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381.
Angelina García Darío A. Dermarchi Luciana Tovo-Rodrigues Maia Pauro Sidia M. Callegari-Jacques Francisco M. Salzano Mara H. Hutz 《Genetics and molecular biology》2015,38(3):324-331
The population of Argentina has already been studied with regard to several genetic markers, but much more data are needed for the appropriate definition of its genetic profile. This study aimed at investigating the admixture patterns and genetic structure in Central Argentina, using biparental markers and comparing the results with those previously obtained by us with mitochondrial DNA (mtDNA) in the same samples. A total of 521 healthy unrelated individuals living in 13 villages of the Córdoba and San Luis provinces were tested. The individuals were genotyped for ten autosomal ancestry informative markers (AIMs). Allele frequencies were compared with those of African, European and Native American populations, chosen to represent parental contributions. The AIM estimates indicated a greater influence of the Native American ancestry as compared to previous studies in the same or other Argentinean regions, but smaller than that observed with the mtDNA tests. These differences can be explained, respectively, by different genetic contributions between rural and urban areas, and asymmetric gene flow occurred in the past. But a most unexpected finding was the marked interpopulation genetic homogeneity found in villages located in diverse geographic environments across a wide territory, suggesting considerable gene flow. 相似文献
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Beatriz Sánchez-Nieto Maria Romero-Expósito José Antonio Terrón Leticia Irazola Marta Paiusco Elisabetta Cagni Caterina Ghetti Silvano Filice Francisco Sánchez-Doblado 《Reports of Practical Oncology and Radiotherapy》2018,23(4):251-259
The aim of this work was to estimate peripheral neutron and photon doses associated with the conventional 3D conformal radiotherapy techniques in comparison to modern ones such as Intensity modulated radiation therapy and volumetric modulated arc therapy. Assessment in terms of second cancer incidence ought to peripheral doses was also considered. For that, a dosimetric methodology proposed by the authors has been applied beyond the region where there is no CT information and, thus, treatment planning systems do not calculate and where, nonetheless, about one third of second primary cancers occurs. 相似文献
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Bruce R. Branchini Thomas M. Marschner Angelina M. Montemurro 《Analytical biochemistry》1980,104(2):386-396
A method is described for purifying luciferase from firefly lanterns in very good yield. The enzyme was extracted from Photinus lanterns, dialyzed, and further purified by affinity chromatography with 6-aminohexanoic acid-Sepharose 4b benzylamine. Fractions containing luciferase activity were pooled, concentrated by ultrafiltration, and crystallized by dialysis against a low-ionic-strength buffer. The crystalline enzyme appears to be homogeneous by sodium dodecyl sulfate-gel electrophoresis. The entire protocol described was conveniently performed in less than 3 days and yielded 6.2 mg of enzyme from 2.2 g of firefly lanterns. Furthermore, the affinity column employed can be reused at least five times without appreciable loss of binding capacity. Luciferase was chromatographed with several other affinity gels and the results are compared. It appears that 6-aminohexanoic acid-Sepharose 4b benzylamine does not function as a true bioaffinity gel, but rather as a hydrophobic interaction support. 相似文献
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Dana Pfefferle Angelina V. Ruiz-Lambides Anja Widdig 《Proceedings. Biological sciences / The Royal Society》2014,281(1774)
Widespread evidence exists that when relatives live together, kinship plays a central role in shaping the evolution of social behaviour. Previous studies showed that female rhesus macaques (Macaca mulatta) recognize familiar maternal kin using vocal cues. Recognizing paternal kin might, however, be more difficult as rhesus females mate promiscuously during the possible conception period, most probably concealing paternity. Behavioural observations indicate that semi free-ranging female rhesus macaques prefer to associate with their paternal half-sisters in comparison to unrelated females within the same group, particularly when born within the same age cohort. However, the cues and mechanism/s used in paternal kin discrimination remain under debate. Here, we investigated whether female rhesus macaques use the acoustic modality to discriminate between paternal half-sisters and non-kin, and tested familiarity and phenotype matching as the underlying mechanisms. We found that test females responded more often to calls of paternal half-sisters compared with calls of unrelated females, and that this discrimination ability was independent of the level of familiarity between callers and test females, which provides, to our knowledge, the first evidence for acoustic phenotype matching. Our study strengthens the evidence that female rhesus macaques can recognize their paternal kin, and that vocalizations are used as a cue. 相似文献
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Long‐term effects of tetanus toxoid inoculation on the demography and life expectancy of the Cayo Santiago rhesus macaques 下载免费PDF全文