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31.
Several apiaceous and two asteraceous species were tested for their suitability to support larval development of the carrot fly. Plants grown in pots or transplanted from seed beds into pots, were inoculated with a specific number of eggs. Pupae and non-pupated larvae were collected 6–7 wk after inoculation. Both the number and weights of pupae produced varied widely among the species. Cultivated carrots Daucus carota sativus often gave rise to only moderate numbers of pupae, but these invariably attained the highest weights. Pimpinella major was the only apiaceous plant tested that did not yield any carrot flies. The two asteraceous plants Cichorium intybus and Tanacetum vulgare failed to support larval development. Total carrot fly biomass produced per plant was influenced by both the host species and the root weight. Emergence rates of adult flies were positively correlated with pupal weights. Small individuals tended to have a longer total developmental time from egg to adult fly. 相似文献
32.
Tetrodotoxin Interference of CNS Excitation by Glutamic Acid 总被引:2,自引:0,他引:2
TETRODOTOXIN (TTX), the powerful neurotoxin of the puffer fish, blocks electrical excitation of many neuronal and muscle membranes1. This neurotoxin selectively blocks the sodium conductance increase, leaving the delayed potassium conductance change unaltered2–6. 相似文献
33.
Respiration in Saccharomyces cerevisiae is regulated by small proteins such as the respiratory supercomplex factors (Rcf). One of these factors (Rcf1) has been shown to interact with complexes III (cyt. bc1) and IV (cytochrome c oxidase, CytcO) of the respiratory chain and to modulate the activity of the latter. Here, we investigated the effect of deleting Rcf1 on the functionality of CytcO, purified using a protein C-tag on core subunit 1 (Cox1). Specifically, we measured the kinetics of ligand binding to the CytcO catalytic site, the O2-reduction activity and changes in light absorption spectra. We found that upon removal of Rcf1 a fraction of the CytcO is incorrectly assembled with structural changes at the catalytic site. The data indicate that Rcf1 modulates the assembly and activity of CytcO by shifting the equilibrium of structural sub-states toward the fully active, intact form. 相似文献
34.
E. Iwarsson Monalill Lundqvist José Inzunza Lars Ährlund-Richter Peter Sjöblom Örjan Lundkvist Niklas Simberg Magnus Nordenskjöld Elisabeth Blennow 《Human genetics》1999,104(5):376-382
We have studied the chromosomal content in 68 normally fertilised freeze-thawed human embryos of good morphology from 34
patients with an average maternal age of 32,6 years. Forty embryos showed post-thaw cellular division and twenty-eight post-thaw
cleavage arrest. After spreading of the embryos on microscope slides, analysis of chromosomes X, Y, 15, 16, 17 and 18 was
performed using two rounds of fluorescent in situ hybridisation (FISH). According to the results, the embryos were divided into four groups: (I) normal, all nuclei uniformly
diploid, (II) diploid mosaics, normal diploid blastomeres in combination with abnormal blastomeres, (III) abnormal, all nuclei
abnormal, (IV) chaotic, the chromosome constitution varies randomly from cell to cell. Approximately 25% of the embryos had
normal number of the chromosomes tested, while the majority of the embryos were abnormal. Most of the abnormal embryos were
diploid mosaics (57%). This was true for the embryos showing cleavage division as well as the embryos showing cleavage arrest.
Our data show a slightly higher incidence of abnormal embryos compared to those obtained with FISH in non-cryopreserved embryos
and confirm that the majority of preimplantation embryos fertilised in vitro contain abnormal blastomeres. The results, mechanisms, significance and implications are discussed.
Received: 19 November 1998 / Accepted: 4 March 1999 相似文献
35.
