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71.
P J Duerksen-Hughes D B Day S M Laster N A Zachariades L Aquino L R Gooding 《Journal of immunology (Baltimore, Md. : 1950)》1992,149(6):2114-2122
SV40 transformation of rodent fibroblasts generally produces cells that are highly sensitive to killing by activated macrophages. The cell line SV-COL-E8 (E8) is typical of SV40-transformed mouse fibroblasts in that it is readily lysed when exposed to activated macrophages. This killing is not due solely to TNF, because soluble TNF alone is incapable of lysing these cells. TNF is, however, necessary for lysis since antibodies to TNF will prevent macrophage-mediated lysis. Similarly, E8 is not sensitive to nitric oxide (NO); however, NO is also necessary for lysis since inhibition of NO generation (by coincubation with the arginine analogue NG-monomethyl-1-arginine) with Fe(II)) blocks lysis of E8 by activated macrophages. Cytolysis by macrophages is contact dependent, suggesting that the cell-associated TNF precursor may be involved in mediating cytolysis. However, transfected cell lines bearing cell-associated TNF precursor do not mediate killing of E8. Thus, killing of E8 either involves both TNF and NO in addition to a third, as yet unidentified, lytic mechanism, or killing requires the contact-dependent delivery of TNF and NO from the macrophage to its target. 相似文献
72.
Marcos Fábio Gadelha Rocha Jamille Alencar Sales Maria Gleiciane da Rocha Livia Maria Galdino Lara de Aguiar Waldemiro de Aquino Pereira-Neto 《Biofouling》2019,35(3):320-328
This study aimed to determine the minimum inhibitory concentration (MIC) of kaempferol and quercetin against planktonic and biofilm forms of the Candida parapsilosis complex. Initially, nine C. parapsilosis sensu stricto, nine C. orthopsilosis and nine C. metapsilosis strains were used. Planktonic susceptibility to kaempferol and quercetin was assessed. Growing and mature biofilms were then exposed to the flavonoids at MIC or 10xMIC, respectively, and theywere also analyzed by confocal laser scanning microscopy. The MIC ranges were 32-128 µg ml?1 for kaempferol and 0.5-16 µg ml?1 for quercetin. Kaempferol and quercetin decreased (P?<?0.05) the metabolic activity and biomass of growing biofilms of the C. parapsilosis complex. As for mature biofilms, the metabolic effects of the flavonoids varied, according to the cryptic species, but kaempferol caused an overall reduction in biofilm biomass. Microscopic analyses showed restructuring of biofilms after flavonoid exposure. These results highlight the potential use of these compounds as sustainable resources for the control of fungal biofilms. 相似文献
73.
Growth,photosynthetic activity and tuber quality of two potato cultivars in controlled environment as affected by light source 总被引:1,自引:0,他引:1
Roberta Paradiso Carmen Arena Youssef Rouphael Luigi d’Aquino Konstantinos Makris Paola Vitaglione 《Plant biosystems》2019,153(5):725-735
In order to elucidate the effect of genetic material and light source and their interaction on plant performance of potato (Solanum tuberosum L.), we studied the influence of two light sources, white fluorescent tubes (WF) and red blue LEDs with ratio 8:1 (RB) and two cultivars, ‘Avanti’ and ‘Colomba’ grown in phytotron, on growth, leaf photosynthesis and photochemistry and tuber quality. Under WF, net photosynthesis (NP) increased from the vegetative phase until flowering, then decreased during tuber bulking, with no differences between the cultivars. Lighting with RB increased the NP and the PSII maximum quantum use efficiency compared to WF. RB reduced stem elongation in both cultivars, as well as the number and area of leaves, and the aerial biomass per plant in ‘Colomba’, compared to WF. Conversely, tuber yield was higher in plants under RB light in both ‘Avanti’ and ‘Colomba’. Light source did not influence the tuber content of starch and total glycoalkaloids, while it affected differently in the cultivars the protein content and the glycoalkaloid profile. Our results highlight how interactions between light source and genotype need to be considered for potato cultivation in controlled environment under artificial lighting. 相似文献
74.
First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil 下载免费PDF全文
Monally C. C. Aquino Giovanni Widmer Anaiza S. Zucatto Milena A. Viol Sandra V. Inácio Alex A. Nakamura Willian M. D. Coelho Silvia H. V. Perri Marcelo V. Meireles Katia D. S. Bresciani 《The Journal of eukaryotic microbiology》2015,62(5):657-661
With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes. 相似文献
75.
