全文获取类型
收费全文 | 2607篇 |
免费 | 139篇 |
出版年
2023年 | 6篇 |
2022年 | 17篇 |
2021年 | 44篇 |
2020年 | 15篇 |
2019年 | 26篇 |
2018年 | 39篇 |
2017年 | 31篇 |
2016年 | 46篇 |
2015年 | 74篇 |
2014年 | 73篇 |
2013年 | 180篇 |
2012年 | 161篇 |
2011年 | 167篇 |
2010年 | 104篇 |
2009年 | 101篇 |
2008年 | 172篇 |
2007年 | 183篇 |
2006年 | 154篇 |
2005年 | 126篇 |
2004年 | 199篇 |
2003年 | 150篇 |
2002年 | 125篇 |
2001年 | 38篇 |
2000年 | 37篇 |
1999年 | 42篇 |
1998年 | 39篇 |
1997年 | 34篇 |
1996年 | 21篇 |
1995年 | 19篇 |
1994年 | 14篇 |
1993年 | 18篇 |
1992年 | 33篇 |
1991年 | 39篇 |
1990年 | 13篇 |
1989年 | 26篇 |
1988年 | 31篇 |
1987年 | 12篇 |
1986年 | 15篇 |
1985年 | 7篇 |
1984年 | 14篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 8篇 |
1979年 | 16篇 |
1978年 | 5篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1974年 | 5篇 |
1967年 | 8篇 |
1965年 | 5篇 |
排序方式: 共有2746条查询结果,搜索用时 15 毫秒
21.
Takao Tsuji Akihiro Ogawa Takashi Miyata Tatsuya Iida Takeshi Honda Toshio Miwatani 《FEMS microbiology letters》1986,37(3):345-350
Abstract A mutant (TUH No. 9) of a porcine strain of enterotoxigenic Escherichia coli (ETEC) produces as abnormal B subunit (B') of heat-labile enterotoxin (LT), which has aspartate instead of glycine at residue 33 from the N-terminus and does not bind to the receptor, GM1 ganglioside. The antigenicities of the receptor-binding site of LT were analyzed.
The antibody, which could not bind to the B' subunit in the anti-B subunit of porcine LT(LTp)-serum, could bind to cholera toxin (CT), LTp and LT produced by a human ETEC strain (LTh), suggesting that it recognizes a common epitope of LTp, LTh and CT. Thus glycine at residue 33 from the N-terminus in the B subunit of CT, LTh and LTp may be related to the common epitope of these three toxins. The bindings of CT, LTh and LTp to the antibody were inhibited by the GM1 ganglioside.
These data indicate that the antibody recognizes a common epitope in the receptor (GM1 ganglioside)-binding site of CT, LTh and LTp. 相似文献
The antibody, which could not bind to the B' subunit in the anti-B subunit of porcine LT(LTp)-serum, could bind to cholera toxin (CT), LTp and LT produced by a human ETEC strain (LTh), suggesting that it recognizes a common epitope of LTp, LTh and CT. Thus glycine at residue 33 from the N-terminus in the B subunit of CT, LTh and LTp may be related to the common epitope of these three toxins. The bindings of CT, LTh and LTp to the antibody were inhibited by the GM1 ganglioside.
These data indicate that the antibody recognizes a common epitope in the receptor (GM1 ganglioside)-binding site of CT, LTh and LTp. 相似文献
22.
23.
In an effort to obtain orange mutants ofBlastocladiella emersonii
Cantino &Hyatt, wild type zoospores were treated with mitomycin. From the variants produced, we obtained a stable, albino mutant (Ma-1) that differs significantly from another, previously described (Shaw &Cantino, 1969) UV-induced, albino variant. This report concerns the origin of Ma-1, its distinguishing features, and its apparent similarity to the few LC (late colorless) plants that normally appear in wild type populations. A preliminary note regarding mitomycin-induced variants ofB. emersonii has been published (Matsumae &Cantino, 1970). 相似文献
24.
Growth and other characteristics of a cell line (SP-8) established from Shope virus-induced cutaneous papilloma of domestic rabbit 总被引:1,自引:0,他引:1
25.
26.
