全文获取类型
收费全文 | 5186篇 |
免费 | 657篇 |
国内免费 | 3篇 |
出版年
2021年 | 70篇 |
2018年 | 71篇 |
2017年 | 52篇 |
2016年 | 109篇 |
2015年 | 146篇 |
2014年 | 170篇 |
2013年 | 173篇 |
2012年 | 268篇 |
2011年 | 246篇 |
2010年 | 146篇 |
2009年 | 148篇 |
2008年 | 174篇 |
2007年 | 201篇 |
2006年 | 183篇 |
2005年 | 188篇 |
2004年 | 146篇 |
2003年 | 174篇 |
2002年 | 170篇 |
2001年 | 180篇 |
2000年 | 171篇 |
1999年 | 130篇 |
1998年 | 74篇 |
1997年 | 54篇 |
1996年 | 62篇 |
1995年 | 50篇 |
1994年 | 66篇 |
1993年 | 47篇 |
1992年 | 108篇 |
1991年 | 114篇 |
1990年 | 96篇 |
1989年 | 124篇 |
1988年 | 85篇 |
1987年 | 107篇 |
1986年 | 80篇 |
1985年 | 92篇 |
1984年 | 76篇 |
1983年 | 60篇 |
1982年 | 59篇 |
1981年 | 51篇 |
1980年 | 50篇 |
1979年 | 72篇 |
1978年 | 48篇 |
1977年 | 51篇 |
1976年 | 46篇 |
1974年 | 58篇 |
1973年 | 58篇 |
1972年 | 68篇 |
1970年 | 51篇 |
1969年 | 54篇 |
1968年 | 46篇 |
排序方式: 共有5846条查询结果,搜索用时 182 毫秒
991.
The hypothesis that ovulation-inducing factor/nerve growth factor (OIF/NGF) isolated from llama seminal plasma exerts a luteotrophic effect was tested by examining changes in circulating concentrations of LH and progesterone, and the vascular perfusion of the ovulatory follicle and developing CL. Female llamas with a growing follicle of 8 mm or greater in diameter were assigned randomly to one of three groups (n = 10 llamas per group) and given a single intramuscular dose of PBS (1 mL), GnRH (50 μg), or purified OIF/NGF (1.0 mg). Cineloops of ultrasonographic images of the ovary containing the dominant follicle were recorded in brightness and power Doppler modalities. Llamas were examined every 4 hours from the day of treatment (Day 0) until ovulation, and every other day thereafter to Day 16. Still frames were extracted from cineloops for computer-assisted analysis of the vascular area of the preovulatory follicle from treatment to ovulation and of the growing and regressing phases of subsequent CL development. Blood samples were collected for the measurement of plasma LH and progesterone concentrations. The diameter of the dominant follicle at the time of treatment did not differ among groups (P = 0.48). No ovulations were detected in the PBS group but were detected in all llamas given GnRH or OIF/NGF (0/10, 10/10, and 10/10, respectively; P < 0.0001). No difference was detected between the GnRH and OIF/NGF groups in the interval from treatment to ovulation (32.0 ± 1.9 and 30.4 ± 5.7 hours, respectively; P = 0.41) or in maximum CL diameter (13.1 ± 0.4 and 13.5 ± 0.3 mm, respectively; P = 0.44). The preovulatory follicle of llamas treated with OIF/NGF had a greater vascular area at 4 hours after treatment than that of the GnRH group (P < 0.001). Similarly, the luteal tissue of llamas treated with purified OIF/NGF had a greater vascular area than that of the GnRH group on Day 6 after treatment (P < 0.001). The preovulatory surge in plasma LH concentration began, and peaked 1 to 2 hours later in the OIF/NGF group than in the GnRH group (P < 0.05). Plasma progesterone concentration was higher on Day 6 in the OIF/NGF group than in the GnRH group (P < 0.001). Results support the hypothesis that OIF/NGF exerts a luteotrophic effect by altering the secretion pattern of LH and enhancing tissue vascularization during the periovulatory period and early stages of CL development. 相似文献
992.
Dowling DJ Noone CM Adams PN Vukman KV Molloy SF Forde J Asaolu S O'Neill SM 《International journal for parasitology》2011,41(2):255-261
Dendritic cells (DCs) matured with helminth-derived molecules that promote Th2 immune responses do not follow conventional definitions of DC maturation processes. While a number of models of DC maturation by Th2 stimuli are postulated, further studies are required if we are to clearly define DC maturation processes that lead to Th2 immune responses. In this study, we examine the interaction of Th2-inducing molecules from the parasitic helminth Ascaris lumbricoides with the maturation processes and function of DCs. Here we show that murine bone marrow-derived DCs are partially matured by A. lumbricoides pseudocoelomic body fluid (ABF) as characterised by the production of IL-6, IL-12p40 and macrophage inflammatory protein 2 (MIP-2) but no enhanced expression of cluster of differentiation (CD)-14, T-cell co-stimulatory markers CD80, CD86, CD40, OX40L and major histocompatibility complex class II was observed. Despite these phenotypic characteristics, ABF-stimulated DCs displayed the functional hallmarks of fully matured cells, enhancing DC phagocytosis and promoting Th2-type responses in skin-draining lymph node cells in vivo. ABF activated Th2-associated extracellular signal-regulated kinase-1 and nuclear factor-kB intracellular signalling pathways independently of toll-like receptor 4. Taken together, we believe this is the first paper to demonstrate A. lumbricoides murine DC-Th cell-driven responses shedding further light on DC maturation processes by helminth antigens. 相似文献
993.
