排序方式: 共有140条查询结果,搜索用时 15 毫秒
131.
Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system 总被引:1,自引:0,他引:1
Athina Avlami G. Ganteris Eleni Malamou-Lada Olga Paniara C.C. Papagiannitsis Ioanna Stefanou Helen Vagiakou 《Journal of microbiological methods》2010,83(2):185-187
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting blaVIM genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility. 相似文献
132.
Dimitra Vasilaki Athina Bakopoulou Alexandros Tsouknidas Elaine Johnstone Konstantinos Michalakis 《Biophysical reviews》2021,13(3):339
During metastasis, tumor cells need to adapt to their dynamic microenvironment and modify their mechanical properties in response to both chemical and mechanical stimulation. Physical interactions occur between cancer cells and the surrounding matrix including cell movements and cell shape alterations through the process of mechanotransduction. The latter describes the translation of external mechanical cues into intracellular biochemical signaling. Reorganization of both the cytoskeleton and the extracellular matrix (ECM) plays a critical role in these spreading steps. Migrating tumor cells show increased motility in order to cross the tumor microenvironment, migrate through ECM and reach the bloodstream to the metastatic site. There are specific factors affecting these processes, as well as the survival of circulating tumor cells (CTC) in the blood flow until they finally invade the secondary tissue to form metastasis. This review aims to study the mechanisms of metastasis from a biomechanical perspective and investigate cell migration, with a focus on the alterations in the cytoskeleton through this journey and the effect of biologic fluids on metastasis. Understanding of the biophysical mechanisms that promote tumor metastasis may contribute successful therapeutic approaches in the fight against cancer. 相似文献
133.
Sereti Vasileia Lazaridou Athina Tananaki Chrysoula Biliaderis Costas G. 《Food biophysics》2021,16(3):365-380
Food Biophysics - Honey from cotton blossoms tends to crystallize rapidly resulting in a product with hard texture and low spreadability, even if it is processed via a controlled crystallization... 相似文献
134.
Athina Economou-Amilli 《Hydrobiologia》1980,74(1):39-48
Based on a microfloristic analysis of periphyton, a selected ecological method of evaluating water quality, independent of saprobic systems is applied to a chosen section of running water in the springs of Levadia, Greece. The graphic representation of the results and an estimation of the degree of pollution is given. A simple method for the quantitative evaluation of the biocoenose of epilithic algae is additionally developed. 相似文献
135.
Mentha spicata and Salvia fruticosa composts as soil amendments in tomato cultivation 总被引:1,自引:0,他引:1
Dimitris Chalkos Kalliopi Kadoglidou Katerina Karamanoli Christina Fotiou Athina S. Pavlatou-Ve Ilias G. Eleftherohorinos Helen-Isis A. Constantinidou Despoina Vokou 《Plant and Soil》2010,332(1-2):495-509
Τhe potential use of the aromatic plants Mentha spicata L. (spearmint) and Salvia fruticosa Mill. (sage) as soil amendments was evaluated. For this purpose, tomato seeds were sown in pots that had been filled with composts made from these plants and mixed with soil collected from an organically cultivated tomato field. A 2?×?2?×?4 [two types of fertilizer (synthetic and organic), two types of compost (M. spicata and S. fruticosa) and four compost rates (0%, 2%, 4% and 8%, w/w)] factorial experiment was used; the experiment was conducted twice in a growth chamber and lasted 60 days. At 0, 20, 40 and 60 days, after the establishment of the experiment, the soil bacterial and fungal abundance, the growth of nitrifying bacteria, the number of emerging weeds and the shoot length of tomato plants were measured in all treatments; at the end of the experiment, the above and belowground biomass of tomato plants was also determined. Soil microbial density increased with increasing compost rate of both species; the highest fungal and bacterial densities were recorded at 40 and 60 days, after the establishment of the experiment, respectively. Nitrifying bacteria were present in all treatments and at all sampling times. Both composts had a stimulating effect on tomato growth, which was remarkably pronounced with M. spicata. In contrast, weed emergence was reduced, but only in soils amended with M. spicata. The results suggest that M. spicata compost added at a rate of 4% to 8% is a very promising soil amendment, since it stimulates tomato growth, increases soil bacterial and fungal abundance and inhibits weed emergence. Further research is needed to elucidate the mode of action of M. spicata compost, its effect under field conditions and its possible use in mixed crop, rotational crop or cover crop systems. 相似文献
136.
