Treatment of Lime Witches’ Broom Phytoplasma-Infected Mexican Lime with a Resistance Inducer and Study of its Effect on Systemic Resistance

Journal of Plant Growth Regulation - Destruction caused by plant diseases is terrible. Mexican lime witches' broom (MLWB) is a phytoplasma disease, which has caused considerable damage...     

Current progress in cancer immunotherapy based on natural killer cells

One of the most common diseases in the present era is cancer. The common treatment methods used to control cancer include surgery, chemotherapy, and radiotherapy. Despite progress in the treatment of cancers, there still is no definite therapeutic approach. Among the currently proposed strategies, immunotherapy is a new approach that can provide better outcomes compared with existing therapies. Employing natural killer (NK) cells is one of the means of immunotherapy. As innate lymphocytes, NK cells are capable of rapidly responding to cancer cells without being sensitized or restricted to the cognate antigen in advance, as compared to T cells that are tumor antigen-specific. Latest insights into the biology of NK cells have clarified the underlying molecular mechanisms of NK cell maturation and differentiation, as well as controlling their effector functions through the investigation of the ligands and receptors engaged in recognizing cancer cells by NK cells. Elucidating the fact that NK cells recognize cancer cells could similarly show the mechanism through which cancer cells possibly avoid NK cell-dependent immune surveillance. Additionally, the expectations for novel immunotherapies by targeting NK cells have increased through the latest clinical outcomes of T–cell-targeted cancer immunotherapy. For this emerging method, researchers are still attempting to develop protocols for conferring the best proliferation and expansion medium, activation pathways, utilization dosage, transferring methods, as well as reducing possible side effects in cancer therapy. This study reviews the NK cells, their proliferation and expansion methods, and their recent applications in cancer immunotherapy.     

Lentiviral Expression of Rabies Virus Glycoprotein in the Rat Hippocampus Strengthens Synaptic Plasticity

Rabies virus (RABV) is a neurotropic virus exclusively infecting neurons in the central nervous system. RABV encodes five proteins. Among them, the viral glycoprotein (RVG) plays a key role in viral entry into neurons and rabies pathogenesis. It was shown that the nature of the C-terminus of the RABV G protein, which possesses a PDZ-binding motif (PBM), modulates the virulence of the RABV strain. The neuronal protein partners recruited by this PBM may alter host cell function. This study was conducted to investigate the effect of RVG on synaptic function in the hippocampal dentate gyrus (DG) of rat. Two μl (10⁸ T.U./ml) of the lentiviral vector containing RVG gene was injected into the DG of rat hippocampus. After 2 weeks, the rat’s brain was cross-sectioned and RVG-expressing cells were detected by fluorescent microscopy. Hippocampal synaptic activity of the infected rats was then examined by recording the local field potentials from DG after stimulation of the perforant pathway. Short-term synaptic plasticity was also assessed by double pulse stimulation. Expression of RVG in DG increased long-term potentiation population spikes (LTP-PS), whereas no facilitation of LTP-PS was found in neurons expressing δRVG (deleted PBM). Furthermore, RVG and δRVG strengthened paired-pulse facilitation. Heterosynaptic long-term depression (LTD) in the DG was significantly blocked in RVG-expressing group compared to the control group. This blockade was dependent to PBM motif as rats expressing δRVG in the DG-expressed LTD comparable to the RVG group. Our data demonstrate that RVG expression facilitates both short- and long-term synaptic plasticity in the DG indicating that it may involve both pre- and postsynaptic mechanisms to alter synaptic function. Further studies are needed to elucidate the underlying mechanisms.

     

First report of c. 1499G>C mutation in a 6-month-child with cystic fibrosis

So far, more than 1800 mutations identified in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. In this case report, we presented first report of c. 1499G>C mutation in a 6-month-old girl with cystic fibrosis (CF) diagnosis. A 6-month-old girl with weakness and meconium Ileus referred to the pediatric clinic in Ilam, in the west of Iran. Patient''s skin was dark and suffered from bronchiectasis. The sweat test was performed, and the concentration of chloride and sodium in patient''s sweat was 130-135 mmol/L and 125-128 mmol/L, respectively. The exon 10 mutation analysis of a CF patient was performed. CFTR mutation analysis revealed the identification of 2 mutations in patient, the mutations were p.F508del (ΔF508) and c. 1499G>C (cd500), respectively. The mutation c. 1499G>C (cd500) were found for the first time in the world. Assessing this mutation in future study and genetic investigation is recommended.     

Magnetic immobilization of bacteria using iron oxide nanoparticles

Bacterial cell immobilization is a novel technique used in many areas of biosciences and biotechnology. Iron oxide nanoparticles have attracted much attention in bacterial cell immobilization due to their unique properties such as superparamagnetism, large surface area to volume ratio, biocompatibility and easy separation methodology. Adhesion is the basis behind many immobilization techniques and various types of interactions determine bacterial adhesion. Efficiency of bacterial cell immobilization using iron oxide nanoparticles (IONs) generally depends on the physicochemical properties of the IONs and surface properties of bacterial cells as well as environmental/culture conditions. Bacteria exhibit various metabolic responses upon interaction with IONs, and the potential applications of iron oxide nanoparticles in bacterial cell immobilization will be discussed in this work.     

