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排序方式: 共有139条查询结果,搜索用时 171 毫秒
51.
Abdolreza Daneshvar Amoli Nazanin Mohebali Parvaneh Farzaneh Seyed Abolhassan Shahzadeh Fazeli Laleh Nikfarjam Sepideh Ashouri Movasagh Zahra Moradmand Meysam Ganjibakhsh Ahmad Nasimian Mehrnaz Izadpanah Faezeh Vakhshiteh Neda sadat Gohari Najmeh sadat Masoudi Maryam Farghadan Shiva Mohamadi Moghanjoghi Masoud Khalili Kourosh J. Khaledi 《In vitro cellular & developmental biology. Animal》2017,53(4):337-343
Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genetic resources. It will also create an accessible database for researchers. 相似文献
52.
Samaneh Reiszadeh Jahromi Mohammad Haddadi T. Shivanandappa S. R. Ramesh 《Neurochemical research》2013,38(12):2616-2624
In this paper, we have demonstrated for the first time, the antioxidant and neuroprotective effects of Decalepis hamiltonii (Dh) root extract against paraquat (PQ)-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Exposure of adult D. melanogaster (Oregon K) to PQ induced oxidative stress as evidenced by glutathione depletion, lipid peroxidation and enhanced activities of antioxidant enzymes such as catalase, superoxide dismutase as well as elevated levels of acetylcholine esterase. Pretreatment of flies by feeding with Dh extract (0.1, 0.5 %) for 14 days boosted the activities of antioxidant enzymes and prevented the PQ-induced oxidative stress. Dietary feeding of Dh extract prior to PQ exposure showed a lower incidence of mortality and enhanced motor activities of flies in a negative geotaxis assay; both suggesting the neuroprotective potential of Dh. Based on the results, we contemplate that the roots of Dh might prevent and ameliorate the human diseases caused by oxidative stress. The neuroprotective action of Dh can be attributed to the antioxidant constituents while the precise mechanism of its action needs further investigations. 相似文献
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55.
Jahromi EZ White W Wu Q Yamdagni R Gailer J 《Metallomics : integrated biometal science》2010,2(7):460-468
The development of an analytical method to quantify the major Cu, Fe and Zn-containing metalloproteins in mammalian plasma has been recently reported. This method is based on the separation of plasma proteins by size exclusion chromatography (SEC) followed by the on-line detection of the metalloproteins by an inductively coupled plasma atomic emission spectrometer (ICP-AES). To assess whether the mobile phase buffer can affect the SEC-ICP-AES-derived metalloproteome pattern, thawed rabbit plasma was analyzed using phosphate buffered saline (PBS)-buffer (0.15 M, pH 7.4), Tris-buffer (0.1 and 0.05 M, pH 7.4), Hepes-buffer (0.1 M, pH 7.4) or Mops-buffer (0.1 M, pH 7.4). In contrast to the Cu-specific chromatograms, the Fe and Zn-specific chromatograms that were obtained with Tris, Hepes and Mops-buffer were considerably different from those attained with PBS-buffer. The Tris, Hepes and Mops-buffer mediated redistribution of ~25% plasma Zn(2+) from <100 kDa to >100-600 kDa plasma proteins and to a smaller extent to a <10 kDa (Tris)(2)Zn(2+)-complex can be rationalized in terms of the abstraction of Zn(2+) from the weak binding site on albumin. In contrast, only Hepes and Mops-buffer redistributed ~20% of plasma Fe(3+) from the <100 kDa to the >600 kDa elution range. Based on these results and considering that the utilization of PBS-buffer has previously resulted in the detection of a number of Cu, Fe and Zn-containing metalloentities in rabbit plasma that was most consistent with literature data, this mobile phase buffer is recommended for metallomic studies regarding mammalian blood plasma. 相似文献
56.
Khodadadi H. Jahromi G. P. Zaeinalifard G. Fasihi-Ramandi M. Esmaeili M. Shahriary A. 《Neurophysiology》2020,52(2):124-133
Neurophysiology - Parkinson’s disease (PD) is a widespread neurological disorder mainly characterized by gradual death of dopaminergic neurons in the basal ganglia. In our study, we compared... 相似文献
57.
