MIDDLE ORDOVICIAN MOLLUSCS FROM THE PAIYANGHO REGION,CHILIENSHAN(“NANSHAN”)

INTRODUCTIONThis paper deals with all the fossil molluscs collected respectively by Dr.P.Li's party in1956 and Dr.T.H.Yin's party in 1957 of the Institute of Geology,Academia Sinica.Pre-liminary reports regarding the discovery of the Ordovician in the Paiyangho region has been madeby Li,and Yang(1957),and Li,Hsieh and Ho(1958),while the writer had twice reported     

大豆灰斑病抗性遗传的三点测交分析

本实验利用三点测交分析的方法, 对3个组合在人工接种大豆灰斑病菌的条件下的抗性表现进行基因效应分析,各组合均存在加性,组合1存在显性,组合2、3存在上位性。 Abstract:In this paper,Triple Test Cross Design was used in studing the resistance of soybean to 10 physiological race of Cercospora Sojina Haraby inoculation.Results o analysis of gene effects of resistance indicated that additive effect is significant in all the three crosses,dominant effect exsists only in the cross 1 and epistatic effect remains in the cross 2 and cross 3.     

RFLP诊断方法的改进

RFLP(限制性片段长度多态)技术应用于遗传病的诊断始于1981年。在十年时间里,这一基因诊断技术和分析方法不断得到发展和改进。随着越来越多的致病基因和连锁DNA多态片段的分离和克隆,该技术在分析缺陷基因性质,携带者检出和产前诊断等方面将发挥重要作用.然而,RFLP技术复杂,耗费时间和费用较大,使其向临床推广应用面临很大局限性。因此,基因诊断的方法简化和技术改进大有必要。1985年首创的多聚酶链     

不同形式混合资料遗传力的估计方法

本文探讨了用一种特殊形式资料估计遗传力的方法,亦可作为为扩大样本含量,充分利用不同来源且形式不同资料合并估计遗传力的方法。     

连锁值——表述基因连锁强度的新参数

在遗传学中,同一染色体上基因之间的连锁强度是用交换率表示的.一般而言,交换率越高,基因连锁强度越低.但交换率不是基因连锁强度的最方便的量度,因为利用交换率来计算具有连锁交换现象的杂交后代分离比例时,必须区分相引组和相斥组两种不同的亲本交配情形.在相引组,一个亲本具有两个显性性状,另一个亲本具有两个隐性性状;在相斥组,两个亲本各具有一个显性性状和一个隐性性状。这两种情形下的配子比例和F_2分     

Call Characteristic Network Reveal Geographical Patterns of Call Similarity:Applying Network Analysis to Frog’s Call Research

Individual’s phenotypic traits are the results of adaptation to ecological conditions.Therefore,different selection pressures caused by heterogeneous environments may result in phenotypic difference,especially for individuals in different geographical populations.Here,we illustrated for the first time to use social network analysis(SNA)for examining whether geographical proximity predicts the similarity patterns in call characteristics among populations of an anuran species.We recorded calls from 150 male dorsal-striped opposite-fingered treefrogs(Chiromantis doriae)at 11 populations in Hainan Province and one population in Guangdong Province in China's Mainland,and we measured eight acoustic variables for each male.Mantel test didn’t show a correlation between geographical proximity and the similarity in call characteristics among populations.In addition,we failed to find correlations between a population’s eigenvector centrality and the distance to its nearest neighbor,nor between the coefficient of variation of similarity in call characteristics of a population and the average distance to all other populations.Nevertheless,three acoustic clusters were identified by the Girvan-Newman algorithm,and clustering was partially associated with geography.Furthermore,the most central populations were included in the same cluster,but the top betweenness populations were located within different clusters,suggesting that centrality populations are not necessary bridging between clusters.These results demonstrate the potential usefulness of the SNA toolbox and indicate that SNA helps to uncover the patterns that often overlooked in other analytical methods.By using SNA in frog’s call studies,researchers could further uncover the potential relationship in call characteristics between geographical populations,further reveal the effects of ecological factors on call characteristics,and probably enhance our understanding of the adaptive evolution of acoustic signals.     

Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma

For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP.     

A novel strategy to inhibit the reproduction and translation of hepatitis C virus

Hepatitis C virus (HCV), a positive single-stranded RNA virus, is a major cause of liver disease in humans. Herein we report a novel strategy to inhibit the reproduction and translation of HCV using a short RNA, named an Additional RNA, to activate the endonuclease activity of Argonaute 2 (Ago2). In the presence of the Additional RNA, the HCV genome RNA has the requisite 12 nucleotides of base-pairing with microRNA-122. This activates the endonuclease activity of Ago2, resulting in cleavage and release of the HCV genome RNA from Ago2 and microRNA-122. The free HCV genome RNA would be susceptible to intracellular degradation, effectively inhibiting its reproduction and translation. This study presents a new method to inhibit HCV that may hold great potential for HCV treatment in the future.     

广布中剑水蚤对裸腹和秀体的摄食(英文)

１９９５年６月至９月,在武汉东湖,通过摄食实验和野外调查,作者研究了广布中剑水蚤对裸腹和秀体的摄食。研究结果证实,广布中剑水蚤能够抓取和摄食裸腹和秀体。广布中剑水蚤对小型裸腹的平均摄食率高于大型裸腹。多重统计分析结果表明,广布中剑水蚤对小型和大型裸腹的摄食率有显著差异（Ｐ＝００３３５）,捕食者喜食小型猎物。由于广布中剑水蚤的摄食习性,它对裸腹和秀体的种群动态有重要影响,但随着鱼产量增加,广布中剑水蚤对猎物摄食的影响效果被减弱了。