12篇 |
1992年 | 13篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 13篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1977年 | 2篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1965年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2557条查询结果,搜索用时 15 毫秒
101.
Identification of the active site of human mitochondrial malonyl‐coenzyme a decarboxylase: A combined computational study
下载免费PDF全文
![点击此处可从《Proteins》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Malonyl‐CoA decarboxylase (MCD) can control the level of malonyl‐CoA in cell through the decarboxylation of malonyl‐CoA to acetyl‐CoA, and plays an essential role in regulating fatty acid metabolism, thus it is a potential target for drug discovery. However, the interactions of MCD with CoA derivatives are not well understood owing to unavailable crystal structure with a complete occupancy in the active site. To identify the active site of MCD, molecular docking and molecular dynamics simulations were performed to explore the interactions of human mitochondrial MCD (HmMCD) and CoA derivatives. The findings reveal that the active site of HmMCD indeed resides in the prominent groove which resembles that of CurA. However, the binding modes are slightly different from the one observed in CurA due to the occupancy of the side chain of Lys183 from the N‐terminal helical domain instead of the adenine ring of CoA. The residues 300 ? 305 play an essential role in maintaining the stability of complex mainly through hydrogen bond interactions with the pyrophosphate moiety of acetyl‐CoA. Principle component analysis elucidates the conformational distribution and dominant concerted motions of HmMCD. MM_PBSA calculations present the crucial residues and the major driving force responsible for the binding of acetyl‐CoA. These results provide useful information for understanding the interactions of HmMCD with CoA derivatives. Proteins 2016; 84:792–802. © 2016 Wiley Periodicals, Inc. 相似文献
102.
103.
以稀土(Re~(3+))和落叶松单宁(LT)为原料,采用液相合成法合成了5种廉价的稀土-落叶松单宁(Re~(3+)-LT)配合物,并通过红外光谱、X射线光电子能谱、紫外光谱以及配位数测定确定了配合物的结构.采用牛津杯法、琼脂稀释法测定配合物对黑曲霉、红曲霉、白腐菌、毛霉4种真菌的抑制作用.在抑菌方面,5种配合物对上述4种真菌均具有较强的抑制作用,其抑菌活性大小顺序为Ce~(3+)-LTGd~(3+)-LTLa~(3+)-LTNd~(3+)-LTYb~(3+)-LT,其中Ce~(3+)-LT对4种真菌的最小抑菌浓度分别为:1.6、1.6、0.8和1.6 g·L~(-1);Yb~(3+)-LT对4种真菌的最小抑菌浓度分别为:3.2、1.6、3.2和3.2 g·L~(-1).在杀菌方面,Yb~(3+)-LT的杀菌活性最强,其对4种真菌的最小杀菌浓度分别为:6.4、3.2、3.2和6.4 g·L~(-1).此外,尽管Nd~(3+)-LT和Gd~(3+)-LT具有较强的抑菌活性,但对黑曲霉和毛霉的杀菌作用较弱. 相似文献
104.
L. Zheng Z. Y. Peng Q. Q. Jiao Y. Wang F. Bian S. J. Qu S. B. Wan Y. P. Bi 《Russian Journal of Plant Physiology》2016,63(5):673-677
The demand for INSULIN is increasing rapidly along with the increased number of diabetic patients. Using the CRE/loxP system, we developed a selective marker-free system without crossing to produce PROINSULIN in transgenic plant. In frame of this approach, the induced promoter pRD29A was isolated from Arabidopsis. The CRE recombinase gene was placed under the control of pRD29A between two loxP recombination sites together with the selective NPTII gene. Furthermore, the binary vector with CRE recombinase and PROINSULIN was constructed and introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. Gene excision was used to remove the sequence between the two loxP sites at the presence of 200 mM NaCl. PCR analysis showed that self-excision occurred in several T0 transgenic plants. Transgenic plants without any marker gene successfully expressed PROINSULIN. This auto-excision strategy provides efficient means of removing the selectable marker gene from transgenic plants. It is an efficient method for producing bio-safe recombinant protein and other valuable substances in plants. 相似文献
105.
Lin‐Li Lv Ye Feng Tao‐Tao Tang Bi‐Cheng Liu 《Journal of cellular and molecular medicine》2019,23(2):731-739
Extracellular vesicles (EVs) are released to maintain cellular homeostasis as well as to mediate cell communication by spreading protective or injury signals to neighbour or remote cells. In kidney, increasing evidence support that EVs are signalling vesicles for different segments of tubules, intra‐glomerular, glomerular‐tubule and tubule‐interstitial communication. EVs released by kidney resident and infiltrating cells can be isolated from urine and were found to be promising biomarkers for kidney disease, reflecting deterioration of renal function and histological change. We have here summarized the recent progress about the functional role of EVs in kidney disease as well as challenges and future directions involved. 相似文献
106.