Anaïs Llorens Athina Tzovara Ludovic Bellier Ilina Bhaya-Grossman Aurélie Bidet-Caulet William K. Chang Zachariah R. Cross Rosa Dominguez-Faus Adeen Flinker Yvonne Fonken Mark A. Gorenstein Chris Holdgraf Colin W. Hoy Maria V. Ivanova Richard T. Jimenez Soyeon Jun Julia W.Y. Kam Celeste Kidd Nina F. Dronkers 《Neuron》2021,109(13):2047-2074
137.
John Ong Junzhe Zhao Alexander W. Justin Athina E. Markaki 《Biotechnology and bioengineering》2019,116(12):3457-3468
Albumin, the most abundant plasma protein in mammals, is a versatile and easily obtainable biomaterial. It is pH and temperature responsive, dissolvable in high concentrations and gels readily in defined conditions. This versatility, together with its inexpensiveness and biocompatibility, makes albumin an attractive biomaterial for biomedical research and therapeutics. So far, clinical research in albumin has centered mainly on its use as a carrier molecule or nanoparticle to improve drug pharmacokinetics and delivery to target sites. In contrast, research in albumin-based hydrogels is less established albeit growing in interest over recent years. In this minireview, we report current literature and critically discuss the synthesis, mechanical properties, biological effects and uses, biodegradability and cost of albumin hydrogels as a xeno-free, customizable, and transplantable construct for tissue engineering and regenerative medicine. 相似文献
138.
139.
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine affecting diverse cellular responses. TNF-α is cytotoxic in many systems, but it can also act as an anti-apoptotic signal to promote cell survival pathways activated through integrins and extracellular matrix components. This is particularly evident in cancer cells. To unravel the basis of resistance to TNF-α-induced apoptosis, human osteosarcoma MG-63 cell line was used. Our data showed that resistance to apoptosis was accompanied by high levels of TIMP-1 expression in part mediated by NF-κB activation, whereas under apoptotic conditions, in the presence of cycloheximide (CHX), TIMP-1 and αvβ3 integrin protein levels were significantly reduced. Silencing TIMP-1 using siRNA led to increased apoptosis following treatment with TNF-α, whereas exogenously-added recombinant TIMP-1 reduced the extent of apoptosis. Immunoprecipitation and confocal microscopy experiments demonstrated that TIMP-1 interacted with αvβ3 integrins. The biological role of this interaction was revealed by the use of echistatin, an antagonist of αvβ3 integrin. In the presence of echistatin, decreased protection against apoptosis by recombinant TIMP-1 was observed. 相似文献
140.
Ruchira Chatterjee Louise Lassalle Sheraz Gul Franklin D. Fuller Iris D. Young Mohamed Ibrahim Casper de Lichtenberg Mun Hon Cheah Athina Zouni Johannes Messinger Vittal K. Yachandra Jan Kern Junko Yano 《Physiologia plantarum》2019,166(1):60-72
In nature, an oxo‐bridged Mn4CaO5 cluster embedded in photosystem II (PSII), a membrane‐bound multi‐subunit pigment protein complex, catalyzes the water oxidation reaction that is driven by light‐induced charge separations in the reaction center of PSII. The Mn4CaO5 cluster accumulates four oxidizing equivalents to enable the four‐electron four‐proton catalysis of two water molecules to one dioxygen molecule and cycles through five intermediate S‐states, S0 – S4 in the Kok cycle. One important question related to the catalytic mechanism of the oxygen‐evolving complex (OEC) that remains is, whether structural isomers are present in some of the intermediate S‐states and if such equilibria are essential for the mechanism of the O‐O bond formation. Here we compare results from electron paramagnetic resonance (EPR) and X‐ray absorption spectroscopy (XAS) obtained at cryogenic temperatures for the S2 state of PSII with structural data collected of the S1, S2 and S3 states by serial crystallography at neutral pH (~6.5) using an X‐ray free electron laser at room temperature. While the cryogenic data show the presence of at least two structural forms of the S2 state, the room temperature crystallography data can be well‐described by just one S2 structure. We discuss the deviating results and outline experimental strategies for clarifying this mechanistically important question. 相似文献