994.
Sensitivity of leaf size and shape to climate: global patterns and paleoclimatic applications 总被引:2,自引:0,他引:2
Peppe DJ Royer DL Cariglino B Oliver SY Newman S Leight E Enikolopov G Fernandez-Burgos M Herrera F Adams JM Correa E Currano ED Erickson JM Hinojosa LF Hoganson JW Iglesias A Jaramillo CA Johnson KR Jordan GJ Kraft NJ Lovelock EC Lusk CH Niinemets U Peñuelas J Rapson G Wing SL Wright IJ 《The New phytologist》2011,190(3):724-739
? Paleobotanists have long used models based on leaf size and shape to reconstruct paleoclimate. However, most models incorporate a single variable or use traits that are not physiologically or functionally linked to climate, limiting their predictive power. Further, they often underestimate paleotemperature relative to other proxies. ? Here we quantify leaf-climate correlations from 92 globally distributed, climatically diverse sites, and explore potential confounding factors. Multiple linear regression models for mean annual temperature (MAT) and mean annual precipitation (MAP) are developed and applied to nine well-studied fossil floras. ? We find that leaves in cold climates typically have larger, more numerous teeth, and are more highly dissected. Leaf habit (deciduous vs evergreen), local water availability, and phylogenetic history all affect these relationships. Leaves in wet climates are larger and have fewer, smaller teeth. Our multivariate MAT and MAP models offer moderate improvements in precision over univariate approaches (± 4.0 vs 4.8°C for MAT) and strong improvements in accuracy. For example, our provisional MAT estimates for most North American fossil floras are considerably warmer and in better agreement with independent paleoclimate evidence. ? Our study demonstrates that the inclusion of additional leaf traits that are functionally linked to climate improves paleoclimate reconstructions. This work also illustrates the need for better understanding of the impact of phylogeny and leaf habit on leaf-climate relationships. 相似文献
995.
Aranjuelo I Ebbets AL Evans RD Tissue DT Nogués S van Gestel N Payton P Ebbert V Adams WW Nowak RS Smith SD 《Oecologia》2011,167(2):339-354
During the first few years of elevated atmospheric [CO(2)] treatment at the Nevada Desert FACE Facility, photosynthetic downregulation was observed in desert shrubs grown under elevated [CO(2)], especially under relatively wet environmental conditions. Nonetheless, those plants maintained increased A (sat) (photosynthetic performance at saturating light and treatment [CO(2)]) under wet conditions, but to a much lesser extent under dry conditions. To determine if plants continued to downregulate during long-term exposure to elevated [CO(2)], responses of photosynthesis to elevated [CO(2)] were examined in two dominant Mojave Desert shrubs, the evergreen Larrea tridentata and the drought-deciduous Ambrosia dumosa, during the eighth full growing season of elevated [CO(2)] treatment at the NDFF. A comprehensive suite of physiological processes were collected. Furthermore, we used C labeling of air to assess carbon allocation and partitioning as measures of C sink activity. Results show that elevated [CO(2)] enhanced photosynthetic performance and plant water status in Larrea, especially during periods of environmental stress, but not in Ambrosia. δ(13)C analyses indicate that Larrea under elevated [CO(2)] allocated a greater proportion of newly assimilated C to C sinks than Ambrosia. Maintenance by Larrea of C sinks during the dry season partially explained the reduced [CO(2)] effect on leaf carbohydrate content during summer, which in turn lessened carbohydrate build-up and feedback inhibition of photosynthesis. δ(13)C results also showed that in a year when plant growth reached the highest rates in 5 years, 4% (Larrea) and 7% (Ambrosia) of C in newly emerging organs were remobilized from C that was assimilated and stored for at least 2 years prior to the current study. Thus, after 8 years of continuous exposure to elevated [CO(2)], both desert perennials maintained their photosynthetic capacities under elevated [CO(2)]. We conclude that C storage, remobilization, and partitioning influence the responsiveness of these desert shrubs during long-term exposure to elevated [CO(2)]. 相似文献
996.