Chong Chen Haining Tan Jiaqi Bi Lin Li Tianhua Rong Youxi Lin Peiyu Sun Jinqian Liang Yang Jiao Zheng Li Liang Sun Jianxiong Shen 《Journal of cellular and molecular medicine》2019,23(7):4582-4591
Congenital scoliosis (CS) is the result of anomalous vertebrae development, but the pathogenesis of CS remains unclear. Long non‐coding RNAs (lncRNAs) have been implicated in embryo development, but their role in CS remains unknown. In this study, we investigated the role and mechanisms of a specific lncRNA, SULT1C2A, in somitogenesis in a rat model of vitamin A deficiency (VAD)‐induced CS. Bioinformatics analysis and quantitative real‐time PCR (qRT‐PCR) indicated that SULT1C2A expression was down‐regulated in VAD group, accompanied by increased expression of rno‐miR‐466c‐5p but decreased expression of Foxo4 and somitogenesis‐related genes such as Pax1, Nkx3‐2 and Sox9 on gestational day (GD) 9. Luciferase reporter and small interfering RNA (siRNA) assays showed that SULT1C2A functioned as a competing endogenous RNA to inhibit rno‐miR‐466c‐5p expression by direct binding, and rno‐miR‐466c‐5p inhibited Foxo4 expression by binding to its 3′ untranslated region (UTR). The spatiotemporal expression of SULT1C2A, rno‐miR‐466c‐5p and Foxo4 axis was dynamically altered on GDs 3, 8, 11, 15 and 21 as detected by qRT‐PCR and northern blot analyses, with parallel changes in Protein kinase B (AKT) phosphorylation and PI3K expression. Taken together, our findings indicate that SULT1C2A enhanced Foxo4 expression by negatively modulating rno‐miR‐466c‐5p expression via the PI3K‐ATK signalling pathway in the rat model of VAD‐CS. Thus, SULT1C2A may be a potential target for treating CS. 相似文献
107.
Chronic myeloid leukemia (CML) is a lethal malignancy, and the progress toward long‐term survival has stagnated in recent decades. Pristimerin, a quinone methide triterpenoid isolated from the Celastraceae and Hippocrateaceae families, is well‐known to exert potential anticancer activities. In this study, we investigated the effects and the mechanisms of action on CML. We found that pristimerin inhibited cell proliferation of K562 CML cells by causing G1 phase arrest. Furthermore, we demonstrated that pristimerin triggered autophagy and apoptosis. Intriguingly, pristimerin‐induced cell death was restored by an autophagy inhibitor, suggesting that autophagy is cross‐linked with pristimerin‐induced apoptosis. Further studies revealed that pristimerin could produce excessive reactive oxygen species (ROS), which then induce JNK activation. These findings provide clear evidence that pristimerin might be clinical benefit to patients with CML. 相似文献
108.
Kang Shen Zeng Wang Xuanxuan Bi Yao Ying Duo Zhang Chengbin Jin Guangya Hou Huazhen Cao Liankui Wu Guoqu Zheng Yiping Tang Xinyong Tao Jun Lu 《Liver Transplantation》2019,9(20)
Lithium metal is the most attractive anode material due to its extremely high specific capacity, minimum potential, and low density. However, uncontrollable growth of lithium dendrite results in severe safety and cycling stability concerns, which hinders the application in next generation secondary batteries. In this paper, a new and facile method imposing a magnetic field to lithium metal anodes is proposed. That is, the lithium ions suffering Lorentz force due to the electromagnetic fields are put into spiral motion causing magnetohydrodynamics (MHD) effect. This MHD effect can effectively promote mass transfer and uniform distribution of lithium ions to suppress the dendrite growth as well as obtain uniform and compact lithium deposition. The results show that the lithium metal electrodes within the magnetic field exhibit excellent cycling and rate performance in a symmetrical battery. Additionally, full batteries using limited lithium metal as anodes and commercial LiFePO4 as cathodes show improved performance within the magnetic field. In summary, a new and facile strategy of suppressing lithium dendrites using the MHD effect by imposing a magnetic field is proposed, which may be generalized to other advanced alkali metal batteries. 相似文献
109.
110.