H Chen S Shoumura S Emura M Utsumi T Yamahira H Isono 《Histology and histopathology》1990,5(4):477-484
Ultrastructural changes of the parathyroid glands of pinealectomized golden hamsters were investigated. The main changes in the parathyroid glands 1 hour and 1 day after pinealectomy compared with the control and sham-operated groups were an increase of the Golgi complexes, cisternae of the granular endoplasmic reticulum and large vacuolar bodies. In addition, many chief cells contained numerous prosecretory granules in the Golgi areas and many secretory granules in the peripheral cytoplasm. The morphology of the parathyroid glands 7 and 30 days after pinealectomy resembled that of the control parathyroid glands. These results suggest that pinealectomy affects the secretory activity of the parathyroid gland. 相似文献
27.
In vitro fertilization in the domestic fowl (Gallus domesticus) was investigated by observation of the early nuclear events. Ova retrieved from the fimbria following ovulation were inseminated in vitro with 10(6)-10(7) spermatozoa in Dulbecco's modified Eagle's medium (DMEM) for 10 min and then further incubated in DMEM + albumen for 1, 2, 3, or 4 hr. These eggs were histologically examined by epifluorescent microscopy after staining with 4',6'-diamidino-2-phenylindole (DAPI). Nuclei of spermatozoa at various stages of transformation were observed in the ova incubated for 1-3 hr. Close pairing of two pronuclei, presumed to be male and female juxtaposition, was detected in ova incubated for 4 hr. These data provide direct evidence for the in vitro fertilization of fowl eggs and suggested that the early process of in vitro fertilization is comparable to that of in vivo fertilization. 相似文献
28.
Takeshi Minami Masayo Ichii Yoshiyuki Tohno Setsuko Tohno Masako Utsumi Masa-oki Yamada Yuko Okazaki 《Biological trace element research》1996,55(1-2):199-205
The purpose of this study was to determine the extent of aluminum (Al) accumulation in the human aorta and cerebral arteries.
The Al contents in the aortae and in the cerebral arteries from 23 human subjects was determined by inductively coupled plasma
atomic emission spectrophotometry (ICP-AES). The subjects' age range was 45–99-yr-old; 15 of the subjects were males and 8
were females. Al was detected in twelve aortae and in six cerebral arteries, when the entire specimen was analyzed. Two specimens
where Al was found in the cerebral arteries contained no Al in the aorta. No relationship to the subject's sex was found.
When related to age, two groups were established. Group L (45–75 yr old) and group H (>75 yr old), which exhibited aortal
Al concentrations of 33.3 and 72.7%, respectively. When the aortic wall was dissected into the tunica intima, media, and adventitia,
Al was found mainly in the tunica media. In the aorta, significant relationships were found between Al and phosphorus (P)
levels (r=0.801,p<0.01) and between Al and calcium (Ca) (r=0.661,p<0.05). We have concluded that Al accumulation is age-dependent and that it occurs both in the aorta and in the cerebral artery.
In the aorta, accumulation occurs mainly in the tunica media. Both P and Ca appear to enhance aortal Al accumulation. 相似文献
29.
Akihiro Ohira Eugene de Juan Jr Yasuo Tano Charles A. Wilson 《The Histochemical journal》1996,28(9):607-611
Summary It has been proposed that basic fibroblast growth factor (basic FGF) mediates the neovascular response in a variety of conditions,
including diabetic retinopathy and branch retinal vein occlusion. To test the hypothesis that basic FGF was released from
retinal stores as a result of retinal ischaemia, transient retinal ischaemia was induced, followed by 48 h of reperfusion,
in the rat by combined central retinal vasculature and optic nerve ligation. The immunolocalization of basic FGF was studied
in the retina. We found that basic FGF in the normal retina is present around the deeper retinal vessels and in the neuronal
tissue of the outer plexiform layer. In the eyes that had ischaemia followed by reperfusion, there was moderate cellular oedema
with retinal swelling, and mitoses in the inner nuclear and plexiform layers. There were no changes evident at the immunohistochemical
level either in the intensity or distribution of stores of basic FGF. We conclude from these data that stores of basic FGF
are not altered dramatically under the conditions of transient experimental ischaemia and reperfusion in the rat, despite
the presence of cellular proliferation. 相似文献
30.