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501.
Pernilla Östlund Kalle Kilk Maria Lindgren Mattias Hällbrink Yang Jiang Metka Budihna Katarina Cerne Aljosa Bavec Claes-Göran Östenson Matjaz Zorko Ülo Langel 《International journal of peptide research and therapeutics》2005,11(4):237-247
Cell-penetrating peptides have proven themselves as valuable vectors for intracellular delivery. Relatively little is known
about the frequency of cell-penetrating sequences in native proteins and their functional role. By computational comparison
of peptide sequences, we recently predicted that intracellular loops of G-protein coupled receptors (GPCR) have high probability
for occurrence of cell-penetrating motifs. Since the loops are also receptor and G-protein interaction sites, we postulated
that the short cell-penetrating peptides, derived from GPCR, when applied extracellularly can pass the membrane and modulate
G-protein activity similarly to parent receptor proteins. Two model systems were analyzed as proofs of the principle. A peptide
based on the C-terminal intracellular sequence of the rat angiotensin receptor (AT1AR) is shown to internalize into live cells
and elicit blood vessel contraction even in the presence of AT1AR antagonist Sar1-Thr8-angiotensin II. The peptide interacts with the same selectivity towards G-protein subtypes as agonist-activated AT1AR and
blockade of phospholipase C abolishes its effect. Another cell-penetrating peptide, G53-2 derived from human glucagon-like
peptide receptor (GLP-1R) is shown to induce insulin release from isolated pancreatic islets. The mechanism was again found
to be shared with the original GLP-1R, namely G11-mediated inositol 1,4,5-triphosphate release pathway. These data reveal a novel possibility to mimic the effects of signalling
transmembrane proteins by application of shorter peptide fragments. 相似文献
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A novel leukotriene produced by stimulation of leukocytes with formylmethionylleucylphenylalanine 总被引:5,自引:0,他引:5
W Jubiz O R?dmark C Malmsten G Hansson J A Lindgren J Palmblad A M Udén B Samuelsson 《The Journal of biological chemistry》1982,257(11):6106-6110
Stimulation of human polymorphonuclear leukocytes with the chemotactic peptide formylmethionylleucylphenylalanine led to the formation of a novel leukotriene: 5(S),12(R)-dihydroxy-6,8,10,14-eicosatetraen-1,20-dioic acid. This dihydroxydicarboxylic acid is derived from omega-oxidation of 5(S),12(R),dihydroxy-6,8,10,14-eicosatetradienoic acid (leukotriene B4). The intermediate 5(S),12(R),20-trihydroxy-6,8,10,14-eicosatetraenoic acid was also isolated from these incubations. The two metabolites of leukotriene B4 exhibit chemotactic properties for human polymorphonuclear leukocytes but are less active in this respect than the parent compound. 相似文献
506.
Density gradient ultracentrifugation of human serum high density lipoproteins (HDL) from both normolipemic males and females results in a distribution of HDL concentration versus subfraction hydrated density which has three maxima. Gradient gel electrophoresis of total HDL is characterized by three banding maxima, the positions of which suggest the presence of three particle size ranges: I. 10.8-12.0 nm, II. 9.7-10.7 nm, and III. 8.5-9.6 nm. Gradient gel electrophoresis of density gradient subfractions established an inverse relationship between particle size and particle hydrated density which was corroborated by electron microscopy and analytic ultracentrifugation. Comparison of male HDL from size ranges I, II, and III with female HDL from the same size ranges showed only small differences in the mean value of the peak F degrees 1.20 rate, size, molecular weight, protein weight percent, and weight protein/weight phospholipid. Major differences between males and females were seen in the relative amounts of HDL in density gradient subfractions 1-3 (size range I material) and 11-12 (size range III material); the percent total HDL in the group of subfractions 1-3 was greatly increased in female HDL while that of the group of subfractions 11-12 was increased in the male HDL. These studies indicate the presence of at least three major components in HDL instead of two (HDL2 and HDL3) and that peak F degrees 1.20 rate differences in HDL schlieren patterns between males and females are a function of the relative levels of these three components. 相似文献
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Nasa Sinnott-Armstrong Isabel S. Sousa Samantha Laber Elizabeth Rendina-Ruedy Simon E. Nitter Dankel Teresa Ferreira Gunnar Mellgren David Karasik Manuel Rivas Jonathan Pritchard Anyonya R. Guntur Roger D. Cox Cecilia M. Lindgren Hans Hauner Richard Sallari Clifford J. Rosen Yi-Hsiang Hsu Eric S. Lander Melina Claussnitzer 《Cell metabolism》2021,33(3):615-628.e13
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To pace or not to pace: a pilot study of four‐ and five‐gaited Icelandic horses homozygous for the DMRT3 ‘Gait Keeper’ mutation 下载免费PDF全文
K. Jäderkvist Fegraeus I. Hirschberg T. Árnason L. Andersson B. D. Velie L. S. Andersson G. Lindgren 《Animal genetics》2017,48(6):694-697
The Icelandic horse is a breed known mainly for its ability to perform the ambling four‐beat gait ‘tölt’ and the lateral two‐beat gait pace. The natural ability of the breed to perform these alternative gaits is highly desired by breeders. Therefore, the discovery that a nonsense mutation (C>A) in the DMRT3 gene was the main genetic factor for horses' ability to perform gaits in addition to walk, trot and canter was of great interest. Although several studies have demonstrated that homozygosity for the DMRT3 mutation is important for the ability to pace, only about 70% of the homozygous mutant (AA) Icelandic horses are reported to pace. The aim of the study was to genetically compare four‐ and five‐gaited (i.e. horses with and without the ability to pace) AA Icelandic horses by performing a genome‐wide association (GWA) analysis. All horses (n = 55) were genotyped on the 670K Axiom Equine Genotyping Array, and a GWA analysis was performed using the genabel package in r . No SNP demonstrated genome‐wide significance, implying that the ability to pace goes beyond the presence of a single gene variant. Despite its limitations, the current study provides additional information regarding the genetic complexity of pacing ability in horses. However, to fully understand the genetic differences between four‐ and five‐gaited AA horses, additional studies with larger sample materials and consistent phenotyping are needed. 相似文献