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11.
Organization and nucleotide sequence of a gene cluster coding for eight ribosomal proteins in the archaebacterium Halobacterium marismortui 总被引:7,自引:0,他引:7
A DNA fragment containing the genes for the eight ribosomal proteins HmaL3, HL6, HmaL23, HmaL2, HmaS19, HmaL22, HmaS3, and HmaL29 from Halobacterium marismortui has been cloned and sequenced. The organization of this gene cluster in general corresponds to the S10 operon of Escherichia coli although there exists some differences between them. The sequence analysis of the 5'- and 3'-region of the gene cluster revealed three open reading frames (orf1, orf2, and orf3) which do not code for any ribosomal protein whose structure is known. A putative promoter is located upstream of orf1. Out of the eight ribosomal proteins five have counterparts in eubacteria only, two in both eubacteria and eukaryotes, and one is exclusively related to an eukaryotic ribosomal protein. 相似文献
12.
Prior to anticipated nasal surgery, the nasal and facial skin should be examined for any vascular lesions. The skin type should be ascertained. A history of any prior nasal surgery, particularly on the nasal dorsum, should be noted. If rosacea is a clinical possibility, a trial of 1.5 to 2.0 gm q.d. of tetracycline for 6 to 8 weeks is warranted. If, after rhinoplasty, a diffuse "redness" on the nasal dorsum results and one can exclude other diagnoses, then argon laser therapy should be considered. A 3-mm punch biopsy should be obtained to see whether superficial ectatic vessels are present, a finding that would be indicative of a good result from laser therapy. 相似文献
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Synopsis The aim of this paper is the selective visualization of human blood basophils in autoradiographs. [3H]Thymidine-labelled basophils in buffy coat smears were fixed in methanol-formaldehyde followed by 5-aminoacridine hydrochloride and stained with basic Aldehyde Fuchsin prior to autoradiographic processing. The technique described represents a simple method for quantitative autoradiographic studies on basophil kinetics and the interaction of these cells with IgE-mediated atopias. 相似文献
15.
Antigenic specificity of red and white muscle myosin. 总被引:10,自引:0,他引:10
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The effect of the thermal fluctuation on the orientation distribution pattern of globular protein molecules in a two-dimensional lattice was investigated by the method of computer simulation. A set of interaction parameters was assigned to interaction sites on each molecule and the interaction energy between two molecules was given by the product of the parameters of facing sites. Orientation fluctuation was assumed to take place with the probability proportional to the Boltzmann factor. Patterns having different degrees of order appeared with the change of temperature. The entropy and other thermodynamic quantities of these patterns were calculated, and gradual and transitional changes of the pattern were discussed in comparison with the case of simple atoms or molecules. 相似文献
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Fritz Joachim Sedlazeck Prabhavathi Talloji Arndt von Haeseler Andreas Bachmair 《Genomics》2013,101(3):204-209
Identification of single nucleotide polymorphisms (SNPs) is a key element in sequence-based genetic analysis. Next generation sequencing offers a cost-effective basis to generate the necessary, large sequence data sets, and bioinformatic methods are being developed to process sequencing machine readouts. We were interested in detection of SNPs in a 350 kb region of an EMS-mutagenized Arabidopsis chromosome 3. The region was selectively analyzed using PCR-generated, overlapping fragments for Solexa sequencing. The ensuing reads provided a high coverage and were processed bioinformatically. In order to assess the SNP candidates obtained with a frequently used alignment program and SNP caller, we developed an additional method that allows the identification of high confidence SNP loci. The method can easily be applied to complete genome sequence data of sufficient coverage. 相似文献
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Claire Farrell Christopher Szota Nicholas S. G. Williams Stefan K. Arndt 《Plant and Soil》2013,372(1-2):177-193