Advances in the reintroduction of rare and endangered wild plant species

Human disturbance and climate change have increased the risk of extinction for rare and endangered wild plant species.One effective way to conserve these rare and endangered species is through reintroduction.In this review,we summarize the advances in wild plant reintroduction from five perspectives:the establishment of reintroduction biology as an important tool for biodiversity conservation;the importance of genetic diversity in reintroduction;reintroduction under global climate change;recruitment limitation in reintroduction;and reintroduction and ecological restoration.In addition,we consider the future of plant reintroduction strategies.     

Erratum to: Maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip,faba bean,chickpea, and soybean

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Effects of Temperature and pH on Cell Growth of a Flocculating Microalga (Chlorella vulgaris) JSC-7

微藻是可广泛用于健康食品及水产养殖的饵料,同时,微藻细胞内积累的油脂可作为可再生生物燃料,因此微藻的生长和代谢受到广泛关注。温度和pH对微藻的生物量积累有很大影响,考察不同温度和pH条件下微藻细胞的生长有助于寻找最佳的条件进行微藻的培养。自絮凝小球藻JSC-7（Chlorella vulgaris JSC-7）可实现自沉降采收,有利于降低微藻生产成本,优化其生长条件对更好地利用该微藻具有重要意义。考察了温度（22∽40℃）及pH（6.0∽10.0）对其细胞生长、叶绿素含量和油脂产量的影响。在所选取的温度及pH范围内,JSC-7细胞均可生长,显示该藻种可以适应广泛的温度和pH条件。适合细胞生长的温度依次为31℃〉28℃〉35℃〉25℃,pH依次为7.0〉8.0〉6.0。pH 8.0时生物量和油脂的积累量最多,说明该藻株在弱碱条件下更适合生长和产油。当温度为31℃、pH为7.0时,可获得最高的生长量（OD690=0.941）、叶绿素含量（19 mg/L）及油脂产量（39.07%/克干重）。     

一步法提取植物DNA用于大规模RAPD分析

ＲＡＰＤ技术已广泛地用于植物的系统演化、种群多样性、群体遗传学及杂种种子纯度的鉴定等工作中〔１～３〕。本文基于快速节省的原则对碱液法提取植物ＤＮＡ进行一些改进。１材料与方法ＤＮＡ提取方法取１０～２０ｍｇ幼叶 ,加１００μｌ０ ５ｍｏｌ／ＬＮａＯＨ（或附加０ ５ %～１ ５ %巯基乙醇）研磨后 ,取４０μｌ研磨液加入２００μｌ１００ｍｍｏｌ／ＬＴｒｉｓ ＨＣｌｐＨ７ ６缓冲液（或附加０ ５%不溶性聚乙烯吡咯烷酮 ,ＰＶＰ）中 ,８０００×ｇ离心５ｍｉｎ,上清液即可用于ＰＣＲ反应。ＰＣＲ反应体系含１０ｍｍｏｌ／ＬＴｒｉ…     

Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells

INTRODUCTIONTelomerase is a ribonucleoprotein complex that plays a critical role in telomeremaintenance and cellular immortality. Telomerase has been considered as tumordiagnostic marker and potential target for cancer therapyll, 2]. A sensitive, reliableand quantitative assay is of high interest in this field. The development of a very sensitive Telomeric Repeat Amplification Protocol (TRAP) for measuring telomerajseactivity in cell extracts has been proved to be an important tool for…     

Establishment of HIV-1 model cell line GHOST(3) with stable DRiP78 and NHERF1 knockdown

Chemokine receptors CXCR4 and CCR5 are indispensable co-receptors for HIV-1 entry into host cells. In our previous study, we identified that dopamine receptor-interacting protein 78(DRi P78) and Na+-H+ exchanger regulatory factor 1(NHERF1) are the CXCR4 and CCR5 homo- or hetero-dimerinteracting proteins. DRi P78 and NHERF1 are able to influence the co-receptor internalization and intracellular trafficking. Over-expression of NHERF1 affects the ligands or HIV-1 gp120-induced CCR5 internalization and HIV-1 production. It is reasonable to speculate that DRi P78 and NHERF1, as well as the signaling pathways involved in viral replication, would probably affect HIV-1 replication through regulating the co-receptors. In this present study, we designed two short hairpin RNAs(sh RNAs) targeting the DRi P78 and NHERF1, respectively, and constructed the p Lenti6/BLOCK-i T-DEST lentiviral plasmids expressing DRi P78 or NHERF1 sh RNA. The packaged lentiviruses were used to transduce the widely-applied HIV-1 model cell line GHOST(3). Then, cells with stable knockdown were established through selecting transduced cells with Blasticidin. This study, for the first time, reported the establishment of the GHOST(3) with DRi P78 and NHERF1 knockdown, which is the first stable cell line with HIV-1 co-receptor-interacting molecular defects.     

印度谷螟突变体的诱导与培育

1991─1993年, 借助60 Co辐照及杂交技术,在国内外首次诱导和培育出印度谷螟(Plodia interpunctella Hubner)成虫的15种突变体。这些突变体是:翅透明型,半翅透明型,翅端部透明型,翅半月状透明斑型,前翅内半透明型, 后翅透明型,非对称透明型,深红眼型,淡红眼型,白眼型,胸腹板、足黄色型,深红眼翅透明型,淡红眼翅透明型,白眼翅透明型及眼翅腹三特征突变体。 Abstract:15 mutants of adults of Indian meal Moth,Plodia interpunctella Hubner,were obtained through 60Co γ-ray irradiation and hybridizations.These mutants are:transparent wings(tw),transparent semi-wings(tsw),transparent a thord outer region of wings(ttorw),semilunar transparent wings(stw),transparent base half of front wings(tbhfw),transparent hind wings(thw),non-parallel transparent wings(nptw),deep red eyes(dre),light red eyes(lre),white eyes(we),yellow feets and troracic sternum(yfts),deep red eyes and transparent wings(dre-tw),light red eyes and transparent wings(lre-tw),white eyes and transparent wings(we-tw),red eyes-transparent wings-transparent 5-6 th abdominal segments(re-tw-tas).