Chlorogenic acid protective effects on paraquat-induced pulmonary oxidative damage and fibrosis in rats

Paraquat (PQ) is a widely used herbicide that can cause severe oxidative and fibrotic injuries in lung tissue. Due to the antioxidant and anti-inflammatory properties of chlorogenic acid (CGA), the present study investigated its effects on PQ-induced pulmonary toxicity. To this end, 30 male rats were randomly categorized into five groups of six. Initially, the first and third groups were treated intraperitoneally (IP) with normal saline and CGA (80 mg/kg) for 28 consecutive days, respectively. The second, fourth, and fifth groups were treated with normal saline and 20 and 80 mg/kg of CGA for 28 consecutive days, respectively, and received a single dose of PQ (IP, 20 mg/kg) on Day 7. Then, the animals were anesthetized with ketamine and xylazine, and lung tissue samples were collected for biochemical and histological examinations. The results showed that PQ significantly increased hydroxyproline (HP) and lipid peroxidation (LPO) and decreased the lung tissue antioxidant capacity. In addition, myeloperoxidase (MPO) activity increased significantly, while glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) activity declined substantially. The administration of therapeutic doses of CGA could prevent the oxidative, fibrotic, and inflammatory effects of PQ-induced lung toxicity, and these changes were consistent with histological observations. In conclusion, CGA may improve the antioxidant defense of lung tissue and prevent the spread of inflammation and the development of PQ-induced fibrotic injuries by enhancing antioxidant enzymes and preventing inflammatory cell infiltration.     

Reducing Soil Permeability Using Microbial Induced Carbonate Precipitation (MICP) Method: A Case Study of Shiraz Landfill Soil

Abstract

Improvement of engineering properties of soils to meet project requirements has long been subject of interest to civil engineers. One of the environment-friendly methods that have recently been used for this purpose is the biological method. These methods that actually benefit from various sciences such as biology, biochemistry, and civil engineering, use biological products or organisms such as bacteria that are commonly found in soils. In this study, the reduction of permeability or hydraulic conductivity of Shiraz landfill base soil using microbial induced calcite precipitation (MICP) has been explored. B. sphaericus was used to treat the soil. Falling head permeability tests are conducted to measure soil samples’ permeability before and after biological treatment. The target variables were the curing time, bacterial density, optimal nutrient content, and soil unit weight. The test results demonstrated that the permeability of the samples treated with Bacillus sphaericus decreases by increasing curing time, the density of calcium chloride solution and bacterial density of samples. This study showed that the MICP can be utilized as a new environment-friendly method for reducing the soil permeability at the base and walls of the landfill to form a barrier between the waste and the groundwater and substrata.     

Glossopharyngeal neuralgia secondary to vascular compression in a patient with multiple sclerosis: a case report

ABSTRACT: Glossopharyngeal neuralgia is an uncommon, painful syndrome, characterized by paroxysmsof pain in the sensory distribution of the 9th cranial nerve. Idiopathic glossopharyngealneuralgia may be due to compression of the glossopharyngeal nerve by adjacent vessels,while secondary glossopharyngeal neuralgia is associated with identifiable lesions affectingthe glossopharyngeal nerve at different levels of its neuroanatomic pathway.Glossopharyngeal neuralgia is rare in the general population, but is more common in patientswith multiple sclerosis. CASE PRESENTATION: A 56-year-old Caucasian woman with multiple sclerosis and migraine presented to ourfacility with intermittent lancinating pain to the right of her throat, tongue, and the floor ofher mouth that had been occurring for the past year. The pain was intense, sharp, andstabbing, which lasted two to six seconds with radiation to the right ear. Initially, the attackswere infrequent, however, they had become more intense and frequent over time. Our patientreported weight loss, headache, painful swallowing, and the inability to maintain sleep due topainful attacks. A neurological examination revealed a right-handed woman with triggerpoints in the back of the tongue and throat on the right side. She also had dysphagia,hoarseness, and pain in the distribution of the right glossopharyngeal nerve. Mild righthemiparesis, hyperreflexia, dysmetria, and an ataxic gait were present. A magnetic resonanceimaging scan of the brain was consistent with multiple sclerosis and magnetic resonanceangiography demonstrated a loop of the posterior inferior cerebellar artery compressing theright glossopharyngeal nerve. She responded satisfactorily to carbamazepine. Microvasculardecompression and Gamma Knife? radiosurgery were discussed in case of failure of themedical treatment; however, she declined these options. CONCLUSIONS: Glossopharyngeal neuralgia in multiple sclerosis may occur due to vascular compressivelesions and it should not be solely attributed to the underlying demyelinating process.Vascular compression of the glossopharyngeal nerve could independently causeglossopharyngeal neuralgia in patients with multiple sclerosis, and vascular imaging toexclude such a diagnosis is recommended.