Dora JM Kelbert S Deutschendorf C Cunha VS Aquino VR Santos RP Goldani LZ 《Mycopathologia》2006,161(4):235-238
Cutaneous cryptococcosis caused by C. gattii, in immunocompent patients is a rare manifestation of disease, and may be one of the first manifestations of disseminated
cryptococcosis. We report a case of disseminated cryptococcosis caused by Cryptococcus gattii presenting as cutaneous lesions in an immunocompetent patient. Previously to our report, only five cases of cutaneous involvement
by Cryptococcus gattii in immunocompetent patients have been reported in the literature. Risk factors for C. gattii infection included exposure to the eucalypt reservoirs in tropical and subtropical areas. Skin involvement corresponded to
the disseminated form of cryptococcosis in the majority of patients, and commonly affected the face and neck with different
morphologies including papules, pustules, plaques, ulcers, subcutaneous masses, cellulitis or acneiform lesions. Due to the
severity of this infection and the life threatening condition that it represents, clinicians must be aware that cutaneous
involvement may be one of the first manifestations of disseminated cryptococcosis caused by C. gattii especially in patients living and coming from endemic areas. 相似文献
76.
77.
Yaqin Xu Christine Tertilt Anja Krause Luis EN Quadri Ronald G Crystal Stefan Worgall 《Respiratory research》2009,10(1):26
Background
Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Infections of the respiratory tract are a hallmark in CF. The host immune responses in CF are not adequate to eradicate pathogens, such as P. aeruginosa. Dendritic cells (DC) are crucial in initiation and regulation of immune responses. Changes in DC function could contribute to abnormal immune responses on multiple levels. The role of DC in CF lung disease remains unknown.Methods
This study investigated the expression of CFTR gene in bone marrow-derived DC. We compared the differentiation and maturation profile of DC from CF and wild type (WT) mice. We analyzed the gene expression levels in DC from naive CF and WT mice or following P. aeruginosa infection.Results
CFTR is expressed in DC with lower level compared to lung tissue. DC from CF mice showed a delayed in the early phase of differentiation. Gene expression analysis in DC generated from naive CF and WT mice revealed decreased expression of Caveolin-1 (Cav1), a membrane lipid raft protein, in the CF DC compared to WT DC. Consistently, protein and activity levels of the sterol regulatory element binding protein (SREBP), a negative regulator of Cav1 expression, were increased in CF DC. Following exposure to P. aeruginosa, expression of 3β-hydroxysterol-Δ7 reductase (Dhcr7) and stearoyl-CoA desaturase 2 (Scd2), two enzymes involved in the lipid metabolism that are also regulated by SREBP, was less decreased in the CF DC compared to WT DC.Conclusion
These results suggest that CFTR dysfunction in DC affects factors involved in membrane structure and lipid-metabolism, which may contribute to the abnormal inflammatory and immune response characteristic of CF. 相似文献78.
P.M. Drysdale J.P. Huber P.A. Robinson K.M. Aquino 《Journal of theoretical biology》2010,265(4):524-79
A quantitative theory is developed for the relationship between stimulus and the resulting blood oxygen level dependent (BOLD) functional magnetic resonance imaging (fMRI) signal, including both spatial and temporal dynamics for the first time. The brain tissue is modeled as a porous elastic medium, whose interconnected pores represent the vasculature. The model explicitly incorporates conservation of blood mass, interconversion of oxygenated and deoxygenated hemoglobin, force balance within the blood and of blood pressure with vessel walls, and blood flow modulation due to neuronal activity. In appropriate limits it is shown to reproduce prior Balloon models of hemodynamic response, which do not include spatial variations. The regime of validity of such models is thereby clarified by elucidating their assumptions, and when these break down, for example when voxel sizes become small. 相似文献
79.
Anh P. Truong Gary D. Probst Jose Aquino Larry Fang Louis Brogley Jennifer M. Sealy Roy K. Hom John A. Tucker Varghese John Jay S. Tung Michael A. Pleiss Andrei W. Konradi Hing L. Sham Michael S. Dappen Gergley Tóth Nanhua Yao Eric Brecht Hu Pan Dean R. Artis Lany Ruslim John-Michael Sauer 《Bioorganic & medicinal chemistry letters》2010,20(16):4789-4794
Herein we describe further evolution of hydroxyethylamine inhibitors of BACE-1 with enhanced permeability characteristics necessary for CNS penetration. Variation at the P2′ position of the inhibitor with more polar substituents led to compounds 19 and 32, which retained the potency of more lipophilic analog 1 but with much higher observed passive permeability in MDCK cellular assay. 相似文献
80.
Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R. Teixeira Jesus G. Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral 《PLoS neglected tropical diseases》2010,4(3)