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21.
C. T. SMITH J. BAKER L. PARK L. W. SEEB C. ELFSTROM S. ABE J. E. SEEB 《Molecular ecology resources》2005,5(2):259-262
We report the characterization of 13 single nucleotide polymorphism (SNP) genotyping assays for chum salmon (Oncorhynchus keta). These assays are based on the 5′‐nuclease reaction and thus facilitate high‐throughput genotyping with minimal optimization time. Because data generated using these markers may be transported and combined across laboratories, SNPs offer the potential to reduce the amount of redundant work being done in mixture and migratory studies of chum salmon. 相似文献
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The development time, sex ratio and offspring production of Melittobia clavicornis reared with wild, facultative and factitious hosts are presented. Known hosts and different biological aspects presented in previously published reports are summarized and clarified. Immature development time was equivalent or slightly longer than that of other Melittobia species, and the sex ratio was approximately 97% female. Total offspring numbers were considerably lower than that of other Melittobia species using the same hosts. We report female dimorphism in this species for the first time. The number and relative proportion of brachypterous morph females produced was higher than that in other species of the genus. 相似文献
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ABE Y 《Acta pathologica et microbiologica Scandinavica》1948,25(5):31-33
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YOSHIHIKO ABE 《The Journal of eukaryotic microbiology》1980,27(4):372-374
SYNOPSIS. Promastigotes of Leptomonas sp., a flagellate parasite of the silkworm, Bombyx mori , multiplied by binary fission with the following sequence of events: duplication of the flagellum; division of the kinetoplast and the nucleus; spatial separation of the kinetoplast: and cytokinesis resulting in the formation of 2 daughter promastigotes. In the early stages of encystment, promastigotes aggregated in a rosette and assumed a stumpy form. The nucleus and kinetoplast of the stumpy promastigotes were double, suggesting a possibility of fusion of the organism in the rosette. When most of the promastigotes in the cluster became stumpy, each individual was isolated from the cluster and acquired a thick coat with an acidophilic substance, thus forming a cyst. 相似文献
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Polyphenylalamine synthesis by cytoplasmic ribosomes of Gateway barley (Hordeum vulgare) and its virescens single gene nuclear mutant was compared. The cytoplasmic 80S ribosomes were isolated from unimbibed embryo material and the ribosomes were dissociated into their component 60S and 40S subunits by centrifugation through sucrose gradients containing high KCl-to-MgCl2 buffer. These separated subunits could be reassociated by resuspension in buffer having about equimolar concentrations of MgCl2 and KCl. Both homologous and heterologous combinations of the subunits reassociated to give monomeric 80S ribosomes, and the derived monomers as well as various combinations of the individual subunits showed equivalent activity in an in vitro system for poly (U)-directed polyphenylalanine synthesis. 相似文献
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Various agents were tested for the effects on both the electrofusionand the subsequent rounding up (spherulation) of fused protoplastsfrom barley (Hordeum vulgare var. Moor). The microfilament (MF)inhibitor, cytochalasin D (CD), had no effect on the frequencyof fusion, but greatly increased the frequency of spherulation.The effects of CD were rapid, long-lived and maximal at 2 to10 mmol m3. Ca2+ also promoted spherulation of fusionproducts, whereas phalloidin and the calcium inonophore A23187completely abolished the effect of both CD and Ca2+. An internalCa2+ antagonist, 8-(diethylamino)octyl 3, 4, 5-trimethoxybenzoate(TMB8) at 50 mmol m3 also inhibited spherulation withoutaffecting the frequency of fusion and CD could almost completelyreverse its effects. The effects of CD persisted for up to 1h after its removal, whereas the effects of Ca2+ and TMB8 wereexerted at the beginning of incubation and were immediatelyabolished by washing. These results indicate that the effectof Ca2+ on the formation of spherical fusion products is closelycorrelated with the status of the microfilaments and that theinternal Ca2+ concentration and, perhaps, its transient changewhich affects the MF, are intimately involved in the process. Key words: Cytoskeleton, electrofusion, calcium effect 相似文献