Ahmad Farhad Talebi Masoud Tohidfar Meisam Tabatabaei Abdolreza Bagheri Motahhareh Mohsenpor Seyed Kaveh Mohtashami 《Molecular biology reports》2013,40(7):4421-4428
Developing a reliable technique to transform unicellular green algae, Chlorella vulgaris, could boost potentials of using microalgae feedstock in variety of applications such as biodiesel production. Volumetric lipid productivity (VLP) is a suitable variable for evaluating potential of algal species. In the present study, the highest VLP level was recorded for C. vulgaris (79.08 mg l?1 day?1) followed by 3 other strains studied; C. emersonii, C. protothecoides, and C. salina by 54.41, 45 and 18.22 mg l?1day?1, respectively. Having considered the high productivity of C. vulgaris, it was selected for the preliminary transformation experiment through micro-particle bombardment. Plasmid pBI 121, bearing the reporter gene under the control of CaMV 35S promoter and the kanamycin marker gene, was used in cells bombardment. Primary selection was done on a medium supplemented by 50 mg l?1 kanamycin. After several passages, the survived cells were PCR-tested to confirm the stability of transformation and then were found to exhibit β-glucuronidase (GUS) activity in comparison with the control cells. Southern hybridization of npt II probe with genomic DNA revealed stable integration of the cassette in three different positions in the genome. The whole process was successfully implemented as a pre-step to transform the algal cells by genes involved in lipid production pathway which will be carried out in our future studies. 相似文献
58.
Mojtaba Ghane Jahromi Ali Asgar Pourmirza Golam Reza Sadeghi Seyed Ali Hoseini 《Archives Of Phytopathology And Plant Protection》2013,46(5):526-533
In this research, adults of Tribolium castaneum (Herbst) and Rhyzopertha dominica (Fabricius) were exposed to various air pressures solely (first phase) and air pressures together with acrolein (second phase) in various durations and mortality rates were determined. Complete mortality of T. castaneum was obtained at 1, 3 and 6 h after exposure to 10, 30 and 50 mmHg, respectively. R. dominica showed 96.25% and 60% mortality during 3 and 10 h exposed to 10 and 30 mmHg, respectively. Percentage mortality of the insects in second phase was significantly higher than the rate in first phase. T. castaneum was more susceptible than R. dominica in the both phases. 相似文献
59.
Farsad F Karbassi A Monavari SM Mortazavi MS Farshchi P 《Biological trace element research》2011,143(3):1828-1842
This study aims to investigate the pollution rate of heavy metals on the western seaboard of Bandarabbas in southeast Iran
using a new pollution index. The bulk of the analysis was conducted on sediments, followed by selection of a few samples to
perform experiments on chemical partitioning studies as well as biological accessibility. On this basis, the proportions of
natural and anthropogenic elements were calculated. Finally, with regard to chemical separation results, the pollution rate
was calculated based on Muller's geo-chemical index, enrichment factor, pollution index, and a new "Risk" index. Chemical
separation showed the anthropogenic origin of elements are as follows: Ni(27.5%) > Zn(6.5%) > Pb(2%) > Al (0.2%). The newly
developed pollution index is indicative of "low environmental pollution "for Ni. 相似文献
60.
The rupture of a cerebral aneurysm is the most common cause of subarachnoid hemorrhage. Endovascular embolization of the aneurysms by implantation of Guglielmi detachable coils (GDC) has become a major treatment approach in the prevention of a rupture. Implantation of the coils induces formation of tissues over the coils, embolizing the aneurysm. However, blood entry into the coiled aneurysm often occurs due to failures in the embolization process. Current diagnostic methods used for aneurysms, such as X-ray angiography and computer tomography, are ineffective for continuous monitoring of the disease and require extremely expensive equipment. Here we present a novel technique for wireless monitoring of cerebral aneurysms using implanted embolization coils as radiofrequency resonant sensors that detect the blood entry. The experiments show that commonly used embolization coils could be utilized as electrical inductors or antennas. As the blood flows into a coil-implanted aneurysm, parasitic capacitance of the coil is modified because of the difference in permittivity between the blood and the tissues grown around the coil, resulting in a change in the coil's resonant frequency. The resonances of platinum GDC-like coils embedded in aneurysm models are detected to show average responses of 224–819 MHz/ml to saline injected into the models. This preliminary demonstration indicates a new possibility in the use of implanted GDC as a wireless sensor for embolization failures, the first step toward realizing long-term, noninvasive, and cost-effective remote monitoring of cerebral aneurysms treated with coil embolization. 相似文献