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Intracellular compartmentation of inositol was demonstrated in primary cultures of mouse astrocytes, incubated in isotonic medium, by determination of efflux kinetics after loading with [3H]inositol. Three kinetically different compartments were delineated. The largest and most slowly exchanging compartment had a halflife of 9 hr. This slow release leads to retention of a sizeable amount of pre-accumulated inositol in the tissue 24 hr after the onset of uptake inhibition, as confirmed by the observation that the inositol uptake inhibitor fucose caused a larger inhibition of unidirectional inositol uptake than of inositol pool size, measured as accumulated [3H]inositol after 24 hr of combined exposure to the inhibitor and the labeled isotope. Based upon the present observations and literature data, it is suggested that the large, slowly exchanging compartment is largely membrane-associated and participating in signaling via the phosphatidylinositide second messenger system, whereas inositol functioning as an osmolyte is distributed in the cytosol and located in one or both of the compartments showing a faster release. 相似文献
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Elena Ian Dmitry B. Malko Olga N. Sekurova Harald Bredholt Christian Rückert Marina E. Borisova Andreas Albersmeier J?rn Kalinowski Mikhail S. Gelfand Sergey B. Zotchev 《PloS one》2014,9(5)
A total of 74 actinomycete isolates were cultivated from two marine sponges, Geodia barretti and Phakellia ventilabrum collected at the same spot at the bottom of the Trondheim fjord (Norway). Phylogenetic analyses of sponge-associated actinomycetes based on the 16S rRNA gene sequences demonstrated the presence of species belonging to the genera Streptomyces, Nocardiopsis, Rhodococcus, Pseudonocardia and Micromonospora. Most isolates required sea water for growth, suggesting them being adapted to the marine environment. Phylogenetic analysis of Streptomyces spp. revealed two isolates that originated from different sponges and had 99.7% identity in their 16S rRNA gene sequences, indicating that they represent very closely related strains. Sequencing, annotation, and analyses of the genomes of these Streptomyces isolates demonstrated that they are sister organisms closely related to terrestrial Streptomyces albus J1074. Unlike S. albus J1074, the two sponge streptomycetes grew and differentiated faster on the medium containing sea water. Comparative genomics revealed several genes presumably responsible for partial marine adaptation of these isolates. Genome mining targeted to secondary metabolite biosynthesis gene clusters identified several of those, which were not present in S. albus J1074, and likely to have been retained from a common ancestor, or acquired from other actinomycetes. Certain genes and gene clusters were shown to be differentially acquired or lost, supporting the hypothesis of divergent evolution of the two Streptomyces species in different sponge hosts. 相似文献
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We have used a clinical magnetic resonance imager to search for the possible effects of a 1.5 T magnetic fields on the growth of the yeast Saccharomyces cerevisiae. Yeast samples were grown in nutrient broth contained in constant-temperature boxes, both in and out of the magnetic field of the imager. Growth was measured by using a hemocytometer and light microscope to calculate cell densities. Over the time span corresponding to approximately seven cell divisions, we find no convincing statistical evidence for an effect of magnetic field on cell density. © 1994 Wiley-Liss, Inc. 相似文献
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Rausch Manuel K. Sugerman Gabriella P. Kakaletsis Sotirios Dortdivanlioglu Berkin 《Biomechanics and modeling in mechanobiology》2021,20(5):1645-1657
Biomechanics and Modeling in Mechanobiology - Blood clots play a diametric role in our bodies as they are both vital as a wound sealant, as well as the source for many devastating diseases. In... 相似文献