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991.
992.
Quinol/nitrate oxidoreductase (NarGHI) is the first enzyme involved in respiratory denitrification in prokaryotes. Although this complex in E. coli is known to operate with both ubi and menaquinones, the location and the number of quinol binding sites remain elusive. NarGHI strongly stabilizes a semiquinone radical located within the dihemic anchor subunit NarI. To identify its location and function, we used a combination of mutagenesis, kinetics, EPR, and ENDOR spectroscopies. For the NarGHIH66Y and NarGHIH187Y mutants lacking the distal heme bD, no EPR signal of the semiquinone was observed. In contrast, a semiquinone was detected in the NarGHIH56Y mutant lacking the proximal heme bP. Its thermodynamic properties and spectroscopic characteristics, as revealed by Q-band EPR and ENDOR spectroscopies, are identical to those observed in the native enzyme. The substitution by Ala of the Lys86 residue close to heme bD, which was previously proposed to be in a quinol oxidation site of NarGHI (QD), also leads to the loss of the EPR signal of the semiquinone, although both hemes are present. Enzymatic assays carried out on the NarGHIK86A mutant reveal that the substitution dramatically reduces the rate of oxidation of both mena and ubiquinol analogues. These observations demonstrate that the semiquinone observed in NarI is strongly associated with heme bD and that Lys86 is required for its stabilization. Overall, our results indicate that the semiquinone is located within the quinol oxidation site QD. Details of the possible binding motif of the semiquinone and mechanistic implications are discussed. 相似文献
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Rouyer Tristan Bernard Serge Kerzerho Vincent Giordano Nicolas Giordano François Ellul Salvu Ellul Giovanni Derridj Olivier Canet Rémy Deguara Simeon Wendling Bertrand Bonhommeau Sylvain 《Environmental Biology of Fishes》2022,105(5):635-644
Environmental Biology of Fishes - The purse seine fishery in the Mediterranean represents about 60% of the international catch for Atlantic Bluefin Tuna (Thunnus thynnus). Yet, tagging operations... 相似文献
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Cécile Boland Sophie Bertrand Wesley Mattheus Katelijne Dierick Vicky Jasson Toon Rosseel Steven Van Borm Jacques Mahillon Pierre Wattiau 《Applied and environmental microbiology》2015,81(9):3169-3175
Fifty-nine monophasic Salmonella enterica serovar Typhimurium isolates, collected in Belgium during the period from 2008 to 2011, have been serotyped as 4,[5]:i:− and shown to harbor an fljB coding sequence. The genetic differences between these strains and phenotypically biphasic Salmonella Typhimurium were analyzed through PCR and DNA sequencing. Genetic alterations in the fljB promoter region affecting expression of the phase 2 flagellin were observed in 53 isolates. Other genetic events in the invertible region carrying the fljB promoter were observed in 2 isolates. For the remaining 4 isolates, no molecular differences with a reference biphasic Salmonella Typhimurium strain could be observed. Next-generation sequencing of one representative isolate affected in the fljB promoter region revealed a 26-kb IS26 composite transposon insertion along with a local genomic rearrangement. Several other IS26 element-mediated alterations of this genomic region were observed. This group of monophasic Salmonella Typhimurium isolates was genetically heterogeneous, as revealed by multilocus variable-number tandem-repeat analysis (MLVA), PCR, and sequencing. Pigs and pork represented a major source of such monophasic isolates in Belgium, as reported in other countries. Three out of 5 isolates of human origin presented genetic profiles identical to those of food isolates, demonstrating the pathogenic potential of the newly characterized variants and potential dissemination along the food chain. This study highlighted the key role played by IS26 insertions in the loss of phase 2 flagellin expression and the subsequent generation of multiple monophasic variant lineages from biphasic Salmonella Typhimurium ancestors. 相似文献
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Morphological and plumage colour variation in the Réunion grey white‐eye (Aves: Zosterops borbonicus): assessing the role of selection 下载免费PDF全文
Joris A. M. Bertrand Yann X. C. Bourgeois Borja Milá Philipp Heeb Christophe Thébaud 《Biological journal of the Linnean Society. Linnean Society of London》2015,114(2):459-473
The Réunion grey white‐eye (Zosterops borbonicus), a small passerine endemic to the island of Réunion (Mascarene archipelago), constitutes an extraordinary case of phenotypic variation within a bird species, with conspicuous plumage colour differentiation at a microgeographical scale. To understand whether natural selection could explain such variability, we compared patterns of variation in morphological and plumage colour traits within and among populations. To quantify morphological variation, we used measurements obtained by Frank Gill in the 1960s from 239 individuals collected in 60 localities distributed over the entire island of Réunion. To quantify colour variation, we measured the reflectance spectra of plumage patches of 50 males from a subset of Gill's specimens belonging to the five recognized plumage colour variants and used a visual model to project these colours in an avian‐appropriate, tetrachromatic, colour space. We found that variants occupy different regions of the avian colour space and that between‐variant differences for most plumage patches could be discriminated by the birds. Differences in morphology were also detected, but these were, in general, smaller than colour differences. Overall, we found that variation in both plumage colour and morphology among variants is greater than would be expected if genetic drift alone was responsible for phenotypic divergence. As the plumage colour variants correspond to four geographical forms, our results suggest that phenotypic evolution in the Réunion grey white‐eye is at least partly explained by divergent selection in different habitats or regions. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2015, 114 , 459–473. 相似文献
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J A M Bertrand Y X C Bourgeois B Delahaie T Duval R García-Jiménez J Cornuault P Heeb B Milá B Pujol C Thébaud 《Heredity》2014,112(2):190-196
The Réunion grey white-eye, Zosterops borbonicus, a passerine bird
endemic to Réunion Island in the Mascarene archipelago, represents an extreme case
of microgeographical plumage colour variation in birds, with four distinct colour forms
occupying different parts of this small island (2512 km2). To understand
whether such population differentiation may reflect low levels of dispersal and gene flow
at a very small spatial scale, we examined population structure and gene flow by analysing
variation at 11 microsatellite loci among four geographically close localities
(<26 km apart) sampled within the distribution range of one of the colour forms,
the brown-headed brown form. Our results revealed levels of genetic differentiation that
are exceptionally high for birds at such a small spatial scale. This strong population
structure appears to reflect low levels of historical and contemporary gene flow among
populations, unless very close geographically (<10 km). Thus, we suggest that
the Réunion grey white-eye shows an extremely reduced propensity to disperse, which
is likely to be related to behavioural processes. 相似文献