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81.
Nakagawa A Nagaosa K Hirose T Tsuda K Hasegawa K Shiratsuchi A Nakanishi Y 《Development, growth & differentiation》2004,46(3):283-298
Class B scavenger receptor type I (SR-BI), a multiligand membrane protein, exists in various organs and cell types. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. Unlike interstitially localized Leydig cells, Sertoli cells present within the seminiferous tubules keep contact with spermatogenic cells and form the tight junction to divide the seminiferous epithelium into the basal and adluminal compartments. In this study, the expression and function of SR-BI in rat Sertoli cells were examined with respect to dependency on the spermatogenic cycle, the plasma membrane polarity, and the pituitary hormone follicle-stimulating hormone (FSH). When the expression of SR-BI was histochemically examined with testis sections, both protein and mRNA were already present in Sertoli cells during the first-round spermatogenesis and continued to be detectable thereafter. The level of SR-BI mRNA expression in Sertoli cells was lower at spermatogenic stages I-VI than at other stages. SR-BI was present and functional (in mediating cellular incorporation of lipids of high density lipoprotein) at both the apical and basolateral surfaces of polarized Sertoli cells. Finally, SR-BI expression at both the protein and mRNA levels was stimulated by FSH in cultured Sertoli cells. These results indicate that SR-BI functions on both the apical and basolateral plasma membranes of Sertoli cells, and that SR-BI expression in Sertoli cells changes during the spermatogenic cycle and is stimulated, at least in cultures, by FSH. 相似文献
82.
83.
Kadono S Sakamoto A Kikuchi Y Oh-eda M Yabuta N Koga T Hattori K Shiraishi T Haramura M Kodama H Esaki T Sato H Watanabe Y Itoh S Ohta M Kozono T 《Biochemical and biophysical research communications》2004,324(4):1227-1233
The 3D structure of human factor VIIa/soluble tissue factor in complex with a peptide mimetic inhibitor, propylsulfonamide-D-Thr-Met-p-aminobenzamidine, is determined by X-ray crystallography. As compared with the interactions between thrombin and thrombin inhibitors, the interactions at S2 and S3 sites characteristic of factor VIIa and factor VIIa inhibitors are revealed. The S2 site has a small pocket, which is filled by the hydrophobic methionine side chain in P2. The small S3 site fits the small size residue, D-threonine in P3. The structural data and SAR data of the peptide mimetic inhibitor show that these interactions in the S2 and S3 sites play an important role for the improvement of selectivity versus thrombin. The results will provide valuable information for the structure-based drug design of specific inhibitors for FVIIa/TF. 相似文献
84.
G protein-coupled receptors have a common structural motif of seven transmembrane alpha-helices and are classified into different families showing no sequence similarity. Extensive studies have been conducted on the structure-function relationship in family 1 receptors, but those in other families have not been well studied. In this study, to investigate the molecular basis leading to the G protein activation by metabotropic glutamate receptor (mGluR), the member of family 3, we searched for the amino acid residues responsible for the G protein activation in the second cytoplasmic loop, which was thought to be the main G protein binding region. Analyses of the systematical mutations of Gi/Go-coupled mGluR8 revealed the presence of a constitutively active mutation in the C-terminal region of the second loop. The corresponding mutation in the second loop of Gq-coupled mGluR1 also exhibited high agonist-independent activity. These results indicate that there is a common constitutive active mutation site regardless of mGluR subtypes, suggesting that the structural change of the junction between the second cytoplasmic loop and helix IV is strongly linked to the formation of the active state. 相似文献
85.
Ding QF Hayashi T Packiasamy AR Miyazaki A Fukatsu A Shiraishi H Nomura T Iguchi A 《Life sciences》2004,75(26):3185-3194
Although endothelial dysfunction deteriorates diabetic angiopathy, the mechanisms are obscure. We revealed that high glucose augmented eNOS through stimulation of eNOS mRNA in cultured BAECs. NO was decreased and O2- was increased simultaneously. NOS inhibitor, inhibited O2- release, so did NADPH oxidase inhibitor. The effects were synergistic. Both intracellular BH4 level and GTPCH1 activity were decreased by high glucose, in line with decrease of GTPCH1 mRNA. HMG-CoA reductase inhibitor, atorvastatin increased GTPCH1 mRNA and activity, and BH4 level. Conclusively, high glucose leads to eNOS dysfunction by inhibiting BH4 synthesis and atorvastatin stimulate BH4 synthesis directly, and it may work as atherogenic process. 相似文献
86.