K. TYNKKYNEN K. J. RAATIKAINEN M. HÄKKILÄ E. HAUKILEHTO J. S. KOTIAHO 《Journal of evolutionary biology》2009,22(12):2512-2518
One explanation for hybridization between species is the fitness benefits it occasionally confers to the hybridizing individuals. This explanation is possible in species that have evolved alternative male reproductive tactics: individuals with inferior tactics might be more prone to hybridization provided it increases their reproductive success and fitness. Here we experimentally tested whether the propensity of hybridization in the wild depends on male reproductive tactic in Calopteryx splendens damselflies. Counter to our expectation, it was males adopting the superior reproductive tactic (territoriality) that had greatest propensity to hybridize than males adopting the inferior tactics (sneakers and floaters). Moreover, among the territorial males, the most ornamented males had greatest propensity to hybridize whereas the pattern was reversed in the sneaker males. Our results suggest that there is fluctuating selection on male mate discrimination against heterospecific females depending on both ornament size and the male’s reproductive tactic. 相似文献
36.
37.
RALF B. SCHÄFER STEVEN J. COOKE ROBERT ARLINGHAUS NÚRIA BONADA FRANÇOIS BRISCHOUX ANDREW F. CASPER JANE A. CATFORD VIRGINIE ROLLAND 《Freshwater Biology》2011,56(11):2405-2412
1. Two senior ecologists summarised their experience of the scientific publication process ( Statzner & Resh, Freshwater Biology, 2010 ; 55 , 2639) to generate discussion, particularly among early career researchers (ECRs). As a group of eight ECRs, we comment on the six trends they described. 2. We generally agree with most of the trends identified by Statzner & Resh (2010) , but also highlight a number of divergent perspectives and provide recommendations for change. Trends of particular concern are the use of inappropriate metrics to evaluate research quality (e.g. impact factor) and the salami slicing of papers to increase paper count. We advocate a transparent and comprehensive system for evaluating the research. 3. We stress the importance of impartiality and independence in the peer review process. We therefore suggest implementation of double‐blind review and quality control measures for reviewers and possibly editors. Besides such structural changes, editors should be confident to overrule biased reviewer recommendations, while reviewers should provide helpful reviews but be explicit if a submission does not meet quality standards. Authors should always conduct a thorough literature search and acknowledge historical scientific ideas and methods. Additionally, authors should report low‐quality copy editing and reviews to the editors. 4. Both early and late career researchers should jointly implement these recommendations to reverse the negative trends identified by Statzner & Resh (2010) . However, more senior scientists will always have to take the lead with respect to structural changes in the publication system given that they occupy the majority of decision‐making positions. 相似文献
38.
39.
Water relations and carbon gain are closely related to cushion size in the moss Grimmia pulvinata 总被引:1,自引:0,他引:1
The present study of structural and physiological changes during the development of the cushion moss, Grimmia pulvinata , quantifies the size-dependence of various parameters of water relations such as changes in surface: volume ratio ( S/V ) or water loss rates, and also measures net CO2 gas exchange in the light and the dark. Larger cushions had lower S/V values than smaller ones and featured lower rates of area-based evapotranspiration, owing to higher boundary-layer resistance, but did not differ in relative water storage capacity (expressed as a percentage of d. wt). In combination, this leads to considerably longer hydration periods in larger cushions. By contrast, CO2 gas-exchange parameters were negatively correlated with size : larger cushions showed significantly lower (mass-based) rates of net photosynthesis and dark respiration. Using these data, we estimated carbon budgets during a drying cycle as a function of cushion size. When including alternations of dark and light periods, the relationship proved to be rather complicated. Depending on the time of hydration, net carbon budgets not only varied quantitatively with size but sometimes took on both positive and negative values depending on cushion size. We conclude that neglecting plant size can lead to unrepeatable or even misleading results in comparative ecophysiological studies, and therefore urge for adequate attention to be paid to size in these studies. 相似文献
40.
PETRI P. KÄRENLAMPI 《Biological journal of the Linnean Society. Linnean Society of London》2012,106(3):689-697
Species extinction in finite random replicator systems is investigated as a function of within‐species interaction pressure. Extinctions are shown to increase with increased symmetry of among‐species interactions. The proportion of extinct species increases with increased system size. The effect of system connectance is reduced to a variability effect: connectivity geometry appears unimportant as long as closed subspaces are not formed. A small system can be interpreted as a closed subsystem of a larger system, which allows size‐scaling of the interaction pressure. At large interaction pressure, the scaling unifies the proportion of extinct species. At the limit of small within‐species interaction pressure, a small number of species survive, and the proportion of extinct species shows a system size effect. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 106 , 689–697. 相似文献