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Antibodies to tubulin were prepared in rabbits by immunization with reduced-carboxymethylated calf-brain tubulin. In immunodiffusion tests the antibodies showed full cross reactivity with the immunogen as well as with native calf-brain tubulin. The same antibodies showed cross reactivity with a factor in extract of cotton (Gossypium hirsutum L.) cotyledons but there was no full immunological identity between calf-brain tubulin and this factor. A solid-phase radioimmunoassay for quantitative estimation of this plant tubulin-like factor was developed. It measured the binding of antibodies to immobilized calf-native tubulin. Competition between the unknown soluble tubulin-like factor, and immobilized tubulin was assayed at serum dilution of 1:50. Extraction conditions which preserved the antigenic properties of the tubulin-like factor from cotton cotyledons were defined. The radioimmunoassay measured quantities of the tubulin-like factor in the range of 0.1–10 g-equivalents of calf-brain tubulin. Immediately after homogenization of the tissue only 25% of the total amount of tubulin-like activity was present in soluble form, while most of it remained in the insoluble fraction. Apparent maximal solubilization was achieved spontaneously 10 h after homogenization or by treatment with guanidine hydrochloride. These results indicate that in this material, tubulin is not released immediately by homogenization but remains assembled in microtubules and-or in a bound or sequestered form.Abbreviations NRS
normal rabbit serum
- RCM
reduced-carboxymethylated 相似文献
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Chilling Injury in Cotton {Gossypium hirsutum L.): Light Requirement for the Reduction of Injury and for the Protective Effect of Abscisic Acid 总被引:1,自引:0,他引:1
Pretreatment by darkness increased chilling (4°C) injuryin whole cotton (Gossypium hirsutum L.) seedlings and isolatedcotyledonary tissue. Addition of sucrose in the dark periodprevented the effect of darkness. Application of the photosyntheticinhibitor DCMU in light simulated the effect of darkness. ABA(105 M) decreased chilling injury when applied in lightas a pretreatment before the onset of chilling. The same pretreatmentin darkness was almost ineffective, unless sucrose was added.ABA applied in light together with DCMU was ineffective in decreasingchilling injury. Lower light intensity resulted in increasedchilling injury and a decreased effect of ABA in the preventionof chilling injury. The antimicrotubular drug colchicine increased the chillinginjury. Pretreatment with ABA in light decreased the chillingand colchicine injury while the same pretreatment in darknesswas ineffective. These results suggest that a deficiency of a photosyntheticproduct increases the chilling sensitivity of the tissue. ABAapparently increases chilling resistance through a metabolicprocess which depends on photosynthetic activity.
3 Incumbent of the Seagram Chair in Plant Sciences (Received November 20, 1980; Accepted January 31, 1981) 相似文献
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The theory of computational complexity and certain explicitly-stated hypotheses imply limitations on the information processing
power of biological systems. Parallelism, special purpose organization, and analog mechanisms may provide speedup critical
for life processes, but have little power in the face of exponential growth. We show that “polynomially simulatable” biological
systems cannot exhibit dynamic behavior which produces the solution of an intractable problem. The argument implies that parallelism
does not allow biological systems to defeat the exponential explosion, but rather is important because it allows polynomial
time algorithms to be used more efficiently. 相似文献
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The genetic control of susceptibility to experimental allergic encephalomyelitis (EAE) was studied in mice. The results indicate that sensitivity to disease is not inherited in a simple Mendelian dominant way. Susceptibility to EAE is governed by genes located outside of the major histocompatibility complex and not byH-2-linkedIr genes. No correlation was observed between susceptibility to disease and the cellular immune response toward the small encephalitogenic protein. 相似文献
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The effect of NADP+ on light-induced steady-state redox changes of membrane-bound cytochromes was investigated in membrane fragments prepared from the blue-green algae Nostoc muscorum (Strain 7119) that had high rates of electron transport from water to NADP+ and from an artificial electron donor, reduced dichlorophenolindophenol (DCIPH2) to NADP+. The membrane fragments contained very little phycocyanin and had excellent optical properties for spectrophotometric assays. With DCIPH2 as the electron donor, NADP+ had no effect on the light-induced redox changes of cytochromes: with or without NADP+, 715- or 664-nm illumination resulted mainly in the oxidation of cytochrome f and of other component(s) which may include a c-type cytochrome with an α peak at 549 nm. With 664 nm illumination and water as the electron donor, NADP+ had a pronounced effect on the redox state of cytochromes, causing a shift toward oxidation of a component with a peak at 549 nm (possibly a c-type cytochrome), cytochrome f, and particularly cytochrome b559. Cytochrome b559 appeared to be a component of the main noncyclic electron transport chain and was photooxidized at physiological temperatures by Photosystem II. This photooxidation was apparent only in the presence of a terminal acceptor (NADP+) for the electron flow from water. 相似文献
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Samuel H. Light Sankar N. Krishna Raymond C. Bergan Arnon Lavie Wayne F. Anderson 《Journal of structural and functional genomics》2013,14(1):25-30
Dehydroquinate dehydratase (DHQD) catalyzes the third step in the biosynthetic shikimate pathway. Here we identify a Bifidobacterium longum protein with high sequence homology to type II DHQDs but no detectable DHQD activity under standard assay conditions. A crystal structure reveals that the B. longum protein adopts a DHQD-like tertiary structure but a distinct quaternary state. Apparently forming a dimer, the B. longum protein lacks the active site aspartic acid contributed from a neighboring protomer in the type II DHQD dodecamer. Relating to the absence of protein–protein interactions established in the type II DHQD dodecameric assembly, substantial conformational changes distinguish the would-be active site of the B. longum protein. As B. longum possess no other genes with homology to known DHQDs, these findings imply a unique DHQD activity within B. longum. 相似文献
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Acetylcholinesterase of Schistosoma mansoni--functional correlates. Contributed in honor of Professor Hans Neurath's 90th birthday 下载免费PDF全文
Arnon R Silman I Tarrab-Hazdai R 《Protein science : a publication of the Protein Society》1999,8(12):2553-2561
Acetylcholinesterase (AChE) is an enzyme broadly distributed in many species, including parasites. It occurs in multiple molecular forms that differ in their quaternary structure and mode of anchoring to the cell surface. This review summarizes biochemical and immunological investigations carried out in our laboratories on AChE of the helmint, Schistosoma mansoni. AChE appears in S. mansoni in two principal molecular forms, both globular, with sedimentation coefficients of approximately 6.5 and 8 S. On the basis of their substrate specificity and sensitivity to inhibitors, both are "true" acetylcholinesterases. Approximately half of the AChE activity of S. mansoni is located on the outer surface of the parasite, attached to the tegumental membrane via a covalently attached glycosylphosphatidylinositol anchor. The remainder is located within the parasite, mainly associated with muscle tissue. Whereas the internal enzyme is most likely involved in termination of neurotransmission at cholinergic synapses, the role of the surface enzyme remains to be established; there are, however, indications that it is involved in signal transduction. The two forms of AChE differ in their heparin-binding properties, only the internal 8 S form of the AChE being retained on a heparin column. The two forms differ also in their immunological specificity, since they are selectively recognized by different monoclonal antibodies. Polyclonal antibodies raised against S. mansoni AChE purified by affinity chromatography are specific for the parasite AChE, reacting with both molecular forms, but do not recognize AChE from other species. They interact with the surface-localized enzyme on the intact organism, and produce almost total complement-dependent killing of the parasite. S. mansoni AChE is thus demonstrated to be a functional protein, involved in multifaceted activities, which can serve as a suitable candidate for diagnostic purposes, vaccine development, and drug design. 相似文献
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