Shigeru Yamada Eiichiro Uchimura Takanori Ueda Fukumi Iguchi Yutaka Akiyama Satoshi Fujita Masato Miyake Jun Miyake 《NanoBioTechnology》2006,2(3-4):95-100
We propose a simple procedure for the identification and quantitative analysis of neurite outgrowth in neuronal cell lines
that were uniformly differentiated. Upon stimulation most neuronal cell lines extend neurites in the differentiation process,
resulting, according to our observation, in the increase of cell surface area. This increase is dependent on the length and
the number of extended neurites. Furthermore, we use this method for the phenotype analysis of cell array experiments to perform
large-scale functional evaluation of genes involved in the neurite outgrowth during neuronal differentiation. 相似文献
87.
Takashi Nagata Mitsumasa Koyanagi Hisao Tsukamoto Akihisa Terakita 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2010,196(1):51-59
Peropsin, a member of the opsin family, has characteristics of two functionally distinct opsin-groups, that is, amino acid
residues conserved among opsins for light-sensing and a retinal-photoisomerase-like molecular property. Although such a bilateral
feature of peropsin seems to be important for understanding the diversity of the opsin family, previous studies have been
limited to higher deuterostome, vertebrate and amphioxus peropsins. Here, we report a protostome peropsin homologue from a
jumping spider. We found a spider opsin that shares amino acid homology and conserved amino acid residues with known peropsins.
The spider opsin-based pigment heterologously expressed in cultured cells exhibited photoisomerase-like isomerization characteristics
and a bistable nature. Based on the characteristics of both the amino acid homology and its photochemical properties, we concluded
that the spider opsin is the first protostome peropsin homologue. These results show that peropsin existed before the deuterostome–protostome
split like other members of the opsin family. In addition, the spider peropsin was localized to non-visual cells in the retina,
and fluorescence from reduced retinal chromophore was also observed in the region where peropsin was localized. These findings
provide the first demonstration that the peropsin can form a photosensitive pigment in vivo and underlie non-visual function. 相似文献
88.
Maki K Watabe E Iguchi Y Nakamura H Tomishima M Ohki H Yamada A Matsumoto S Ikeda F Tawara S Mutoh S 《Microbiology and immunology》2006,50(4):281-292
To establish an in vitro method of predicting in vivo efficacy of antifungal drugs against Candida albicans and Aspergillus fumigatus, the antifungal activities of fluconazole, itraconazole, and amphotericin B were determined in mouse serum. The minimum inhibitory concentration (MIC) of each drug was measured using mouse serum as a diluent. For C. albicans, the assay endpoint of azoles was defined as inhibition of mycelial extension (mMIC) and for A. fumigatus, as no growth (MIC). The MICs of amphotericin B for both pathogens were defined as the MIC at which no mycelial growth occurred. Serum MIC or mMIC determinations were then used to estimate the concentration of the drugs in serum of mice treated with antifungal drugs by multiplying the antifungal titer of the serum samples by the serum (m)MIC. The serum drug concentrations were also determined by HPLC. The serum concentrations estimated microbiologically showed good agreement with those determined by HPLC, except for itraconazole. Analysis of the serum samples from itraconazole-treated mice by a sensitive bioautography revealed the presence of additional spots, not seen in control samples of itraconazole. The bioautography assay demonstrated that the additional material detected in serum from mice treated with itraconazole was an active metabolite of itraconazole. The data showed that the apparent reduction in the itraconazole serum concentration as determined by HPLC was the result of the formation of an active metabolite, and that the use of a microbiological method to measure serum concentrations of drugs can provide a method for prediction of in vivo efficacy of antifungal drugs. 相似文献
89.
Ohnishi R Ito H Iguchi A Shinomiya K Kamei C Hatano T Yoshida T 《Bioscience, biotechnology, and biochemistry》2006,70(10):2560-2563
Chlorogenic acid possessed a weak caffeine-like psychostimulant property when assessed for its effect on spontaneous locomotor activity in mice. In the evaluation of the effects for the major metabolites of chlorogenic acid which were detected upon incubation with rat feces and/or excreted in urine after oral administration to rats, caffeic and m-coumaric acids were found to be the principal active metabolites, while the others contributed little to this caffeine-like psychostimulant activity. 相似文献
90.