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81.
Dhanashree?A?Paranjpe D?Anitha MK?Chandrashekaran Amitabh?Joshi Vijay?Kumar?SharmaEmail author 《BMC developmental biology》2005,5(1):5
Background
In insects, circadian clocks have been implicated in affecting life history traits such as pre-adult development time and adult lifespan. Studies on the period (per) mutants of Drosophila melanogaster, and laboratory-selected lines of Bactrocera cucurbitae suggested a close link between circadian clocks and development time. There is a possibility of clock genes having pleiotropic effects on clock period and pre-adult development time. In order to avoid such pleiotropic effects we have used wild type flies of same genotype under environments of different periodicities, which phenotypically either speeded up or slowed down the eclosion clock of D. melanogaster. 相似文献82.
Anitha?Ramaswamy E?Lin Iou?Chen Rahul?Mitra Joel?Morrisett Kevin?Coombes Zhenlin?Ju Mini?KapoorEmail author 《Proteome science》2005,3(1):9
This study presents the development and application of protein lysate microarray (LMA) technology for verification of presence
and quantification of human tissue samples for protein biomarkers. Sub-picogram range sensitivity has been achieved on LMA
using a non-enzymatic protein detection methodology. Results from a set of quality control experiments are presented and demonstrate
the high sensitivity and reproducibility of the LMA methodology. The optimized LMA methodology has been applied for verification
of the presence and quantification of disease markers for atherosclerosis. LMA were used to measure lipoprotein [a] and apolipoprotein
B100 in 52 carotid endarterectomy samples. The data generated by LMA were validated by ELISA using the same protein lysates.
The correlations of protein amounts estimated by LMA and ELISA were highly significant, with r2 ≥ 0.98 (p ≤ 0.001) for lipoprotein [a] and with r2 ≥ 0.94 (p ≤ 0.001) for apolipoprotein B100. This is the first report to compare data generated using proteins microarrays
with ELISA, a standard technology for the verification of the presence of protein biomarkers. The sensitivity, reproducibility,
and high-throughput quality of LMA technology make it a potentially powerful technology for profiling disease specific protein
markers in clinical samples. 相似文献
83.
Cost-effective and rapid multiplication of Nomuraea rileyi is reported. The spore yields in semi-synthetic media were comparable or significantly higher to the standard medium. Maltose and peptone, carbon and nitrogen sources could be effectively replaced with 2% barley extract and 1% soybean extract respectively. However, replacement of yeast extract with dry yeast resulted in lower spore yields. Sporulation of the fungus multiplied on solid substrate was possible only when the bags used had a 0.2 m filter to facilitate passive exchange of sterile air. A high spore yield of 2.8 × 109/g of substrate was realized on crushed sorghum.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
84.
Nagaraju GP Basha MR Reddy PS 《Zeitschrift für Naturforschung. C, Journal of biosciences》2001,56(3-4):315-317
Injection of three different organotin compounds such as tripalmitin, fentin and fenbutatin produced a significant increase in the hemolymph sugar level of intact crabs at Oziotelphusa senex senex apparently by stimulating release of the hyperglycemic hormone (HGH). 相似文献
85.
Christopher R. Heier Alfredo D. Guerron Alexandru Korotcov Stephen Lin Heather Gordish-Dressman Stanley Fricke Raymond W. Sze Eric P. Hoffman Paul Wang Kanneboyina Nagaraju 《PloS one》2014,9(11)
In Duchenne muscular dystrophy (DMD), a genetic disruption of dystrophin protein expression results in repeated muscle injury and chronic inflammation. Magnetic resonance imaging shows promise as a surrogate outcome measure in both DMD and rehabilitation medicine that is capable of predicting clinical benefit years in advance of functional outcome measures. The mdx mouse reproduces the dystrophin deficiency that causes DMD and is routinely used for preclinical drug testing. There is a need to develop sensitive, non-invasive outcome measures in the mdx model that can be readily translatable to human clinical trials. Here we report the use of magnetic resonance imaging and spectroscopy techniques for the non-invasive monitoring of muscle damage in mdx mice. Using these techniques, we studied dystrophic mdx muscle in mice from 6 to 12 weeks of age, examining both the peak disease phase and natural recovery phase of the mdx disease course. T2 and fat-suppressed imaging revealed significant levels of tissue with elevated signal intensity in mdx hindlimb muscles at all ages; spectroscopy revealed a significant deficiency of energy metabolites in 6-week-old mdx mice. As the mdx mice progressed from the peak disease stage to the recovery stage of disease, each of these phenotypes was either eliminated or reduced, and the cross-sectional area of the mdx muscle was significantly increased when compared to that of wild-type mice. Histology indicates that hyper-intense MRI foci correspond to areas of dystrophic lesions containing inflammation as well as regenerating, degenerating and hypertrophied myofibers. Statistical sample size calculations provide several robust measures with the ability to detect intervention effects using small numbers of animals. These data establish a framework for further imaging or preclinical studies, and they support the development of MRI as a sensitive, non-invasive outcome measure for muscular dystrophy. 相似文献
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The effect of alkyl substitution (CH3, C2H5, n-C3H7, i-C3H7, and t-C4H9) on the hydrogen bond strengths (H-bond) of substituted amide-alcohol complexes has been systematically explored. B3LYP/aug-cc-pVDZ
method was applied to a total of 215 alkyl substituted amide-alcohol complexes to delineate the effect of substitution on
the H-bond strength; formamide-water complex is taken as reference point. Complexes are classified into five types depending
on the hydrogen donor, acceptor and the site of alkyl substitution (Type-IA, Type-IIA, Type-IB, Type-IIB and Type-III). The
strength of H-bond was correlated with geometrical parameters such as proton-acceptor (H∙∙∙∙Y) distance, the length of proton
donating bond (X–H). In all the complexes N–H and O–H stretching frequencies are red-shifted. The effect of alkyl substitution
on N–H and O–H stretching frequencies were analyzed. Topological parameters like electron density at H∙∙∙∙Y and X–H bond critical
points as derived from atom in molecules (AIM) theory was also evaluated. When C = O group is participating in H-bond, the
strength of H-bond decreases with increasing size of alcohols except for methanol (Type-IA, Type-III and Type-IB complexes).
But it increases with increasing size of alkyl groups on amide and decreases with bulky groups. In the case of N–H group as
H-bond donor, the strength of H-bond increases with increasing size of alcohols (Type-IIA and Type-IIB complexes) whereas
decreases with increasing size of alkyl groups on amide. Type-IA, IIA, IB and IIB complexes exhibit good correlations among
IE, H-bond distance and electron density at bcp. In Type-III complexes, average H-bond distance and sum of electron densities
shows better correlation with IEs than the corresponding individuals. The correlation of IE less with electron density at
RCP compared to sum of electron densities. 相似文献
90.