Berland MA von Baer A Ruiz J Parraguez VH Morales P Adams GP Ratto MH 《Theriogenology》2011,75(8):1482-1488
The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with either: 1) 25 mg of FSH im, twice daily for 4 d; or 2) 1000 IU of eCG as a single i.m. dose. The start of gonadotropin treatment was considered Day 0. Both groups were given 5 mg of Armour Standard LH im on Day 6, and COC were collected by follicle aspiration on Day 7. Expanded COC collected from FSH- (n = 157) and eCG-treated llamas (n = 151) were fertilized in vitro using epididymal sperm, and presumptive zygotes were in vitro cultured in SOF medium for 8 d. The FSH and eCG treatment groups did not differ with respect to: the number of follicles ≥7 mm (16.0 ± 2.7 vs 14.0 ± 1.9, respectively; P = 0.5); the number of COC collected (11.5 ± 1.9 vs 9.7 ± 1.2; P = 0.4); the number of expanded COC (9.8 ± 1.4 vs 9.4 ± 1.2; P = 0.8); or the percentage of presumptive zygotes which developed into 2 to 8 cell stage embryos (65.3 vs 63.1), morulas (46.2 vs 42.5), or blastocysts (23.1 vs 20.5; P > 0.05). In conclusion, FSH and eCG treatments were equally effective for recovery of a high number of expanded COC which were used directly for in vitro fertilization. Furthermore, rate of embryo development was not significantly affected by the gonadotropin treatment used. 相似文献
997.
In vivo identification of tumor- suppressive PTEN ceRNAs in an oncogenic BRAF-induced mouse model of melanoma 总被引:6,自引:0,他引:6
998.
Polizzi V Fazzini L Adams A Picco AM De Saeger S Van Peteghem C De Kimpe N 《Microbial ecology》2011,62(4):838-852
A Penicillium decumbens strain was collected from a water-damaged building, and the production of microbial volatile organic compounds (MVOCs) was
investigated by means of headspace solid-phase microextraction, followed by GC-MS analysis. The strain was characterized by
a high production of (+)-thujopsene. The influence of various temperatures, relative humidity (RH) values, substrates, and
inoculum concentrations on fungal growth and (+)-thujopsene production was studied. The optimal temperature and relative humidity
for P. decumbens growth were 30°C and 100% RH, respectively. In general, the more favourable the incubation parameters were for growth, the
faster maximum (+)-thujopsene production was reached. Moreover, the antifungal activity of thujopsene was tested against 16
fungal strains. The growth of five of these fungal strains was negatively affected both by thujopsene alone and when grown
in contact with the MVOCs produced by P. decumbens. Following these results and since growth of P. decumbens itself was also inhibited by thujopsene, an autoregulatory function for this compound was proposed. Few data are present
in the literature about chemical communication between fungi. The present research could, therefore, contribute to understanding
fungal metabolism and behaviour in indoor environments. 相似文献
999.
Polymyxa spp. are obligate biotrophs belonging to the plasmodiophorid group, responsible for transmitting a large number of plant viruses to many crop species. Their obligate nature makes them difficult to study. Controlled environment experiments were used to investigate the potential of infection of Arabidopsis thaliana by Polymyxa spp. to provide a more tractable system. Two ecotypes of Arabidopsis, Columbia and Landsberg erecta, were grown in soils known to be infested with Polymyxa. At the end of a 2-month growth period, both ecotypes were found to harbour Polymyxa-like structures or spores. These findings were confirmed by Polymyxa-specific PCR tests and rDNA sequencing, which positively identified the presence of Polymyxa in the roots of both ecotypes of Arabidopsis. Both Polymyxa graminis and Polymyxa betae were identified. This is the first report of infection of Arabidopsis by Polymyxa spp. and shows the possibility of using this system for studies of infection biology and host-parasite interactions. 相似文献
1000.
David Cote Blair K. Adams Keith D. Clarke Mervin Langdon 《Environmental Biology of Fishes》2011,92(3):351-360
Lake morphometry and water chemistry were analyzed as predictors of brook trout and total salmonid biomass (brook trout, Atlantic
salmon and Arctic char) in water bodies of Newfoundland. Lake morphometric variables included surface area, depth, perimeter
and catchment area while water chemistry variables included conductivity/TDS and total Phosphorus. The broadly used Morpho-Edaphic
Index was also assessed. Fish biomass/ha varied by an order of magnitude in the study lakes, despite similar water chemistry.
Interactive stepwise multiple regression analysis suggested that Basin Permanence Index best explains brook trout biomass
while surface area best explains the variance of total salmonid biomass in the relatively small, unproductive water bodies
of Insular Newfoundland. In contrast, the Morphoedaphic Index performed poorly. The results suggest that biomass relationships
with easily measured habitat variables such as surface area provide useful information, especially in management of water
bodies under similar climate and nutrient regimes. 相似文献